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Article: Quantification of transforming growth factor β1 (TGFβ1) mRNA expression in mouse preimplantation embryos and determination of TGFβ receptor (type I and type II) expression in mouse embryos and reproductive tract

TitleQuantification of transforming growth factor β1 (TGFβ1) mRNA expression in mouse preimplantation embryos and determination of TGFβ receptor (type I and type II) expression in mouse embryos and reproductive tract
Authors
Issue Date2001
PublisherOxford University Press. The Journal's web site is located at http://molehr.oxfordjournals.org/
Citation
Molecular Human Reproduction, 2001, v. 7 n. 11, p. 1047-1056 How to Cite?
AbstractWe hypothesized that transforming growth factor β1 (TGFβ 1) and its receptors play a role in the interaction between the preimplantation embryo and the reproductive tract. To investigate this hypothesis, TGFβ 1 mRNA in mouse embryos was quantified by competitive reverse transcription-polymerase chain reaction using an RNA mimic. TGFβ 1 was first detected in the unfertilized oocyte, disappeared after fertilization and was expressed again at the 2-cell stage (4410 ± 1330 transcripts/embryo). Its expression increased gradually, peaked at the 8-cell stage (58 600 ± 17 300 transcripts/embryo) and declined rapidly after the morula stage reaching a concentration of 1520 ± 546 transcripts/embryo at the blastocyst stage. The mRNA levels of TGFβ 1 at the 8-cell and morula stages were significantly higher than that at other cell stages (P < 0.05). The expression of TGF receptors in embryos and in the reproductive tract was also investigated. Both TGFβ 1 type I (ALK-5) and type II TGFβ receptors were detected in embryos from 1-cell to blastocyst stage by immunohistochemistry. Northern hybridization and immunohistochemistry showed a constant expression of both TGFβ receptors in the oviduct from day 1 to day 4 of pregnancy, whilst in the uterus there was a marked increase in the expression of TGFβ type I receptor on day 3. Expression of TGFβ type II receptor in the uterus remained unaltered throughout the study period. This study has shown that preimplantation mouse embryos produce TGFβ 1 and that both the embryos and the reproductive tract are responsive to TGFβ 1 in the preimplantation period.
Persistent Identifierhttp://hdl.handle.net/10722/173249
ISSN
2015 Impact Factor: 3.943
2015 SCImago Journal Rankings: 1.611
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChow, JFCen_US
dc.contributor.authorLee, KFen_US
dc.contributor.authorChan, STHen_US
dc.contributor.authorYeung, WSBen_US
dc.date.accessioned2012-10-30T06:28:46Z-
dc.date.available2012-10-30T06:28:46Z-
dc.date.issued2001en_US
dc.identifier.citationMolecular Human Reproduction, 2001, v. 7 n. 11, p. 1047-1056en_US
dc.identifier.issn1360-9947en_US
dc.identifier.urihttp://hdl.handle.net/10722/173249-
dc.description.abstractWe hypothesized that transforming growth factor β1 (TGFβ 1) and its receptors play a role in the interaction between the preimplantation embryo and the reproductive tract. To investigate this hypothesis, TGFβ 1 mRNA in mouse embryos was quantified by competitive reverse transcription-polymerase chain reaction using an RNA mimic. TGFβ 1 was first detected in the unfertilized oocyte, disappeared after fertilization and was expressed again at the 2-cell stage (4410 ± 1330 transcripts/embryo). Its expression increased gradually, peaked at the 8-cell stage (58 600 ± 17 300 transcripts/embryo) and declined rapidly after the morula stage reaching a concentration of 1520 ± 546 transcripts/embryo at the blastocyst stage. The mRNA levels of TGFβ 1 at the 8-cell and morula stages were significantly higher than that at other cell stages (P < 0.05). The expression of TGF receptors in embryos and in the reproductive tract was also investigated. Both TGFβ 1 type I (ALK-5) and type II TGFβ receptors were detected in embryos from 1-cell to blastocyst stage by immunohistochemistry. Northern hybridization and immunohistochemistry showed a constant expression of both TGFβ receptors in the oviduct from day 1 to day 4 of pregnancy, whilst in the uterus there was a marked increase in the expression of TGFβ type I receptor on day 3. Expression of TGFβ type II receptor in the uterus remained unaltered throughout the study period. This study has shown that preimplantation mouse embryos produce TGFβ 1 and that both the embryos and the reproductive tract are responsive to TGFβ 1 in the preimplantation period.en_US
dc.languageengen_US
dc.publisherOxford University Press. The Journal's web site is located at http://molehr.oxfordjournals.org/en_US
dc.relation.ispartofMolecular Human Reproductionen_US
dc.rightsMolecular Human Reproduction. Copyright © Oxford University Press.-
dc.subject.meshActivin Receptors, Type I - Genetics - Metabolismen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBlastocyst - Physiologyen_US
dc.subject.meshBlotting, Northernen_US
dc.subject.meshFallopian Tubes - Physiologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshGene Expression Regulation, Developmentalen_US
dc.subject.meshGenitalia, Female - Physiologyen_US
dc.subject.meshMaleen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred Strainsen_US
dc.subject.meshProtein-Serine-Threonine Kinasesen_US
dc.subject.meshRna, Messenger - Metabolismen_US
dc.subject.meshReceptors, Transforming Growth Factor Beta - Genetics - Metabolismen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.subject.meshSensitivity And Specificityen_US
dc.subject.meshTransforming Growth Factor Beta - Geneticsen_US
dc.subject.meshTransforming Growth Factor Beta1en_US
dc.subject.meshUterus - Physiologyen_US
dc.titleQuantification of transforming growth factor β1 (TGFβ1) mRNA expression in mouse preimplantation embryos and determination of TGFβ receptor (type I and type II) expression in mouse embryos and reproductive tracten_US
dc.typeArticleen_US
dc.identifier.emailLee, KF:ckflee@hku.hken_US
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_US
dc.identifier.authorityLee, KF=rp00458en_US
dc.identifier.authorityYeung, WSB=rp00331en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1093/molehr/7.11.1047-
dc.identifier.pmid11675471-
dc.identifier.scopuseid_2-s2.0-0034753316en_US
dc.identifier.hkuros65368-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034753316&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume7en_US
dc.identifier.issue11en_US
dc.identifier.spage1047en_US
dc.identifier.epage1056en_US
dc.identifier.isiWOS:000172030400006-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridChow, JFC=7401728953en_US
dc.identifier.scopusauthoridLee, KF=26643097500en_US
dc.identifier.scopusauthoridChan, STH=24368283200en_US
dc.identifier.scopusauthoridYeung, WSB=7102370745en_US

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