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Article: Glycodelin suppresses endometrial cell migration and invasion but stimulates spheroid attachment

TitleGlycodelin suppresses endometrial cell migration and invasion but stimulates spheroid attachment
Authors
KeywordsAttachment
Glycodelin
Invasion
Migration
Spheroid
Issue Date2012
PublisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/721149/description#description
Citation
Reproductive Biomedicine Online, 2012, v. 24 n. 6, p. 639-645 How to Cite?
AbstractGlycodelin contains four isoforms with diverse biological functions. Glycodelin-A is potentially a diagnostic marker for cancer patients and receptivity marker of the secretory endometrium. Yet, direct evidence for the role of glycodelin in the regulation of endometrial epithelial cell migration, invasion and attachment of trophoblastic spheroids (blastocyst surrogate) is lacking. In this study, the human glycodelin gene was stably transfected into human endometrial (HEC1-B) cells. Forced expression of glycodelin in HEC1-B cells did not affect cell proliferation, cell viability or cell-cycle progression, but significantly reduced migration and invasion of the stably transfected cells (both P < 0.05). The migration rate returned to normal levels when the glycodelin-forced-expressing HEC1-B cells were treated with glycodelin RNAi. Furthermore, forced expression of glycodelin in HEC1-B cells significantly increased the attachment of trophoblastic spheroids onto the endometrial epithelial cells (P < 0.05). In summary, glycodelin suppressed endometrial cell migration and invasion but enhanced spheroid attachment. © 2012, Reproductive Healthcare Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/173374
ISSN
2015 Impact Factor: 2.796
2015 SCImago Journal Rankings: 1.459
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSo, KHen_US
dc.contributor.authorLee, CLen_US
dc.contributor.authorYeung, WSBen_US
dc.contributor.authorLee, KFen_US
dc.date.accessioned2012-10-30T06:29:44Z-
dc.date.available2012-10-30T06:29:44Z-
dc.date.issued2012en_US
dc.identifier.citationReproductive Biomedicine Online, 2012, v. 24 n. 6, p. 639-645en_US
dc.identifier.issn1472-6483en_US
dc.identifier.urihttp://hdl.handle.net/10722/173374-
dc.description.abstractGlycodelin contains four isoforms with diverse biological functions. Glycodelin-A is potentially a diagnostic marker for cancer patients and receptivity marker of the secretory endometrium. Yet, direct evidence for the role of glycodelin in the regulation of endometrial epithelial cell migration, invasion and attachment of trophoblastic spheroids (blastocyst surrogate) is lacking. In this study, the human glycodelin gene was stably transfected into human endometrial (HEC1-B) cells. Forced expression of glycodelin in HEC1-B cells did not affect cell proliferation, cell viability or cell-cycle progression, but significantly reduced migration and invasion of the stably transfected cells (both P < 0.05). The migration rate returned to normal levels when the glycodelin-forced-expressing HEC1-B cells were treated with glycodelin RNAi. Furthermore, forced expression of glycodelin in HEC1-B cells significantly increased the attachment of trophoblastic spheroids onto the endometrial epithelial cells (P < 0.05). In summary, glycodelin suppressed endometrial cell migration and invasion but enhanced spheroid attachment. © 2012, Reproductive Healthcare Ltd.en_US
dc.languageengen_US
dc.publisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/721149/description#descriptionen_US
dc.relation.ispartofReproductive BioMedicine Onlineen_US
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Reproductive Biomedicine Online. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Reproductive Biomedicine Online, 2012, v. 24 n. 6, p. 639-645. DOI: 10.1016/j.rbmo.2012.03.004-
dc.subjectAttachmenten_US
dc.subjectGlycodelinen_US
dc.subjectInvasionen_US
dc.subjectMigrationen_US
dc.subjectSpheroiden_US
dc.titleGlycodelin suppresses endometrial cell migration and invasion but stimulates spheroid attachmenten_US
dc.typeArticleen_US
dc.identifier.emailLee, KF:ckflee@hku.hken_US
dc.identifier.authorityLee, KF=rp00458en_US
dc.description.naturepostprinten_US
dc.identifier.doi10.1016/j.rbmo.2012.03.004en_US
dc.identifier.pmid22503278-
dc.identifier.scopuseid_2-s2.0-84861823425en_US
dc.identifier.hkuros205914-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84861823425&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume24en_US
dc.identifier.issue6en_US
dc.identifier.spage639en_US
dc.identifier.epage645en_US
dc.identifier.isiWOS:000304806600010-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridSo, KH=55178410500en_US
dc.identifier.scopusauthoridLee, CL=52663638100en_US
dc.identifier.scopusauthoridYeung, WSB=54899819600en_US
dc.identifier.scopusauthoridLee, KF=26643097500en_US

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