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Postgraduate Thesis: Molecular characterization of clostridium difficile isolates by capillary gel electrophoresis-based PCR ribotyping
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TitleMolecular characterization of clostridium difficile isolates by capillary gel electrophoresis-based PCR ribotyping
 
AuthorsLam, Ching-to.
林正道.
 
Issue Date2012
 
PublisherThe University of Hong Kong (Pokfulam, Hong Kong)
 
AbstractBackgroud: Clostridium difficile infection (CDI) is a global concern since the emergence of the hyper-virulent strain NAP1/BI/027. The incidence of CDI has been increasing and active surveillance of Clostridium difficile epidemiology is of paramount importance. In studying the epidemiology of CDI, PCR ribotyping is a very useful method in finding the genetic relatedness of outbreak and epidemic strains. Characterisation of Clostridium difficile isolates can help surveillance and active monitoring, hence reducing the chance of potential outbreaks. Aim: The aims of this study is to investigate the epidemiology of Clostridium difficile in Hong Kong in the year 2010, and that of a Clostridium difficile outbreak in 2011 by using capillary gel electrophoresis-based PCR ribotyping. Results: Among the 307 toxigenic isolates, 139 isolates (45.3%) were characterized as existing ribotypes. Ribotype 012 was the predominant ribotype (17.3%), followed by ribotype 002/0 (15.6%). A total of 144 isolates (46.9%) were characterized as new ribotypes. The remaining 24 isolates (7.8%) were characterized as of high resemblance of existing ribotypes. A total of 8/12 isolates (66.7%) of the outbreak strains were found to be ribotype 002/0. Conclusion: The predominant strains of Clostridium difficile in 2010 was ribotype 012. The 2011 outbreak in Kowloon Hospital was an outbreak of Clostridium difficile ribotype 002/0. Capillary gel electrophoresis-based PCR ribotyping was showed to be a useful tool in investigation of Clostridium difficile outbreaks and study of Clostridium difficile epidemiology.
 
DegreeMaster of Medical Sciences
 
SubjectClostridium difficile - Molecular aspects.
 
Dept/ProgramMicrobiology
 
DC FieldValue
dc.contributor.authorLam, Ching-to.
 
dc.contributor.author林正道.
 
dc.date.hkucongregation2012
 
dc.date.issued2012
 
dc.description.abstractBackgroud: Clostridium difficile infection (CDI) is a global concern since the emergence of the hyper-virulent strain NAP1/BI/027. The incidence of CDI has been increasing and active surveillance of Clostridium difficile epidemiology is of paramount importance. In studying the epidemiology of CDI, PCR ribotyping is a very useful method in finding the genetic relatedness of outbreak and epidemic strains. Characterisation of Clostridium difficile isolates can help surveillance and active monitoring, hence reducing the chance of potential outbreaks. Aim: The aims of this study is to investigate the epidemiology of Clostridium difficile in Hong Kong in the year 2010, and that of a Clostridium difficile outbreak in 2011 by using capillary gel electrophoresis-based PCR ribotyping. Results: Among the 307 toxigenic isolates, 139 isolates (45.3%) were characterized as existing ribotypes. Ribotype 012 was the predominant ribotype (17.3%), followed by ribotype 002/0 (15.6%). A total of 144 isolates (46.9%) were characterized as new ribotypes. The remaining 24 isolates (7.8%) were characterized as of high resemblance of existing ribotypes. A total of 8/12 isolates (66.7%) of the outbreak strains were found to be ribotype 002/0. Conclusion: The predominant strains of Clostridium difficile in 2010 was ribotype 012. The 2011 outbreak in Kowloon Hospital was an outbreak of Clostridium difficile ribotype 002/0. Capillary gel electrophoresis-based PCR ribotyping was showed to be a useful tool in investigation of Clostridium difficile outbreaks and study of Clostridium difficile epidemiology.
 
dc.description.naturepublished_or_final_version
 
dc.description.thesisdisciplineMicrobiology
 
dc.description.thesislevelmaster's
 
dc.description.thesisnameMaster of Medical Sciences
 
dc.identifier.hkulb4833399
 
dc.languageeng
 
dc.publisherThe University of Hong Kong (Pokfulam, Hong Kong)
 
dc.relation.ispartofHKU Theses Online (HKUTO)
 
dc.rightsThe author retains all proprietary rights, (such as patent rights) and the right to use in future works.
 
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
 
dc.source.urihttp://hub.hku.hk/bib/B48333992
 
dc.subject.lcshClostridium difficile - Molecular aspects.
 
dc.titleMolecular characterization of clostridium difficile isolates by capillary gel electrophoresis-based PCR ribotyping
 
dc.typePG_Thesis
 
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<date.issued>2012</date.issued>
<description.abstract>&#65279;Backgroud:

Clostridium difficile infection (CDI) is a global concern since the emergence of the hyper-virulent strain NAP1/BI/027. The incidence of CDI has been increasing and active surveillance of Clostridium difficile epidemiology is of paramount importance. In studying the epidemiology of CDI, PCR ribotyping is a very useful method in finding the genetic relatedness of outbreak and epidemic strains. Characterisation of Clostridium difficile isolates can help surveillance and active monitoring, hence reducing the chance of potential outbreaks.



Aim:

The aims of this study is to investigate the epidemiology of Clostridium difficile in Hong Kong in the year 2010, and that of a Clostridium difficile outbreak in 2011 by using capillary gel electrophoresis-based PCR ribotyping.



Results:

Among the 307 toxigenic isolates, 139 isolates (45.3%) were characterized as existing ribotypes. Ribotype 012 was the predominant ribotype (17.3%), followed by ribotype 002/0 (15.6%). A total of 144 isolates (46.9%) were characterized as new ribotypes. The remaining 24 isolates (7.8%) were characterized as of high resemblance of existing ribotypes. A total of 8/12 isolates (66.7%) of the outbreak strains were found to be ribotype 002/0.



Conclusion:

The predominant strains of Clostridium difficile in 2010 was ribotype 012. The 2011 outbreak in Kowloon Hospital was an outbreak of Clostridium difficile ribotype 002/0. Capillary gel electrophoresis-based PCR ribotyping was showed to be a useful tool in investigation of Clostridium difficile outbreaks and study of Clostridium difficile epidemiology.</description.abstract>
<language>eng</language>
<publisher>The University of Hong Kong (Pokfulam, Hong Kong)</publisher>
<relation.ispartof>HKU Theses Online (HKUTO)</relation.ispartof>
<rights>The author retains all proprietary rights, (such as patent rights) and the right to use in future works.</rights>
<rights>Creative Commons: Attribution 3.0 Hong Kong License</rights>
<source.uri>http://hub.hku.hk/bib/B48333992</source.uri>
<subject.lcsh>Clostridium difficile - Molecular aspects.</subject.lcsh>
<title>Molecular characterization of clostridium difficile isolates by capillary gel electrophoresis-based PCR ribotyping</title>
<type>PG_Thesis</type>
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<description.thesisname>Master of Medical Sciences</description.thesisname>
<description.thesislevel>master&apos;s</description.thesislevel>
<description.thesisdiscipline>Microbiology</description.thesisdiscipline>
<description.nature>published_or_final_version</description.nature>
<date.hkucongregation>2012</date.hkucongregation>
<bitstream.url>http://hub.hku.hk/bitstream/10722/173847/1/FullText.pdf</bitstream.url>
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