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Conference Paper: Nanomechanical properties of vimentin intermediate filament

TitleNanomechanical properties of vimentin intermediate filament
Authors
KeywordsAfm
Atomic Force Microscopy
Force Spectroscopy
Intermediate Filament
Nanomechanics
Nanotechnology
Vimentin
Issue Date2012
PublisherTrans Tech Publications Ltd.. The Journal's web site is located at http://www.scitec.ch/1022-6680/
Citation
Advanced Materials Research, 2012, v. 463-464, p. 748-752 How to Cite?
Abstract
Vimentin intermediate filament (IF) is one of the major proteins which built the cytoskeleton network alongside with the microtubule and actin filament. Though it was known that the vimentin IF network plays an important role in the mechanical behaviours of cells, it is surprised that its mechanical behaviours are not fully understood to date. The aim of this paper is to study the nanomechanical properties of vimentin IF using the atomic force spectroscopy (AFM) which allows the manipulation and force spectroscopy of filaments. The vimentin intermediate filaments were attached to the APTES (3- aminopropyltriethoxy) functionalized mica which offered better adhesive force. In the force spectroscopy study, the AFM tip was allowed to clamp filaments and then retraced. The force-displacement curve of the process was obtained for analysis. The curves can be grouped into two major groups - sewtooth and plateau. The appearance of sewtooth was more frequent than the plateau. The sudden force changes (jumps from higher to lower force) in sewtooth and plateau curves were also analyzed. It was shown that the partial ruptures which denoted by the jumps favoured small force (∼100 pN) and short range (separation of jumps below 25 nm). This result also demonstrated the probability of different modes of partial IF ruptures. © (2012) Trans Tech Publications.
Persistent Identifierhttp://hdl.handle.net/10722/173424
ISSN
2013 SCImago Journal Rankings: 0.144
ISI Accession Number ID
References

 

Author Affiliations
  1. The University of Hong Kong
DC FieldValueLanguage
dc.contributor.authorWong, Ken_US
dc.contributor.authorAhw, Nen_US
dc.contributor.authorDky, Sen_US
dc.contributor.authorLu, Wen_US
dc.date.accessioned2012-10-30T06:31:02Z-
dc.date.available2012-10-30T06:31:02Z-
dc.date.issued2012en_US
dc.identifier.citationAdvanced Materials Research, 2012, v. 463-464, p. 748-752en_US
dc.identifier.issn1022-6680en_US
dc.identifier.urihttp://hdl.handle.net/10722/173424-
dc.description.abstractVimentin intermediate filament (IF) is one of the major proteins which built the cytoskeleton network alongside with the microtubule and actin filament. Though it was known that the vimentin IF network plays an important role in the mechanical behaviours of cells, it is surprised that its mechanical behaviours are not fully understood to date. The aim of this paper is to study the nanomechanical properties of vimentin IF using the atomic force spectroscopy (AFM) which allows the manipulation and force spectroscopy of filaments. The vimentin intermediate filaments were attached to the APTES (3- aminopropyltriethoxy) functionalized mica which offered better adhesive force. In the force spectroscopy study, the AFM tip was allowed to clamp filaments and then retraced. The force-displacement curve of the process was obtained for analysis. The curves can be grouped into two major groups - sewtooth and plateau. The appearance of sewtooth was more frequent than the plateau. The sudden force changes (jumps from higher to lower force) in sewtooth and plateau curves were also analyzed. It was shown that the partial ruptures which denoted by the jumps favoured small force (∼100 pN) and short range (separation of jumps below 25 nm). This result also demonstrated the probability of different modes of partial IF ruptures. © (2012) Trans Tech Publications.en_US
dc.languageengen_US
dc.publisherTrans Tech Publications Ltd.. The Journal's web site is located at http://www.scitec.ch/1022-6680/en_US
dc.relation.ispartofAdvanced Materials Researchen_US
dc.subjectAfmen_US
dc.subjectAtomic Force Microscopyen_US
dc.subjectForce Spectroscopyen_US
dc.subjectIntermediate Filamenten_US
dc.subjectNanomechanicsen_US
dc.subjectNanotechnologyen_US
dc.subjectVimentinen_US
dc.titleNanomechanical properties of vimentin intermediate filamenten_US
dc.typeConference_Paperen_US
dc.identifier.emailLu, W:wwlu@hku.hken_US
dc.identifier.authorityLu, W=rp00411en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.4028/www.scientific.net/AMR.463-464.748en_US
dc.identifier.scopuseid_2-s2.0-84857306355en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84857306355&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume463-464en_US
dc.identifier.spage748en_US
dc.identifier.epage752en_US
dc.identifier.isiWOS:000308114100144-
dc.publisher.placeSwitzerlanden_US
dc.identifier.scopusauthoridWong, K=24460004600en_US
dc.identifier.scopusauthoridAhw, N=55014688200en_US
dc.identifier.scopusauthoridDky, S=55014688300en_US
dc.identifier.scopusauthoridLu, W=7404215221en_US

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