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Article: Characterization of model bile using fluorescence energy transfer from dehydroergosterol to dansylated lecithin

TitleCharacterization of model bile using fluorescence energy transfer from dehydroergosterol to dansylated lecithin
Authors
Issue Date2001
PublisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jlr.org/
Citation
Journal Of Lipid Research, 2001, v. 42 n. 6, p. 923-934 How to Cite?
AbstractFluorescence energy transfer from dehydroergosterol (DHE) to dansylated lecithin (DL) was used to characterize lecithin-cholesterol vesicles in the presence of the bile salt, sodium taurocholate. At lipid concentrations approximating physiological levels, exposure of fluorescently labeled vesicles to the bile salt led to a dose-dependent increase in the DHE-to-DL fluorescence ratio during the first 24 h after mixing. The initial changes in the fluorescence ratio correlated well with conventional turbidity measurements that quantify partial micellization of vesicles as a function of bile salt loading. In addition, fluorescence energy transfer from DHE to DL revealed cholesterol enrichment of vesicles and re-vesiculation of micelles at bile salt loadings for which vesicles and micelles coexisted. Samples containing the cholesterol-enriched vesicle fraction exhibited further increases in the DHE-to-DL fluorescence ratio during a 4-week observation period but only after a significant lag period of several days. The lag period decreased with cholesterol loading, and the increase in the fluorescence ratio always preceded the appearance of microscopic, birefringent, either needlelike or platelike, cholesterol crystals, in samples that were initially supersaturated with cholesterol. Cholesterol crystals were not observed, and the fluorescence ratio did not increase, for any sample that was undersaturated with cholesterol. Taken together, these results suggest that the latter changes in fluorescence are the result of cholesterol nucleation. Fluorescence energy transfer from DHE to DL is therefore a promising technique for the characterization of model bile and, possibly, provides a direct measurement of cholesterol nucleation.
Persistent Identifierhttp://hdl.handle.net/10722/175837
ISSN
2015 Impact Factor: 4.368
2015 SCImago Journal Rankings: 2.529
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWrenn, SPen_US
dc.contributor.authorGudheti, Men_US
dc.contributor.authorVeleva, ANen_US
dc.contributor.authorKaler, EWen_US
dc.contributor.authorLee, SPen_US
dc.date.accessioned2012-11-26T09:01:41Z-
dc.date.available2012-11-26T09:01:41Z-
dc.date.issued2001en_US
dc.identifier.citationJournal Of Lipid Research, 2001, v. 42 n. 6, p. 923-934en_US
dc.identifier.issn0022-2275en_US
dc.identifier.urihttp://hdl.handle.net/10722/175837-
dc.description.abstractFluorescence energy transfer from dehydroergosterol (DHE) to dansylated lecithin (DL) was used to characterize lecithin-cholesterol vesicles in the presence of the bile salt, sodium taurocholate. At lipid concentrations approximating physiological levels, exposure of fluorescently labeled vesicles to the bile salt led to a dose-dependent increase in the DHE-to-DL fluorescence ratio during the first 24 h after mixing. The initial changes in the fluorescence ratio correlated well with conventional turbidity measurements that quantify partial micellization of vesicles as a function of bile salt loading. In addition, fluorescence energy transfer from DHE to DL revealed cholesterol enrichment of vesicles and re-vesiculation of micelles at bile salt loadings for which vesicles and micelles coexisted. Samples containing the cholesterol-enriched vesicle fraction exhibited further increases in the DHE-to-DL fluorescence ratio during a 4-week observation period but only after a significant lag period of several days. The lag period decreased with cholesterol loading, and the increase in the fluorescence ratio always preceded the appearance of microscopic, birefringent, either needlelike or platelike, cholesterol crystals, in samples that were initially supersaturated with cholesterol. Cholesterol crystals were not observed, and the fluorescence ratio did not increase, for any sample that was undersaturated with cholesterol. Taken together, these results suggest that the latter changes in fluorescence are the result of cholesterol nucleation. Fluorescence energy transfer from DHE to DL is therefore a promising technique for the characterization of model bile and, possibly, provides a direct measurement of cholesterol nucleation.en_US
dc.languageengen_US
dc.publisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jlr.org/en_US
dc.relation.ispartofJournal of Lipid Researchen_US
dc.subject.meshBile - Metabolismen_US
dc.subject.meshCell Nucleus - Metabolismen_US
dc.subject.meshCholesterol - Metabolismen_US
dc.subject.meshChromatography, Gelen_US
dc.subject.meshDansyl Compounds - Metabolismen_US
dc.subject.meshDose-Response Relationship, Drugen_US
dc.subject.meshEgg Yolk - Chemistryen_US
dc.subject.meshErgosterol - Analogs & Derivatives - Metabolismen_US
dc.subject.meshLighten_US
dc.subject.meshNephelometry And Turbidimetryen_US
dc.subject.meshPhosphatidylcholines - Chemistryen_US
dc.subject.meshScattering, Radiationen_US
dc.subject.meshSpectrometry, Fluorescenceen_US
dc.subject.meshTaurocholic Acid - Pharmacologyen_US
dc.subject.meshTime Factorsen_US
dc.titleCharacterization of model bile using fluorescence energy transfer from dehydroergosterol to dansylated lecithinen_US
dc.typeArticleen_US
dc.identifier.emailLee, SP: sumlee@hku.hken_US
dc.identifier.authorityLee, SP=rp01351en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid11369800-
dc.identifier.scopuseid_2-s2.0-0034972962en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034972962&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume42en_US
dc.identifier.issue6en_US
dc.identifier.spage923en_US
dc.identifier.epage934en_US
dc.identifier.isiWOS:000169033200006-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridWrenn, SP=6603940041en_US
dc.identifier.scopusauthoridGudheti, M=16024338300en_US
dc.identifier.scopusauthoridVeleva, AN=12143837300en_US
dc.identifier.scopusauthoridKaler, EW=7007157989en_US
dc.identifier.scopusauthoridLee, SP=7601417497en_US

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