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Article: Heterogeneity in gastrointestinal mucins

TitleHeterogeneity in gastrointestinal mucins
Authors
Issue Date1983
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bbagen
Citation
Biochimica Et Biophysica Acta - General Subjects, 1983, v. 760 n. 2, p. 262-269 How to Cite?
AbstractPig digestive tract mucins have often been used as model mucins for studying mucin structure, function and metabolism. In the present study pig gastric mucin and pig colonic mucin in the subunit form have been characterised and compared. Following Sepharose 4B or 2B-CL gel chromatography, the mucin eluant fractions were assayed colorimetrically by both the periodic acid-Schiff and the Alcian blue binding assays. Subunit colonic mucin eluted as a single unimodel peak that was easily detected by both assays. In contrast, subunit gastric mucin gave a peak primarily detected by periodic acid-Schiff that was overlapped by, but partially separated from, another peak primarily detected by Alcian blue. Subunit gastric mucin was separated into two periodic acid-Schiff staining spots when electrophoresed on cellulose acetate. Cetylpyridinium chloride (CPC) was able to precipitate only about half the subunit gastric mucin. The CPC-precipitable subunit gastric mucin corresponded to the faster running spot on electrophoresis, and the subunit gastric mucin in the CPC supernatant (which may have more than one subunit mucin type) to the slower spot(s). The former had a higher sulphate content and stained with Alcian blue. The latter had a lower sulphate content and showed very little Alcian blue reactivity. These results indicate that subunit pig gastric mucin is heterogeneous with respect to both size and charge. The differences between the types may be important in biological and physiochemical behaviour of gastric mucin. It seems likely that different laboratories may have worked on one or other of the pig gastric mucin types or a mixture, depending on the preparation method.
Persistent Identifierhttp://hdl.handle.net/10722/175631
ISSN
2015 Impact Factor: 5.083
2015 SCImago Journal Rankings: 2.128
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorStanley, RAen_US
dc.contributor.authorLee, SPen_US
dc.contributor.authorRoberton, AMen_US
dc.date.accessioned2012-11-26T09:00:12Z-
dc.date.available2012-11-26T09:00:12Z-
dc.date.issued1983en_US
dc.identifier.citationBiochimica Et Biophysica Acta - General Subjects, 1983, v. 760 n. 2, p. 262-269en_US
dc.identifier.issn0304-4165en_US
dc.identifier.urihttp://hdl.handle.net/10722/175631-
dc.description.abstractPig digestive tract mucins have often been used as model mucins for studying mucin structure, function and metabolism. In the present study pig gastric mucin and pig colonic mucin in the subunit form have been characterised and compared. Following Sepharose 4B or 2B-CL gel chromatography, the mucin eluant fractions were assayed colorimetrically by both the periodic acid-Schiff and the Alcian blue binding assays. Subunit colonic mucin eluted as a single unimodel peak that was easily detected by both assays. In contrast, subunit gastric mucin gave a peak primarily detected by periodic acid-Schiff that was overlapped by, but partially separated from, another peak primarily detected by Alcian blue. Subunit gastric mucin was separated into two periodic acid-Schiff staining spots when electrophoresed on cellulose acetate. Cetylpyridinium chloride (CPC) was able to precipitate only about half the subunit gastric mucin. The CPC-precipitable subunit gastric mucin corresponded to the faster running spot on electrophoresis, and the subunit gastric mucin in the CPC supernatant (which may have more than one subunit mucin type) to the slower spot(s). The former had a higher sulphate content and stained with Alcian blue. The latter had a lower sulphate content and showed very little Alcian blue reactivity. These results indicate that subunit pig gastric mucin is heterogeneous with respect to both size and charge. The differences between the types may be important in biological and physiochemical behaviour of gastric mucin. It seems likely that different laboratories may have worked on one or other of the pig gastric mucin types or a mixture, depending on the preparation method.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bbagenen_US
dc.relation.ispartofBiochimica et Biophysica Acta - General Subjectsen_US
dc.subject.meshAnimalsen_US
dc.subject.meshColon - Analysisen_US
dc.subject.meshElectrophoresis, Cellulose Acetateen_US
dc.subject.meshGastric Mucosa - Analysisen_US
dc.subject.meshIndicators And Reagentsen_US
dc.subject.meshIntestinal Mucosa - Analysisen_US
dc.subject.meshMucins - Isolation & Purificationen_US
dc.subject.meshSwineen_US
dc.titleHeterogeneity in gastrointestinal mucinsen_US
dc.typeArticleen_US
dc.identifier.emailLee, SP: sumlee@hku.hken_US
dc.identifier.authorityLee, SP=rp01351en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0304-4165(83)90172-1en_US
dc.identifier.pmid6626574-
dc.identifier.scopuseid_2-s2.0-0021091386en_US
dc.identifier.volume760en_US
dc.identifier.issue2en_US
dc.identifier.spage262en_US
dc.identifier.epage269en_US
dc.identifier.isiWOS:A1983RP54700009-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridStanley, RA=7201941695en_US
dc.identifier.scopusauthoridLee, SP=7601417497en_US
dc.identifier.scopusauthoridRoberton, AM=7003310534en_US

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