Conference Paper: Nanomechanical properties of vimentin intermediate filament

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TitleNanomechanical properties of vimentin intermediate filament
AuthorsWong, K1
Ahw, N1
Dky, S1
Lu, W1
KeywordsAfm
Atomic Force Microscopy
Force Spectroscopy
Intermediate Filament
Nanomechanics
Nanotechnology
Vimentin
Issue Date2012
PublisherTrans Tech Publications Ltd.. The Journal's web site is located at http://www.scitec.ch/1022-6680/
CitationAdvanced Materials Research, 2012, v. 463-464, p. 748-752 [How to Cite?]
DOI: http://dx.doi.org/10.4028/www.scientific.net/AMR.463-464.748
AbstractVimentin intermediate filament (IF) is one of the major proteins which built the cytoskeleton network alongside with the microtubule and actin filament. Though it was known that the vimentin IF network plays an important role in the mechanical behaviours of cells, it is surprised that its mechanical behaviours are not fully understood to date. The aim of this paper is to study the nanomechanical properties of vimentin IF using the atomic force spectroscopy (AFM) which allows the manipulation and force spectroscopy of filaments. The vimentin intermediate filaments were attached to the APTES (3- aminopropyltriethoxy) functionalized mica which offered better adhesive force. In the force spectroscopy study, the AFM tip was allowed to clamp filaments and then retraced. The force-displacement curve of the process was obtained for analysis. The curves can be grouped into two major groups - sewtooth and plateau. The appearance of sewtooth was more frequent than the plateau. The sudden force changes (jumps from higher to lower force) in sewtooth and plateau curves were also analyzed. It was shown that the partial ruptures which denoted by the jumps favoured small force (∼100 pN) and short range (separation of jumps below 25 nm). This result also demonstrated the probability of different modes of partial IF ruptures. © (2012) Trans Tech Publications.
ISSN1022-6680
2011 SCImago Journal Rankings: 0.030
DOIhttp://dx.doi.org/10.4028/www.scientific.net/AMR.463-464.748
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorWong, K
dc.contributor.authorAhw, N
dc.contributor.authorDky, S
dc.contributor.authorLu, W
dc.date.accessioned2012-10-30T06:31:02Z
dc.date.available2012-10-30T06:31:02Z
dc.date.issued2012
dc.description.abstractVimentin intermediate filament (IF) is one of the major proteins which built the cytoskeleton network alongside with the microtubule and actin filament. Though it was known that the vimentin IF network plays an important role in the mechanical behaviours of cells, it is surprised that its mechanical behaviours are not fully understood to date. The aim of this paper is to study the nanomechanical properties of vimentin IF using the atomic force spectroscopy (AFM) which allows the manipulation and force spectroscopy of filaments. The vimentin intermediate filaments were attached to the APTES (3- aminopropyltriethoxy) functionalized mica which offered better adhesive force. In the force spectroscopy study, the AFM tip was allowed to clamp filaments and then retraced. The force-displacement curve of the process was obtained for analysis. The curves can be grouped into two major groups - sewtooth and plateau. The appearance of sewtooth was more frequent than the plateau. The sudden force changes (jumps from higher to lower force) in sewtooth and plateau curves were also analyzed. It was shown that the partial ruptures which denoted by the jumps favoured small force (∼100 pN) and short range (separation of jumps below 25 nm). This result also demonstrated the probability of different modes of partial IF ruptures. © (2012) Trans Tech Publications.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationAdvanced Materials Research, 2012, v. 463-464, p. 748-752 [How to Cite?]
DOI: http://dx.doi.org/10.4028/www.scientific.net/AMR.463-464.748
dc.identifier.doihttp://dx.doi.org/10.4028/www.scientific.net/AMR.463-464.748
dc.identifier.epage752
dc.identifier.issn1022-6680
2011 SCImago Journal Rankings: 0.030
dc.identifier.scopuseid_2-s2.0-84857306355
dc.identifier.spage748
dc.identifier.urihttp://hdl.handle.net/10722/173424
dc.identifier.volume463-464
dc.languageeng
dc.publisherTrans Tech Publications Ltd.. The Journal's web site is located at http://www.scitec.ch/1022-6680/
dc.publisher.placeSwitzerland
dc.relation.ispartofAdvanced Materials Research
dc.relation.referencesReferences in Scopus
dc.subjectAfm
dc.subjectAtomic Force Microscopy
dc.subjectForce Spectroscopy
dc.subjectIntermediate Filament
dc.subjectNanomechanics
dc.subjectNanotechnology
dc.subjectVimentin
dc.titleNanomechanical properties of vimentin intermediate filament
dc.typeConference_Paper
Author Affiliations
  1. The University of Hong Kong