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Article: Linkage disequilibrium mapping of chromosome 22 in schizophrenia using DNA pooling on Chinese and Scottish populations

TitleLinkage disequilibrium mapping of chromosome 22 in schizophrenia using DNA pooling on Chinese and Scottish populations
Authors
Issue Date1998
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0148-7299:1/
Citation
American Journal Of Medical Genetics - Neuropsychiatric Genetics, 1998, v. 81 n. 6, p. 471 How to Cite?
AbstractThere is good evidence from linkage analysis that there are several genetic loci for schizophrenia, including on chromosomes 6, 13q, 8p, and 22q. However, linkage alone has poor power to precisely localize the underlying susceptibility genes. Using chromosome 22 as a starting point, we have attempted to confirm and fine-map linked loci using linkage disequilibrium analysis of the entire chromosome with approximately 70 microsatellite markers (average spacing ∼500 Kb). Two clinical samples of schizophrenic patients were used, consisting of 180 family trios and 80 sibling pairs from Chengdu, SW China (n = 860), and 400 cases and 400 controls from the Highland and Grampian regions of Scotland (N = 800). To reduce the amount of genotyping to a practical level, DNA samples were pooled at equimolar concentration, using fluorescent quantification of the DNA. This resulted in three separate matched sets of pools from the Chinese parents and offspring, derived from 100 trios, 80 trios, and 80 sibling-pairs, and two sets of case-control pools from the Scottish sample. Fluorescently labelled markers were initially genotyped in one pooled sample set from each ethnic group (100 trios and 200 cases and controls) on the capillary polymer-based ABI 310 genetic analyzer. The integrated peak areas of allele traces from fluorograms were compared between parents and offspring or case-controls to detect potential differences in allele frequency or allele transmission. The majority of traces were very similar between the pairs of pools, and those markers which showed differences were genotyped in the additional pools, followed by individual genotyping of the samples for transmission disequilibrium or chi-square analysis to pinpoint areas of significant linkage disequilibrium.
Persistent Identifierhttp://hdl.handle.net/10722/175896
ISSN
2015 Impact Factor: 3.391
2015 SCImago Journal Rankings: 1.771

 

DC FieldValueLanguage
dc.contributor.authorCollier, DAen_US
dc.contributor.authorLi, Ten_US
dc.contributor.authorHu, Xen_US
dc.contributor.authorLiu, Xen_US
dc.contributor.authorMurray, RMen_US
dc.contributor.authorSham, PCen_US
dc.contributor.authorAsherson, Pen_US
dc.contributor.authorHill, Len_US
dc.contributor.authorNinomiya, Ten_US
dc.contributor.authorCraig, Ien_US
dc.contributor.authorOwen, Men_US
dc.contributor.authorPlomin, Ren_US
dc.contributor.authorBreen, Gen_US
dc.contributor.authorShaw, DJen_US
dc.contributor.authorSt Clair, Den_US
dc.date.accessioned2012-11-26T09:02:15Z-
dc.date.available2012-11-26T09:02:15Z-
dc.date.issued1998en_US
dc.identifier.citationAmerican Journal Of Medical Genetics - Neuropsychiatric Genetics, 1998, v. 81 n. 6, p. 471en_US
dc.identifier.issn1552-4841en_US
dc.identifier.urihttp://hdl.handle.net/10722/175896-
dc.description.abstractThere is good evidence from linkage analysis that there are several genetic loci for schizophrenia, including on chromosomes 6, 13q, 8p, and 22q. However, linkage alone has poor power to precisely localize the underlying susceptibility genes. Using chromosome 22 as a starting point, we have attempted to confirm and fine-map linked loci using linkage disequilibrium analysis of the entire chromosome with approximately 70 microsatellite markers (average spacing ∼500 Kb). Two clinical samples of schizophrenic patients were used, consisting of 180 family trios and 80 sibling pairs from Chengdu, SW China (n = 860), and 400 cases and 400 controls from the Highland and Grampian regions of Scotland (N = 800). To reduce the amount of genotyping to a practical level, DNA samples were pooled at equimolar concentration, using fluorescent quantification of the DNA. This resulted in three separate matched sets of pools from the Chinese parents and offspring, derived from 100 trios, 80 trios, and 80 sibling-pairs, and two sets of case-control pools from the Scottish sample. Fluorescently labelled markers were initially genotyped in one pooled sample set from each ethnic group (100 trios and 200 cases and controls) on the capillary polymer-based ABI 310 genetic analyzer. The integrated peak areas of allele traces from fluorograms were compared between parents and offspring or case-controls to detect potential differences in allele frequency or allele transmission. The majority of traces were very similar between the pairs of pools, and those markers which showed differences were genotyped in the additional pools, followed by individual genotyping of the samples for transmission disequilibrium or chi-square analysis to pinpoint areas of significant linkage disequilibrium.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0148-7299:1/en_US
dc.relation.ispartofAmerican Journal of Medical Genetics - Neuropsychiatric Geneticsen_US
dc.titleLinkage disequilibrium mapping of chromosome 22 in schizophrenia using DNA pooling on Chinese and Scottish populationsen_US
dc.typeArticleen_US
dc.identifier.emailSham, PC: pcsham@hku.hken_US
dc.identifier.authoritySham, PC=rp00459en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.scopuseid_2-s2.0-0345624574en_US
dc.identifier.volume81en_US
dc.identifier.issue6en_US
dc.identifier.spage471en_US
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridCollier, DA=26642980600en_US
dc.identifier.scopusauthoridLi, T=36072008200en_US
dc.identifier.scopusauthoridHu, X=7404709241en_US
dc.identifier.scopusauthoridLiu, X=7409286408en_US
dc.identifier.scopusauthoridMurray, RM=35406239400en_US
dc.identifier.scopusauthoridSham, PC=34573429300en_US
dc.identifier.scopusauthoridAsherson, P=35402700900en_US
dc.identifier.scopusauthoridHill, L=7202617092en_US
dc.identifier.scopusauthoridNinomiya, T=14634502300en_US
dc.identifier.scopusauthoridCraig, I=7102548208en_US
dc.identifier.scopusauthoridOwen, M=36044041500en_US
dc.identifier.scopusauthoridPlomin, R=36050187200en_US
dc.identifier.scopusauthoridBreen, G=15742166000en_US
dc.identifier.scopusauthoridShaw, DJ=7403341741en_US
dc.identifier.scopusauthoridSt Clair, D=35354078200en_US

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