File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: A role for mitogen-activated protein kinase and Ets-1 in the induction of interleukin-10 transcription by human immunodeficiency virus-1 Tat

TitleA role for mitogen-activated protein kinase and Ets-1 in the induction of interleukin-10 transcription by human immunodeficiency virus-1 Tat
Authors
Issue Date2007
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/IMM
Citation
Immunology, 2007, v. 121 n. 3, p. 337-348 How to Cite?
AbstractThe human immunodeficiency virus (HIV) Tat protein has multiple regulatory roles, including trans-activation of the HIV genome and regulation of immune signalling processes, including kinase activation and cytokine expression. We recently demonstrated that HIV-1 Tat induces the expression of interleukin (IL)-10 via p38 mitogen-activated protein kinase (MAPK) activation. We further delineated that the Tat-responsive element of the IL-10 promoter was located within 625 to 595 bp upstream from the transcription start site. Using electrophoretic mobility shift assays, the transcription factors Ets-1 and Sp-1 were shown to bind to the IL-10 promoter to activate transcription of the gene. Furthermore, sequential deletional mutations of the Ets-1- and Sp-1-binding sites in the -625/-595 region reduced the DNA binding and transcription activity of the IL-10 promoter. Our results also showed that both the Tat-induced and Ets-1-regulated IL-10 promoter-driven luciferase activity can be abrogated by inhibitors of the p38 MAPK activity. In conclusion, the coordinated activities of p38 MAPK and the transcription factors, Ets-1 and Sp-1, may play an important role in the HIV-1 Tat-induced IL-10 transcription. © 2007 Blackwell Publishing Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/175964
ISSN
2015 Impact Factor: 4.078
2015 SCImago Journal Rankings: 2.038
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, JCBen_US
dc.contributor.authorLau, ASYen_US
dc.date.accessioned2012-11-26T09:03:03Z-
dc.date.available2012-11-26T09:03:03Z-
dc.date.issued2007en_US
dc.identifier.citationImmunology, 2007, v. 121 n. 3, p. 337-348en_US
dc.identifier.issn0019-2805en_US
dc.identifier.urihttp://hdl.handle.net/10722/175964-
dc.description.abstractThe human immunodeficiency virus (HIV) Tat protein has multiple regulatory roles, including trans-activation of the HIV genome and regulation of immune signalling processes, including kinase activation and cytokine expression. We recently demonstrated that HIV-1 Tat induces the expression of interleukin (IL)-10 via p38 mitogen-activated protein kinase (MAPK) activation. We further delineated that the Tat-responsive element of the IL-10 promoter was located within 625 to 595 bp upstream from the transcription start site. Using electrophoretic mobility shift assays, the transcription factors Ets-1 and Sp-1 were shown to bind to the IL-10 promoter to activate transcription of the gene. Furthermore, sequential deletional mutations of the Ets-1- and Sp-1-binding sites in the -625/-595 region reduced the DNA binding and transcription activity of the IL-10 promoter. Our results also showed that both the Tat-induced and Ets-1-regulated IL-10 promoter-driven luciferase activity can be abrogated by inhibitors of the p38 MAPK activity. In conclusion, the coordinated activities of p38 MAPK and the transcription factors, Ets-1 and Sp-1, may play an important role in the HIV-1 Tat-induced IL-10 transcription. © 2007 Blackwell Publishing Ltd.en_US
dc.languageengen_US
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/IMMen_US
dc.relation.ispartofImmunologyen_US
dc.rightsImmunology. Copyright © Blackwell Publishing Ltd.-
dc.subject.meshBase Sequenceen_US
dc.subject.meshBinding Sitesen_US
dc.subject.meshCell Lineen_US
dc.subject.meshDna-Binding Proteins - Metabolismen_US
dc.subject.meshGene Products, Tat - Immunologyen_US
dc.subject.meshHiv-1 - Immunologyen_US
dc.subject.meshHela Cellsen_US
dc.subject.meshHumansen_US
dc.subject.meshInterleukin-10 - Geneticsen_US
dc.subject.meshPromoter Regions, Genetic - Immunologyen_US
dc.subject.meshProto-Oncogene Protein C-Ets-1 - Genetics - Immunologyen_US
dc.subject.meshRecombinant Proteins - Immunologyen_US
dc.subject.meshSignal Transduction - Genetics - Immunologyen_US
dc.subject.meshTranscription, Genetic - Immunologyen_US
dc.subject.meshP38 Mitogen-Activated Protein Kinases - Immunologyen_US
dc.subject.meshTat Gene Products, Human Immunodeficiency Virusen_US
dc.titleA role for mitogen-activated protein kinase and Ets-1 in the induction of interleukin-10 transcription by human immunodeficiency virus-1 Taten_US
dc.typeArticleen_US
dc.identifier.emailLi, JCB: jamesli@hku.hken_US
dc.identifier.emailLau, ASY: asylau@hku.hken_US
dc.identifier.authorityLi, JCB=rp00496en_US
dc.identifier.authorityLau, ASY=rp00474en_US
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1111/j.1365-2567.2007.02580.xen_US
dc.identifier.pmid17376198-
dc.identifier.pmcidPMC2265950-
dc.identifier.scopuseid_2-s2.0-34249327710en_US
dc.identifier.hkuros126928-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34249327710&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume121en_US
dc.identifier.issue3en_US
dc.identifier.spage337en_US
dc.identifier.epage348en_US
dc.identifier.eissn1365-2567-
dc.identifier.isiWOS:000246793800005-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridLi, JCB=23103447500en_US
dc.identifier.scopusauthoridLau, ASY=7202626202en_US
dc.identifier.citeulike1341416-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats