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Postgraduate Thesis: Molecular characterization of different subgenomic regions of hepatitis C virus genotype 6 in Hong Kong
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TitleMolecular characterization of different subgenomic regions of hepatitis C virus genotype 6 in Hong Kong
 
AuthorsLi, Miu-shan.
李妙珊.
 
Issue Date2012
 
PublisherThe University of Hong Kong (Pokfulam, Hong Kong)
 
AbstractHepatitis C Virus “HCV” is the major factor to develop the chronic liver disease. Accurate genotyping is important to decide the choice and duration of therapy. The most common method for the determination of the genotype in HCV is the direct sequencing of the 5’UTR region, CORE, CORE/E1 and NS5B region. The current study is to compare different subgenomic regions for the molecular characterization of genotype 6 in Hong Kong and study the molecular epidemiology by phylogenetic analysis. Ninety-four patients were included in the study from 2006 to 2009. There was no discordant result between different subgenomic regions. The percentage of the patients sequenced from CORE, CORE/E1 and NS5B were 95%, 93% and 78% respectively. All the HCV strains were genotype 6a except two patients were other subtypes of genotype 6. The phylogenetic analysis in the neighbor-joining tree in CORE/E1 and NS5B region can clearly discriminate between the subtypes of genotype 6, however it cannot show in the NJ tree of the CORE region sequences. It is to conclude that CORE/E1 is the best choice both for the characterization of genotype 6 and used for phylogenetic analysis in the study.
 
DegreeMaster of Medical Sciences
 
SubjectHepatitis C virus - Genetics.
 
Dept/ProgramMicrobiology
 
DC FieldValue
dc.contributor.authorLi, Miu-shan.
 
dc.contributor.author李妙珊.
 
dc.date.hkucongregation2012
 
dc.date.issued2012
 
dc.description.abstractHepatitis C Virus “HCV” is the major factor to develop the chronic liver disease. Accurate genotyping is important to decide the choice and duration of therapy. The most common method for the determination of the genotype in HCV is the direct sequencing of the 5’UTR region, CORE, CORE/E1 and NS5B region. The current study is to compare different subgenomic regions for the molecular characterization of genotype 6 in Hong Kong and study the molecular epidemiology by phylogenetic analysis. Ninety-four patients were included in the study from 2006 to 2009. There was no discordant result between different subgenomic regions. The percentage of the patients sequenced from CORE, CORE/E1 and NS5B were 95%, 93% and 78% respectively. All the HCV strains were genotype 6a except two patients were other subtypes of genotype 6. The phylogenetic analysis in the neighbor-joining tree in CORE/E1 and NS5B region can clearly discriminate between the subtypes of genotype 6, however it cannot show in the NJ tree of the CORE region sequences. It is to conclude that CORE/E1 is the best choice both for the characterization of genotype 6 and used for phylogenetic analysis in the study.
 
dc.description.naturepublished_or_final_version
 
dc.description.thesisdisciplineMicrobiology
 
dc.description.thesislevelmaster's
 
dc.description.thesisnameMaster of Medical Sciences
 
dc.identifier.hkulb4833416
 
dc.languageeng
 
dc.publisherThe University of Hong Kong (Pokfulam, Hong Kong)
 
dc.relation.ispartofHKU Theses Online (HKUTO)
 
dc.rightsThe author retains all proprietary rights, (such as patent rights) and the right to use in future works.
 
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
 
dc.source.urihttp://hub.hku.hk/bib/B48334169
 
dc.subject.lcshHepatitis C virus - Genetics.
 
dc.titleMolecular characterization of different subgenomic regions of hepatitis C virus genotype 6 in Hong Kong
 
dc.typePG_Thesis
 
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