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Article: A constituent of human small intestinal mucus that copurifies with mucin and can interfere with raising antibodies to the mucin

TitleA constituent of human small intestinal mucus that copurifies with mucin and can interfere with raising antibodies to the mucin
Authors
Issue Date1994
PublisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/15216543.asp
Citation
Biochemistry And Molecular Biology International, 1994, v. 32 n. 6, p. 995-1002 How to Cite?
AbstractThe accuracy of an immunoassay for mucin depends on the antibodies' specificity. During human small intestinal mucin purification on a CsCl gradient, a very antigenic non-mucin contaminant was found at 1.48 g.ml -1 density. This was separated from the mucin (1.42 g.ml -1 density) by dividing the gradient into 26 fractions, but not by dividing it into 8 fractions. Polyclonal antibodies raised against mucin obtained using 8 fractions reacted with mucin and contaminant, but antibodies raised against mucin obtained using 26 fractions reacted only with mucin. The identity of the contaminant is unknown. However, it contained nearly equal amounts of galactose, N-acetylglucosamine and glucose, but did not react strongly with periodate-Schiff reagent. Immunofluorescence microscopy showed it was located in the small intestinal goblet cells and mucus layer. Unless the contaminant is removed before raising polyclonal antibodies to mucin, immunoassays will give inaccurate results.
Persistent Identifierhttp://hdl.handle.net/10722/175708
ISSN
2001 Impact Factor: 0.925
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorRabel, Ben_US
dc.contributor.authorCornish, Jen_US
dc.contributor.authorLee, SPen_US
dc.contributor.authorHarris, PJen_US
dc.contributor.authorTasmanJones, Cen_US
dc.contributor.authorRoberton, AMen_US
dc.date.accessioned2012-11-26T09:00:38Z-
dc.date.available2012-11-26T09:00:38Z-
dc.date.issued1994en_US
dc.identifier.citationBiochemistry And Molecular Biology International, 1994, v. 32 n. 6, p. 995-1002en_US
dc.identifier.issn1039-9712en_US
dc.identifier.urihttp://hdl.handle.net/10722/175708-
dc.description.abstractThe accuracy of an immunoassay for mucin depends on the antibodies' specificity. During human small intestinal mucin purification on a CsCl gradient, a very antigenic non-mucin contaminant was found at 1.48 g.ml -1 density. This was separated from the mucin (1.42 g.ml -1 density) by dividing the gradient into 26 fractions, but not by dividing it into 8 fractions. Polyclonal antibodies raised against mucin obtained using 8 fractions reacted with mucin and contaminant, but antibodies raised against mucin obtained using 26 fractions reacted only with mucin. The identity of the contaminant is unknown. However, it contained nearly equal amounts of galactose, N-acetylglucosamine and glucose, but did not react strongly with periodate-Schiff reagent. Immunofluorescence microscopy showed it was located in the small intestinal goblet cells and mucus layer. Unless the contaminant is removed before raising polyclonal antibodies to mucin, immunoassays will give inaccurate results.en_US
dc.languageengen_US
dc.publisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/15216543.aspen_US
dc.relation.ispartofBiochemistry and Molecular Biology Internationalen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibody Formationen_US
dc.subject.meshAntibody Specificityen_US
dc.subject.meshCentrifugation, Density Gradienten_US
dc.subject.meshChromatography, Gelen_US
dc.subject.meshChromatography, Thin Layeren_US
dc.subject.meshElectrophoresis, Polyacrylamide Gelen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_US
dc.subject.meshHumansen_US
dc.subject.meshIleum - Chemistryen_US
dc.subject.meshMicroscopy, Fluorescenceen_US
dc.subject.meshMucins - Immunology - Isolation & Purificationen_US
dc.subject.meshMucus - Chemistryen_US
dc.subject.meshPrecipitin Testsen_US
dc.subject.meshRabbitsen_US
dc.titleA constituent of human small intestinal mucus that copurifies with mucin and can interfere with raising antibodies to the mucinen_US
dc.typeArticleen_US
dc.identifier.emailLee, SP: sumlee@hku.hken_US
dc.identifier.authorityLee, SP=rp01351en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid8061635-
dc.identifier.scopuseid_2-s2.0-0028360230en_US
dc.identifier.volume32en_US
dc.identifier.issue6en_US
dc.identifier.spage995en_US
dc.identifier.epage1002en_US
dc.identifier.isiWOS:A1994NM26200001-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridRabel, B=16158653200en_US
dc.identifier.scopusauthoridCornish, J=7102230484en_US
dc.identifier.scopusauthoridLee, SP=7601417497en_US
dc.identifier.scopusauthoridHarris, PJ=35512009000en_US
dc.identifier.scopusauthoridTasmanJones, C=7003303326en_US
dc.identifier.scopusauthoridRoberton, AM=7003310534en_US

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