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- Publisher Website: 10.1016/0016-5085(85)90648-1
- Scopus: eid_2-s2.0-0022412832
- PMID: 2996964
- WOS: WOS:A1985AVB1700014
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Article: Pig gastric mucus: A one-way barrier for H+
Title | Pig gastric mucus: A one-way barrier for H+ |
---|---|
Authors | |
Issue Date | 1985 |
Publisher | WB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/gastro |
Citation | Gastroenterology, 1985, v. 89 n. 6, p. 1313-1318 How to Cite? |
Abstract | Gastric mucus is thought to protect the underlying mucosal cells from mechanical hazards and back-diffusion of luminal H+. In health, a pH gradient exists across the mucus layer from the variable low pH of the lumen to a pH approaching neutrality at the epithelial cell surface. By current hypotheses this gradient is maintained by the combined effects of an unstirred layer, restricted or slowed diffusion of H+ in the mucus, and the epithelial cell secretion of bicarbonate, which is confined to the cell surface by the mucus layer. These mechanisms do not explain how H+ is secreted through mucus in the first place. Using a modified diffusion chamber we have shown that pig gastric mucus facilitates a low-efficiency Na+/H+ exchange - a property that helps to clarify some previously unexplained components of H+ secretion. When a solution containing Na+ was separated by a layer of fresh pig gastric mucus from a solution of similar pH containing a much lower concentration of sodium, the sodium-rich solution was electrically negative relative to the sodium-poor solution and its pH decreased significantly with time. A similar pH gradient developed when the barrier was a synthetic cation-exchange membrane, and one of opposite sign when it was an anion exchanger; no pH gradient developed across neutral barriers. It is suggested that similar electrical coupling of H+ diffusion to active Na+ transport might in vivo ensure that secreted H+ moves into the gastric lumen. |
Persistent Identifier | http://hdl.handle.net/10722/175638 |
ISSN | 2021 Impact Factor: 33.883 2020 SCImago Journal Rankings: 7.828 |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Smith, GW | en_US |
dc.contributor.author | TasmanJones, C | en_US |
dc.contributor.author | Wiggins, PM | en_US |
dc.contributor.author | Lee, SP | en_US |
dc.date.accessioned | 2012-11-26T09:00:13Z | - |
dc.date.available | 2012-11-26T09:00:13Z | - |
dc.date.issued | 1985 | en_US |
dc.identifier.citation | Gastroenterology, 1985, v. 89 n. 6, p. 1313-1318 | en_US |
dc.identifier.issn | 0016-5085 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/175638 | - |
dc.description.abstract | Gastric mucus is thought to protect the underlying mucosal cells from mechanical hazards and back-diffusion of luminal H+. In health, a pH gradient exists across the mucus layer from the variable low pH of the lumen to a pH approaching neutrality at the epithelial cell surface. By current hypotheses this gradient is maintained by the combined effects of an unstirred layer, restricted or slowed diffusion of H+ in the mucus, and the epithelial cell secretion of bicarbonate, which is confined to the cell surface by the mucus layer. These mechanisms do not explain how H+ is secreted through mucus in the first place. Using a modified diffusion chamber we have shown that pig gastric mucus facilitates a low-efficiency Na+/H+ exchange - a property that helps to clarify some previously unexplained components of H+ secretion. When a solution containing Na+ was separated by a layer of fresh pig gastric mucus from a solution of similar pH containing a much lower concentration of sodium, the sodium-rich solution was electrically negative relative to the sodium-poor solution and its pH decreased significantly with time. A similar pH gradient developed when the barrier was a synthetic cation-exchange membrane, and one of opposite sign when it was an anion exchanger; no pH gradient developed across neutral barriers. It is suggested that similar electrical coupling of H+ diffusion to active Na+ transport might in vivo ensure that secreted H+ moves into the gastric lumen. | en_US |
dc.language | eng | en_US |
dc.publisher | WB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/gastro | en_US |
dc.relation.ispartof | Gastroenterology | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Biological Transport, Active | en_US |
dc.subject.mesh | Dialysis | en_US |
dc.subject.mesh | Diffusion | en_US |
dc.subject.mesh | Gastric Mucosa - Physiology | en_US |
dc.subject.mesh | Hydrogen-Ion Concentration | en_US |
dc.subject.mesh | Membrane Potentials | en_US |
dc.subject.mesh | Membranes, Artificial | en_US |
dc.subject.mesh | Mucus - Physiology | en_US |
dc.subject.mesh | Protons | en_US |
dc.subject.mesh | Sodium - Metabolism | en_US |
dc.subject.mesh | Swine | en_US |
dc.title | Pig gastric mucus: A one-way barrier for H+ | en_US |
dc.type | Article | en_US |
dc.identifier.email | Lee, SP: sumlee@hku.hk | en_US |
dc.identifier.authority | Lee, SP=rp01351 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1016/0016-5085(85)90648-1 | - |
dc.identifier.pmid | 2996964 | - |
dc.identifier.scopus | eid_2-s2.0-0022412832 | en_US |
dc.identifier.volume | 89 | en_US |
dc.identifier.issue | 6 | en_US |
dc.identifier.spage | 1313 | en_US |
dc.identifier.epage | 1318 | en_US |
dc.identifier.isi | WOS:A1985AVB1700014 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Smith, GW=7406739744 | en_US |
dc.identifier.scopusauthorid | TasmanJones, C=7003303326 | en_US |
dc.identifier.scopusauthorid | Wiggins, PM=7006787396 | en_US |
dc.identifier.scopusauthorid | Lee, SP=7601417497 | en_US |
dc.identifier.issnl | 0016-5085 | - |