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Article: Mechanism of bile salt-induced mucin secretion by cultured dog gallbladder epithelial cells

TitleMechanism of bile salt-induced mucin secretion by cultured dog gallbladder epithelial cells
Authors
Issue Date1996
PublisherPortland Press Ltd. The Journal's web site is located at http://www.biochemj.org
Citation
Biochemical Journal, 1996, v. 316 n. 3, p. 873-877 How to Cite?
Abstract1. Hypersecretion of gallbladder mucin has been proposed to be a pathogenic factor in cholesterol gallstone formation. Using cultured gallbladder epithelial cells, we demonstrated that bile salts regulate mucin secretion by the gallbladder epithelium. In the present study we have investigated whether established second messenger pathways are involved in bile salt-induced mucin secretion. 2. The effect of activators and inhibitors on mucin secretion was studied by measuring the secretion of [3H]N-acetyl-D-glucosamine-labelled glycoproteins, Intracellular cAMP content of the cells was measured using a radioimmunoassay. 3. Incubation of the cells with 10 mM taurocholate did not increase the intracellular cAMP content (25.7 versus control 22.8 pmol of cAMP/mg of protein). No stimulation of mucin secretion was observed after incubation with 1-100 μM concentrations of the calcium ionophores ionomycin and A23187. The stimulatory effect of 10 mM tauroursodeoxycholate (TUDC) on mucin secretion could not be inhibited by the addition of EDTA. Activation of protein kinase C (PKC) by 1 μg/ml phorbol 12-myristate 13-acetate (PMA) caused an increase in mucin secretion (342% versus control 100%), comparable with the effect of 40 mM TUDC. The effect of 10 ng/ml PMA could partially be inhibited by a concentration of 2 μM of the PKC inhibitor staurosporin. Staurosporin had no inhibitory effect on mucin secretion induced by TUDC. 4. In gallbladder epithelial cells bile salts do not stimulate mucin secretion via one of the classical signal transduction pathways. We hypothesize that bile salts act on mucin secretion via a direct interaction with the apical membrane.
Persistent Identifierhttp://hdl.handle.net/10722/175743
ISSN
2015 Impact Factor: 3.562
2015 SCImago Journal Rankings: 2.582
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorKlinkspoor, JHen_US
dc.contributor.authorTytgat, GNJen_US
dc.contributor.authorLee, SPen_US
dc.contributor.authorGroen, AKen_US
dc.date.accessioned2012-11-26T09:00:53Z-
dc.date.available2012-11-26T09:00:53Z-
dc.date.issued1996en_US
dc.identifier.citationBiochemical Journal, 1996, v. 316 n. 3, p. 873-877en_US
dc.identifier.issn0264-6021en_US
dc.identifier.urihttp://hdl.handle.net/10722/175743-
dc.description.abstract1. Hypersecretion of gallbladder mucin has been proposed to be a pathogenic factor in cholesterol gallstone formation. Using cultured gallbladder epithelial cells, we demonstrated that bile salts regulate mucin secretion by the gallbladder epithelium. In the present study we have investigated whether established second messenger pathways are involved in bile salt-induced mucin secretion. 2. The effect of activators and inhibitors on mucin secretion was studied by measuring the secretion of [3H]N-acetyl-D-glucosamine-labelled glycoproteins, Intracellular cAMP content of the cells was measured using a radioimmunoassay. 3. Incubation of the cells with 10 mM taurocholate did not increase the intracellular cAMP content (25.7 versus control 22.8 pmol of cAMP/mg of protein). No stimulation of mucin secretion was observed after incubation with 1-100 μM concentrations of the calcium ionophores ionomycin and A23187. The stimulatory effect of 10 mM tauroursodeoxycholate (TUDC) on mucin secretion could not be inhibited by the addition of EDTA. Activation of protein kinase C (PKC) by 1 μg/ml phorbol 12-myristate 13-acetate (PMA) caused an increase in mucin secretion (342% versus control 100%), comparable with the effect of 40 mM TUDC. The effect of 10 ng/ml PMA could partially be inhibited by a concentration of 2 μM of the PKC inhibitor staurosporin. Staurosporin had no inhibitory effect on mucin secretion induced by TUDC. 4. In gallbladder epithelial cells bile salts do not stimulate mucin secretion via one of the classical signal transduction pathways. We hypothesize that bile salts act on mucin secretion via a direct interaction with the apical membrane.en_US
dc.languageengen_US
dc.publisherPortland Press Ltd. The Journal's web site is located at http://www.biochemj.orgen_US
dc.relation.ispartofBiochemical Journalen_US
dc.subject.meshAcetylglucosamine - Metabolismen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBile Acids And Salts - Pharmacologyen_US
dc.subject.meshCalcimycin - Pharmacologyen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshCyclic Amp - Metabolismen_US
dc.subject.meshDinoprostone - Pharmacologyen_US
dc.subject.meshDogsen_US
dc.subject.meshEdetic Acid - Pharmacologyen_US
dc.subject.meshEnzyme Activationen_US
dc.subject.meshEpithelium - Drug Effects - Physiologyen_US
dc.subject.meshGallbladder - Drug Effects - Physiologyen_US
dc.subject.meshIonomycin - Pharmacologyen_US
dc.subject.meshKineticsen_US
dc.subject.meshMucins - Biosynthesis - Secretionen_US
dc.subject.meshPhorbol 12,13-Dibutyrate - Pharmacologyen_US
dc.subject.meshProtein Kinase C - Metabolismen_US
dc.subject.meshSecond Messenger Systemsen_US
dc.subject.meshTaurochenodeoxycholic Acid - Pharmacologyen_US
dc.subject.meshTaurocholic Acid - Pharmacologyen_US
dc.subject.meshTetradecanoylphorbol Acetate - Pharmacologyen_US
dc.subject.meshTritiumen_US
dc.titleMechanism of bile salt-induced mucin secretion by cultured dog gallbladder epithelial cellsen_US
dc.typeArticleen_US
dc.identifier.emailLee, SP: sumlee@hku.hken_US
dc.identifier.authorityLee, SP=rp01351en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid8670165-
dc.identifier.scopuseid_2-s2.0-0029800445en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0029800445&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume316en_US
dc.identifier.issue3en_US
dc.identifier.spage873en_US
dc.identifier.epage877en_US
dc.identifier.isiWOS:A1996UV48300026-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridKlinkspoor, JH=6602590656en_US
dc.identifier.scopusauthoridTytgat, GNJ=35966168300en_US
dc.identifier.scopusauthoridLee, SP=7601417497en_US
dc.identifier.scopusauthoridGroen, AK=35242574800en_US

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