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Article: Molecular cloning and expression analysis of human glycogen synthase kinase-3α promoter

TitleMolecular cloning and expression analysis of human glycogen synthase kinase-3α promoter
Authors
Issue Date2000
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/molbrainres
Citation
Molecular Brain Research, 2000, v. 84 n. 1-2, p. 150-157 How to Cite?
AbstractHuman glycogen synthase kinase-3α (GSK-3α) is a serine/threonine kinase that phosphorylates a variety of cytoplasmic and nuclear proteins. It also phosphorylates components of the neuronal cytoskeleton including tau and neurofilament heavy chain. Hyperphosphorylated tau is found in neurofibrillary tangles, a hallmark of Alzheimer's disease and aberrant phosphorylation of neurofilament heavy chain is observed in motor neuron disease. Alterations in GSK-3α activity may therefore contribute to the disease process in these disorders. As a first step to understand the transcriptional regulation of GSK-3α, a 2-kb (p-1751/+243) DNA fragment upstream of the GSK-3α initiation codon was obtained from a YAC clone and characterised. Using primer extension assays, a putative transcriptional start site was located to a G nucleotide 244 bp upstream of the ATG codon. Several transcription factor-binding sites were identified on the promoter region, but no TATA-like element was located close to the start site. Deletion mutants of the 2-kb DNA fragment were generated and fused to a promoterless chloramphenicol acetyltransferase (CAT) gene. Transfection study in a neuroblastoma cell line revealed the 1-kb (p-719/+243) fragment carried strong promoter activity, while the 2-kb construct that contains an Alu-like sequence was only 50% active. (C) 2000 Elsevier Science B.V.
Persistent Identifierhttp://hdl.handle.net/10722/173248
ISSN
2007 Impact Factor: 1.997
2008 SCImago Journal Rankings: 1.457
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLee, KFen_US
dc.contributor.authorChan, JYCen_US
dc.contributor.authorLau, KFen_US
dc.contributor.authorLee, WCen_US
dc.contributor.authorMiller, CCJen_US
dc.contributor.authorAnderton, BHen_US
dc.contributor.authorShaw, PCen_US
dc.date.accessioned2012-10-30T06:28:46Z-
dc.date.available2012-10-30T06:28:46Z-
dc.date.issued2000en_US
dc.identifier.citationMolecular Brain Research, 2000, v. 84 n. 1-2, p. 150-157en_US
dc.identifier.issn0169-328Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/173248-
dc.description.abstractHuman glycogen synthase kinase-3α (GSK-3α) is a serine/threonine kinase that phosphorylates a variety of cytoplasmic and nuclear proteins. It also phosphorylates components of the neuronal cytoskeleton including tau and neurofilament heavy chain. Hyperphosphorylated tau is found in neurofibrillary tangles, a hallmark of Alzheimer's disease and aberrant phosphorylation of neurofilament heavy chain is observed in motor neuron disease. Alterations in GSK-3α activity may therefore contribute to the disease process in these disorders. As a first step to understand the transcriptional regulation of GSK-3α, a 2-kb (p-1751/+243) DNA fragment upstream of the GSK-3α initiation codon was obtained from a YAC clone and characterised. Using primer extension assays, a putative transcriptional start site was located to a G nucleotide 244 bp upstream of the ATG codon. Several transcription factor-binding sites were identified on the promoter region, but no TATA-like element was located close to the start site. Deletion mutants of the 2-kb DNA fragment were generated and fused to a promoterless chloramphenicol acetyltransferase (CAT) gene. Transfection study in a neuroblastoma cell line revealed the 1-kb (p-719/+243) fragment carried strong promoter activity, while the 2-kb construct that contains an Alu-like sequence was only 50% active. (C) 2000 Elsevier Science B.V.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/molbrainresen_US
dc.relation.ispartofMolecular Brain Researchen_US
dc.rightsMolecular Brain Research. Copyright © Elsevier BV.-
dc.subject.meshAlu Elements - Geneticsen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshCalcium-Calmodulin-Dependent Protein Kinases - Geneticsen_US
dc.subject.meshChromosomes, Artificial, Yeast - Geneticsen_US
dc.subject.meshCloning, Molecularen_US
dc.subject.meshGene Expression Regulationen_US
dc.subject.meshGenes, Reporteren_US
dc.subject.meshGlycogen Synthase Kinase 3en_US
dc.subject.meshGlycogen Synthase Kinasesen_US
dc.subject.meshHumansen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshPromoter Regions, Genetic - Geneticsen_US
dc.subject.meshProtein Subunitsen_US
dc.subject.meshSequence Deletion - Geneticsen_US
dc.subject.meshTata Box - Geneticsen_US
dc.subject.meshTransfectionen_US
dc.subject.meshTumor Cells, Cultureden_US
dc.titleMolecular cloning and expression analysis of human glycogen synthase kinase-3α promoteren_US
dc.typeArticleen_US
dc.identifier.emailLee, KF:ckflee@hku.hken_US
dc.identifier.authorityLee, KF=rp00458en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/S0169-328X(00)00238-2en_US
dc.identifier.pmid11113543-
dc.identifier.scopuseid_2-s2.0-0034624204en_US
dc.identifier.hkuros56325-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034624204&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume84en_US
dc.identifier.issue1-2en_US
dc.identifier.spage150en_US
dc.identifier.epage157en_US
dc.identifier.isiWOS:000166095000018-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridLee, KF=26643097500en_US
dc.identifier.scopusauthoridChan, JYC=55230715100en_US
dc.identifier.scopusauthoridLau, KF=7401560031en_US
dc.identifier.scopusauthoridLee, WC=8557741900en_US
dc.identifier.scopusauthoridMiller, CCJ=8980202200en_US
dc.identifier.scopusauthoridAnderton, BH=7006709314en_US
dc.identifier.scopusauthoridShaw, PC=35599523600en_US

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