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Article: Evidence that RNA editing modulates splice site selection in the 5-HT2C receptor gene

TitleEvidence that RNA editing modulates splice site selection in the 5-HT2C receptor gene
Authors
Issue Date2004
PublisherOxford University Press. The Journal's web site is located at http://nar.oxfordjournals.org/
Citation
Nucleic Acids Research, 2004, v. 32 n. 7, p. 2113-2122 How to Cite?
AbstractAdenosine to inosine editing of mRNA from the human 5-HT2C receptor gene (HTR2C) occurs at five exonic positions (A-E) in a stable stem-loop that includes the normal 5′ splice site of intron 5 and is flanked by two alternative splice sites. Using in vitro editing, we identified a novel editing site (F) located in the intronic part of the stem-loop and demonstrated editing at this site in human brain. We have shown that in cell culture, base substitutions to mimic editing at different combinations of the six sites profoundly affect relative splicing at the normal and the upstream alternative splice site, but splicing at the downstream alternative splice site was consistently rare. Editing combinations in different splice variants from human brain were determined and are consistent with the effects of editing on splicing observed in cell culture. As RNA editing usually occurs close to exon/intron boundaries, this is likely to be a general phenomenon and suggests an important novel role for RNA editing. © Oxford University Press 2004; all rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/175937
ISSN
2015 Impact Factor: 9.202
2015 SCImago Journal Rankings: 7.458
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorFlomen, Ren_US
dc.contributor.authorKnight, Jen_US
dc.contributor.authorSham, Pen_US
dc.contributor.authorKerwin, Ren_US
dc.contributor.authorMakoff, Aen_US
dc.date.accessioned2012-11-26T09:02:39Z-
dc.date.available2012-11-26T09:02:39Z-
dc.date.issued2004en_US
dc.identifier.citationNucleic Acids Research, 2004, v. 32 n. 7, p. 2113-2122en_US
dc.identifier.issn0305-1048en_US
dc.identifier.urihttp://hdl.handle.net/10722/175937-
dc.description.abstractAdenosine to inosine editing of mRNA from the human 5-HT2C receptor gene (HTR2C) occurs at five exonic positions (A-E) in a stable stem-loop that includes the normal 5′ splice site of intron 5 and is flanked by two alternative splice sites. Using in vitro editing, we identified a novel editing site (F) located in the intronic part of the stem-loop and demonstrated editing at this site in human brain. We have shown that in cell culture, base substitutions to mimic editing at different combinations of the six sites profoundly affect relative splicing at the normal and the upstream alternative splice site, but splicing at the downstream alternative splice site was consistently rare. Editing combinations in different splice variants from human brain were determined and are consistent with the effects of editing on splicing observed in cell culture. As RNA editing usually occurs close to exon/intron boundaries, this is likely to be a general phenomenon and suggests an important novel role for RNA editing. © Oxford University Press 2004; all rights reserved.en_US
dc.languageengen_US
dc.publisherOxford University Press. The Journal's web site is located at http://nar.oxfordjournals.org/en_US
dc.relation.ispartofNucleic Acids Researchen_US
dc.subject.meshAgeden_US
dc.subject.meshAged, 80 And Overen_US
dc.subject.meshAlternative Splicing - Geneticsen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshBinding Sites - Geneticsen_US
dc.subject.meshBrain - Metabolismen_US
dc.subject.meshCos Cellsen_US
dc.subject.meshCell Lineen_US
dc.subject.meshCell Line, Tumoren_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshIntrons - Geneticsen_US
dc.subject.meshMaleen_US
dc.subject.meshModels, Geneticen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshMutationen_US
dc.subject.meshPc12 Cellsen_US
dc.subject.meshRna - Genetics - Metabolismen_US
dc.subject.meshRna Editingen_US
dc.subject.meshRatsen_US
dc.subject.meshReceptor, Serotonin, 5-Ht2c - Geneticsen_US
dc.titleEvidence that RNA editing modulates splice site selection in the 5-HT2C receptor geneen_US
dc.typeArticleen_US
dc.identifier.emailSham, P: pcsham@hku.hken_US
dc.identifier.authoritySham, P=rp00459en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1093/nar/gkh536en_US
dc.identifier.pmid15087490-
dc.identifier.scopuseid_2-s2.0-2342460272en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-2342460272&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume32en_US
dc.identifier.issue7en_US
dc.identifier.spage2113en_US
dc.identifier.epage2122en_US
dc.identifier.isiWOS:000221145400007-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridFlomen, R=6603223003en_US
dc.identifier.scopusauthoridKnight, J=13002769800en_US
dc.identifier.scopusauthoridSham, P=34573429300en_US
dc.identifier.scopusauthoridKerwin, R=7102904567en_US
dc.identifier.scopusauthoridMakoff, A=7006063526en_US

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