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Article: Use of the ileal conduit as a model for studying human small intestinal mucus glycoprotein secretion

TitleUse of the ileal conduit as a model for studying human small intestinal mucus glycoprotein secretion
Authors
Issue Date1991
PublisherAmerican Physiological Society. The Journal's web site is located at http://ajpcon.physiology.org/
Citation
American Journal Of Physiology - Gastrointestinal And Liver Physiology, 1991, v. 261 n. 5 24-5, p. G728-G734 How to Cite?
AbstractTo establish a human model system for investigating control of mucus secretion by the small intestine, we have studied the output of mucus glycoprotein in the urine of patients with ileal conduits. A highly specific enzyme-linked immunosorbent assay (ELISA) was used to measure mucus glycoprotein with a sensitivity down to 0.3 ng protein of mucus glycoprotein. The assay detects human ileal mucus glycoprotein in the urine of ileal conduit patients; for an individual subject, the amount hardly varied from day to day, although there were substantial differences between individuals in the amounts secreted. Control urine from persons with normal bladders did not contain any mucus glycoprotein that reacted in the ELISA. The model has many advantages over other experimental systems for studying the rate of mucus glycoprotein secretion from the human ileum and can also be used as a reproducible resource of undegraded mucus glycoprotein.
Persistent Identifierhttp://hdl.handle.net/10722/175668
ISSN
1998 Impact Factor: 3.077
2004 SCImago Journal Rankings: 1.102
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorRoberton, AMen_US
dc.contributor.authorRabel, Ben_US
dc.contributor.authorHarding, CAen_US
dc.contributor.authorTasmanJones, Cen_US
dc.contributor.authorHarris, PJen_US
dc.contributor.authorLee, SPen_US
dc.date.accessioned2012-11-26T09:00:22Z-
dc.date.available2012-11-26T09:00:22Z-
dc.date.issued1991en_US
dc.identifier.citationAmerican Journal Of Physiology - Gastrointestinal And Liver Physiology, 1991, v. 261 n. 5 24-5, p. G728-G734en_US
dc.identifier.issn0002-9513en_US
dc.identifier.urihttp://hdl.handle.net/10722/175668-
dc.description.abstractTo establish a human model system for investigating control of mucus secretion by the small intestine, we have studied the output of mucus glycoprotein in the urine of patients with ileal conduits. A highly specific enzyme-linked immunosorbent assay (ELISA) was used to measure mucus glycoprotein with a sensitivity down to 0.3 ng protein of mucus glycoprotein. The assay detects human ileal mucus glycoprotein in the urine of ileal conduit patients; for an individual subject, the amount hardly varied from day to day, although there were substantial differences between individuals in the amounts secreted. Control urine from persons with normal bladders did not contain any mucus glycoprotein that reacted in the ELISA. The model has many advantages over other experimental systems for studying the rate of mucus glycoprotein secretion from the human ileum and can also be used as a reproducible resource of undegraded mucus glycoprotein.en_US
dc.languageengen_US
dc.publisherAmerican Physiological Society. The Journal's web site is located at http://ajpcon.physiology.org/en_US
dc.relation.ispartofAmerican Journal of Physiology - Gastrointestinal and Liver Physiologyen_US
dc.subject.meshAmino Acids - Analysisen_US
dc.subject.meshAnastomosis, Surgicalen_US
dc.subject.meshCadaveren_US
dc.subject.meshElectrophoresis, Polyacrylamide Gelen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_US
dc.subject.meshGlycoproteins - Chemistry - Secretion - Urineen_US
dc.subject.meshHumansen_US
dc.subject.meshIleum - Secretionen_US
dc.subject.meshImmunodiffusionen_US
dc.subject.meshIntestine, Small - Secretionen_US
dc.subject.meshMonosaccharides - Analysisen_US
dc.subject.meshSensitivity And Specificityen_US
dc.subject.meshUrinary Diversionen_US
dc.titleUse of the ileal conduit as a model for studying human small intestinal mucus glycoprotein secretionen_US
dc.typeArticleen_US
dc.identifier.emailLee, SP: sumlee@hku.hken_US
dc.identifier.authorityLee, SP=rp01351en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid1951697-
dc.identifier.scopuseid_2-s2.0-0026322078en_US
dc.identifier.volume261en_US
dc.identifier.issue5 24-5en_US
dc.identifier.spageG728en_US
dc.identifier.epageG734en_US
dc.identifier.isiWOS:A1991GQ78000050-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridRoberton, AM=7003310534en_US
dc.identifier.scopusauthoridRabel, B=16158653200en_US
dc.identifier.scopusauthoridHarding, CA=7102457548en_US
dc.identifier.scopusauthoridTasmanJones, C=7003303326en_US
dc.identifier.scopusauthoridHarris, PJ=35512009000en_US
dc.identifier.scopusauthoridLee, SP=7601417497en_US

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