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Conference Paper: Nanomechanical properties of vimentin intermediate filament
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TitleNanomechanical properties of vimentin intermediate filament
 
AuthorsWong, K1
Ahw, N1
Dky, S1
Lu, W1
 
KeywordsAfm
Atomic Force Microscopy
Force Spectroscopy
Intermediate Filament
Nanomechanics
Nanotechnology
Vimentin
 
Issue Date2012
 
PublisherTrans Tech Publications Ltd.. The Journal's web site is located at http://www.scitec.ch/1022-6680/
 
CitationAdvanced Materials Research, 2012, v. 463-464, p. 748-752 [How to Cite?]
DOI: http://dx.doi.org/10.4028/www.scientific.net/AMR.463-464.748
 
AbstractVimentin intermediate filament (IF) is one of the major proteins which built the cytoskeleton network alongside with the microtubule and actin filament. Though it was known that the vimentin IF network plays an important role in the mechanical behaviours of cells, it is surprised that its mechanical behaviours are not fully understood to date. The aim of this paper is to study the nanomechanical properties of vimentin IF using the atomic force spectroscopy (AFM) which allows the manipulation and force spectroscopy of filaments. The vimentin intermediate filaments were attached to the APTES (3- aminopropyltriethoxy) functionalized mica which offered better adhesive force. In the force spectroscopy study, the AFM tip was allowed to clamp filaments and then retraced. The force-displacement curve of the process was obtained for analysis. The curves can be grouped into two major groups - sewtooth and plateau. The appearance of sewtooth was more frequent than the plateau. The sudden force changes (jumps from higher to lower force) in sewtooth and plateau curves were also analyzed. It was shown that the partial ruptures which denoted by the jumps favoured small force (∼100 pN) and short range (separation of jumps below 25 nm). This result also demonstrated the probability of different modes of partial IF ruptures. © (2012) Trans Tech Publications.
 
ISSN1022-6680
2012 SCImago Journal Rankings: 0.134
 
DOIhttp://dx.doi.org/10.4028/www.scientific.net/AMR.463-464.748
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorWong, K
 
dc.contributor.authorAhw, N
 
dc.contributor.authorDky, S
 
dc.contributor.authorLu, W
 
dc.date.accessioned2012-10-30T06:31:02Z
 
dc.date.available2012-10-30T06:31:02Z
 
dc.date.issued2012
 
dc.description.abstractVimentin intermediate filament (IF) is one of the major proteins which built the cytoskeleton network alongside with the microtubule and actin filament. Though it was known that the vimentin IF network plays an important role in the mechanical behaviours of cells, it is surprised that its mechanical behaviours are not fully understood to date. The aim of this paper is to study the nanomechanical properties of vimentin IF using the atomic force spectroscopy (AFM) which allows the manipulation and force spectroscopy of filaments. The vimentin intermediate filaments were attached to the APTES (3- aminopropyltriethoxy) functionalized mica which offered better adhesive force. In the force spectroscopy study, the AFM tip was allowed to clamp filaments and then retraced. The force-displacement curve of the process was obtained for analysis. The curves can be grouped into two major groups - sewtooth and plateau. The appearance of sewtooth was more frequent than the plateau. The sudden force changes (jumps from higher to lower force) in sewtooth and plateau curves were also analyzed. It was shown that the partial ruptures which denoted by the jumps favoured small force (∼100 pN) and short range (separation of jumps below 25 nm). This result also demonstrated the probability of different modes of partial IF ruptures. © (2012) Trans Tech Publications.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationAdvanced Materials Research, 2012, v. 463-464, p. 748-752 [How to Cite?]
DOI: http://dx.doi.org/10.4028/www.scientific.net/AMR.463-464.748
 
dc.identifier.doihttp://dx.doi.org/10.4028/www.scientific.net/AMR.463-464.748
 
dc.identifier.epage752
 
dc.identifier.issn1022-6680
2012 SCImago Journal Rankings: 0.134
 
dc.identifier.scopuseid_2-s2.0-84857306355
 
dc.identifier.spage748
 
dc.identifier.urihttp://hdl.handle.net/10722/173424
 
dc.identifier.volume463-464
 
dc.languageeng
 
dc.publisherTrans Tech Publications Ltd.. The Journal's web site is located at http://www.scitec.ch/1022-6680/
 
dc.publisher.placeSwitzerland
 
dc.relation.ispartofAdvanced Materials Research
 
dc.relation.referencesReferences in Scopus
 
dc.subjectAfm
 
dc.subjectAtomic Force Microscopy
 
dc.subjectForce Spectroscopy
 
dc.subjectIntermediate Filament
 
dc.subjectNanomechanics
 
dc.subjectNanotechnology
 
dc.subjectVimentin
 
dc.titleNanomechanical properties of vimentin intermediate filament
 
dc.typeConference_Paper
 
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Author Affiliations
  1. The University of Hong Kong