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Article: Tumor necrosis factor mRNA localized to Paneth cells of normal murine intestinal epithelium by in situ hybridization

TitleTumor necrosis factor mRNA localized to Paneth cells of normal murine intestinal epithelium by in situ hybridization
Authors
Issue Date1990
PublisherRockefeller University Press. The Journal's web site is located at http://www.jem.org
Citation
Journal Of Experimental Medicine, 1990, v. 171 n. 1, p. 327-332 How to Cite?
AbstractPaneth cells in normal murine small intestine contain TNF mRNA that is readily detectable by in situ hybridization, unlike resident macrophages in lamina propria, which are negative. Northern blot analysis of whole tissue shows the presence of mRNA that has the same electrophoretic mobility as TNF mRNA from activated macrophages. A low level of TNF bioactivity, but no immunoreactivity, was detected in normal small intestine, and TNF production in resting Paneth cells appears to be post-transcriptionally controlled. Typical leukocyte surface membrane markers were not found on Paneth cells, but were expressed by the surrounding lamina propria macrophages. Paneth cells are thus epithelial cells with leukocyte-like secretory potential that may be important in intestinal physiology and pathology.
Persistent Identifierhttp://hdl.handle.net/10722/176000
ISSN
2015 Impact Factor: 11.24
2015 SCImago Journal Rankings: 10.762
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorKeshav, Sen_US
dc.contributor.authorLawson, Len_US
dc.contributor.authorChung, LPen_US
dc.contributor.authorStein, Men_US
dc.contributor.authorPerry, VHen_US
dc.contributor.authorGordon, Sen_US
dc.date.accessioned2012-11-26T09:04:03Z-
dc.date.available2012-11-26T09:04:03Z-
dc.date.issued1990en_US
dc.identifier.citationJournal Of Experimental Medicine, 1990, v. 171 n. 1, p. 327-332en_US
dc.identifier.issn0022-1007en_US
dc.identifier.urihttp://hdl.handle.net/10722/176000-
dc.description.abstractPaneth cells in normal murine small intestine contain TNF mRNA that is readily detectable by in situ hybridization, unlike resident macrophages in lamina propria, which are negative. Northern blot analysis of whole tissue shows the presence of mRNA that has the same electrophoretic mobility as TNF mRNA from activated macrophages. A low level of TNF bioactivity, but no immunoreactivity, was detected in normal small intestine, and TNF production in resting Paneth cells appears to be post-transcriptionally controlled. Typical leukocyte surface membrane markers were not found on Paneth cells, but were expressed by the surrounding lamina propria macrophages. Paneth cells are thus epithelial cells with leukocyte-like secretory potential that may be important in intestinal physiology and pathology.en_US
dc.languageengen_US
dc.publisherRockefeller University Press. The Journal's web site is located at http://www.jem.orgen_US
dc.relation.ispartofJournal of Experimental Medicineen_US
dc.subject.meshAnimalsen_US
dc.subject.meshDna - Geneticsen_US
dc.subject.meshEpithelial Cellsen_US
dc.subject.meshEpithelium - Metabolismen_US
dc.subject.meshFemaleen_US
dc.subject.meshImmunoenzyme Techniquesen_US
dc.subject.meshIntestinal Mucosa - Cytology - Metabolismen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred C57blen_US
dc.subject.meshNucleic Acid Hybridizationen_US
dc.subject.meshRna, Messenger - Analysis - Geneticsen_US
dc.subject.meshTumor Necrosis Factor-Alpha - Geneticsen_US
dc.titleTumor necrosis factor mRNA localized to Paneth cells of normal murine intestinal epithelium by in situ hybridizationen_US
dc.typeArticleen_US
dc.identifier.emailChung, LP: lpchung@hkucc.hku.hken_US
dc.identifier.authorityChung, LP=rp00249en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1084/jem.171.1.327en_US
dc.identifier.pmid2404082-
dc.identifier.scopuseid_2-s2.0-0025133975en_US
dc.identifier.volume171en_US
dc.identifier.issue1en_US
dc.identifier.spage327en_US
dc.identifier.epage332en_US
dc.identifier.isiWOS:A1990CH51000025-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridKeshav, S=7003367734en_US
dc.identifier.scopusauthoridLawson, L=7103094744en_US
dc.identifier.scopusauthoridChung, LP=24315879100en_US
dc.identifier.scopusauthoridStein, M=7402996760en_US
dc.identifier.scopusauthoridPerry, VH=7101787946en_US
dc.identifier.scopusauthoridGordon, S=35391350600en_US

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