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Postgraduate Thesis: Fabrication of multi-component tissue for intervertebral disc tissue engineering
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TitleFabrication of multi-component tissue for intervertebral disc tissue engineering
 
AuthorsChik, Tsz-kit.
戚子傑.
 
Issue Date2012
 
PublisherThe University of Hong Kong (Pokfulam, Hong Kong)
 
AbstractIntervertebral disc tissue engineering is challenging because it involves the integration of multiple tissues with distinct structures and compositions such as lamellar annulus fibrosus, gel?like nucleus pulposus and cartilage endplate. Each of them has different compositions and different structures. It is hypothesized that integration of tissues can be enhanced with appropriate mechanical and biological stimuli. Meanwhile, effect of torsional stimulus on cell re?orientation in mesenchymal stem cell?collagen tubular constructs is investigated in this study. Furthermore, it is proposed that these findings can be used to fabricate a multicomponent unit for intervertebral disc tissue engineering. It has been demonstrated that mechanical and biological stimuli can stabilize the interface between osteogenic and chondrogenic differentiated constructs with enhanced ultimate tensile stress while the phenotype of osteogenic and chondrogenic differentiated constructs were maintained. Scanning electronic microscopic images have shown aligned collagen fibrils and presence of calcium at the interface, indicating the possibility of the formation of a calcified zone. In addition, it is proven that torsional stimulus triggered re?orientation of mesenchymal stem cells in collagen lamellae towards a preferred angle. Cell alignments were confirmed by using a MatLab?based program to analyze the actin filament and the cell alignment via Phalloidin and Hematoxylin staining, respectively. Cells and actin filaments were inclined around 30o from the vertical axis, while cells and filaments in the control group (static loading) aligned along the vertical axis. Furthermore, a double?layers bioengineered unit was fabricated, with intact osteogenic differentiated parts at both ends. Comparatively higher cell density was observed at the interface between layers, demonstrating the interactions between layers, while the phenotype of each part was maintained in 14 days culture. This study concludes that a multi?components bioengineered unit with preferred cell alignments can be fabricated. This provides new insights to future development of bioengineered spinal motion segment for treating late stage disc degeneration.
 
AdvisorsChan, BP
Sze, KY
 
DegreeDoctor of Philosophy
 
SubjectIntervertebral disk prostheses.
Tissue engineering - Materials.
 
Dept/ProgramMechanical Engineering
 
DC FieldValue
dc.contributor.advisorChan, BP
 
dc.contributor.advisorSze, KY
 
dc.contributor.authorChik, Tsz-kit.
 
dc.contributor.author戚子傑.
 
dc.date.hkucongregation2012
 
dc.date.issued2012
 
dc.description.abstractIntervertebral disc tissue engineering is challenging because it involves the integration of multiple tissues with distinct structures and compositions such as lamellar annulus fibrosus, gel?like nucleus pulposus and cartilage endplate. Each of them has different compositions and different structures. It is hypothesized that integration of tissues can be enhanced with appropriate mechanical and biological stimuli. Meanwhile, effect of torsional stimulus on cell re?orientation in mesenchymal stem cell?collagen tubular constructs is investigated in this study. Furthermore, it is proposed that these findings can be used to fabricate a multicomponent unit for intervertebral disc tissue engineering. It has been demonstrated that mechanical and biological stimuli can stabilize the interface between osteogenic and chondrogenic differentiated constructs with enhanced ultimate tensile stress while the phenotype of osteogenic and chondrogenic differentiated constructs were maintained. Scanning electronic microscopic images have shown aligned collagen fibrils and presence of calcium at the interface, indicating the possibility of the formation of a calcified zone. In addition, it is proven that torsional stimulus triggered re?orientation of mesenchymal stem cells in collagen lamellae towards a preferred angle. Cell alignments were confirmed by using a MatLab?based program to analyze the actin filament and the cell alignment via Phalloidin and Hematoxylin staining, respectively. Cells and actin filaments were inclined around 30o from the vertical axis, while cells and filaments in the control group (static loading) aligned along the vertical axis. Furthermore, a double?layers bioengineered unit was fabricated, with intact osteogenic differentiated parts at both ends. Comparatively higher cell density was observed at the interface between layers, demonstrating the interactions between layers, while the phenotype of each part was maintained in 14 days culture. This study concludes that a multi?components bioengineered unit with preferred cell alignments can be fabricated. This provides new insights to future development of bioengineered spinal motion segment for treating late stage disc degeneration.
 
dc.description.naturepublished_or_final_version
 
dc.description.thesisdisciplineMechanical Engineering
 
dc.description.thesisleveldoctoral
 
dc.description.thesisnameDoctor of Philosophy
 
dc.identifier.hkulb4784944
 
dc.languageeng
 
dc.publisherThe University of Hong Kong (Pokfulam, Hong Kong)
 
dc.relation.ispartofHKU Theses Online (HKUTO)
 
dc.rightsThe author retains all proprietary rights, (such as patent rights) and the right to use in future works.
 
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
 
dc.source.urihttp://hub.hku.hk/bib/B47849447
 
dc.subject.lcshIntervertebral disk prostheses.
 
dc.subject.lcshTissue engineering - Materials.
 
dc.titleFabrication of multi-component tissue for intervertebral disc tissue engineering
 
dc.typePG_Thesis
 
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<item><contributor.advisor>Chan, BP</contributor.advisor>
<contributor.advisor>Sze, KY</contributor.advisor>
<contributor.author>Chik, Tsz-kit.</contributor.author>
<contributor.author>&#25114;&#23376;&#20625;.</contributor.author>
<date.issued>2012</date.issued>
<description.abstract>&#65279;Intervertebral disc tissue engineering is challenging because it involves the

integration of multiple tissues with distinct structures and compositions such as

lamellar annulus fibrosus, gel?like nucleus pulposus and cartilage endplate. Each

of them has different compositions and different structures. It is hypothesized

that integration of tissues can be enhanced with appropriate mechanical and

biological stimuli. Meanwhile, effect of torsional stimulus on cell re?orientation

in mesenchymal stem cell?collagen tubular constructs is investigated in this study.

Furthermore, it is proposed that these findings can be used to fabricate a multicomponent

unit for intervertebral disc tissue engineering. It has been

demonstrated that mechanical and biological stimuli can stabilize the interface

between osteogenic and chondrogenic differentiated constructs with enhanced

ultimate tensile stress while the phenotype of osteogenic and chondrogenic

differentiated constructs were maintained. Scanning electronic microscopic

images have shown aligned collagen fibrils and presence of calcium at the

interface, indicating the possibility of the formation of a calcified zone. In

addition, it is proven that torsional stimulus triggered re?orientation of

mesenchymal stem cells in collagen lamellae towards a preferred angle. Cell

alignments were confirmed by using a MatLab?based program to analyze the

actin filament and the cell alignment via Phalloidin and Hematoxylin staining,

respectively. Cells and actin filaments were inclined around 30o from the vertical

axis, while cells and filaments in the control group (static loading) aligned along

the vertical axis. Furthermore, a double?layers bioengineered unit was fabricated,

with intact osteogenic differentiated parts at both ends. Comparatively higher

cell density was observed at the interface between layers, demonstrating the

interactions between layers, while the phenotype of each part was maintained in

14 days culture. This study concludes that a multi?components bioengineered

unit with preferred cell alignments can be fabricated. This provides new insights

to future development of bioengineered spinal motion segment for treating late

stage disc degeneration.</description.abstract>
<language>eng</language>
<publisher>The University of Hong Kong (Pokfulam, Hong Kong)</publisher>
<relation.ispartof>HKU Theses Online (HKUTO)</relation.ispartof>
<rights>The author retains all proprietary rights, (such as patent rights) and the right to use in future works.</rights>
<rights>Creative Commons: Attribution 3.0 Hong Kong License</rights>
<source.uri>http://hub.hku.hk/bib/B47849447</source.uri>
<subject.lcsh>Intervertebral disk prostheses.</subject.lcsh>
<subject.lcsh>Tissue engineering - Materials.</subject.lcsh>
<title>Fabrication of multi-component tissue for intervertebral disc tissue engineering</title>
<type>PG_Thesis</type>
<identifier.hkul>b4784944</identifier.hkul>
<description.thesisname>Doctor of Philosophy</description.thesisname>
<description.thesislevel>doctoral</description.thesislevel>
<description.thesisdiscipline>Mechanical Engineering</description.thesisdiscipline>
<description.nature>published_or_final_version</description.nature>
<date.hkucongregation>2012</date.hkucongregation>
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