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Article: Changes in p21(WAF1), pRb, Mdm-2, Bax and Bcl-2 expression in cervical cancer cell lines transfected with a p53 expressing adenovirus

TitleChanges in p21(WAF1), pRb, Mdm-2, Bax and Bcl-2 expression in cervical cancer cell lines transfected with a p53 expressing adenovirus
Authors
KeywordsAdenoviral Vector
Bax/Bcl-2
Cervical Cancer Cell Lines
Growth Arrest/Apoptosis
Mdm2
P21(Waf1)
P53
Prb
Issue Date2000
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/ejca
Citation
European Journal Of Cancer, 2000, v. 36 n. 2, p. 249-256 How to Cite?
AbstractThe aim of this study was to provide some insights into the molecular mechanisms involved in p53-dependent apoptosis and growth arrest. Changes in the levels of p53 protein and proteins regulated by p53 were studied in relation to events of the cell cycle and apoptosis in cervical cancer cell lines upon transfection with a p53 expressing adenovirus (Ad5-p53). The post- transfection level of p53 protein in SiHa cells was found to be unchanged during the 24-48 h period. In contrast, the level of p21(WAF1) protein was shown to increase to its highest level at 24 h, and decreased gradually up to 48 h after the Ad5-p53 transfection. We further noted that the increase of p21(WAF1) was accompanied by G1 arrest at 24 h and the decrease of p21(WAF1) was associated with apoptosis at 36-48 h after transfection. An anti- p21(WAF1) antibody cross-reactive protein band of approximately 14 kDa was observed in HeLa and C-33A cells when these cells were committed to apoptosis upon Ad5-p53 transfection. In SiHa cells, phosphorylation of pRb was inhibited during the early stage of Ad5-p53 transfection. This was followed by the cleavage of pRb. However, Ad5-p53 transfection did not change the levels of Bax and Bcl-2 proteins. Our results suggested that, Bax and Bcl-2 may not be important for the apoptosis of these cells, whereas cleavage of Rb, and the decrease of p21(WAF1) could play important roles in p53-dependent apoptosis. (C) 2000 Elsevier Science Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/173236
ISSN
2015 Impact Factor: 6.163
2015 SCImago Journal Rankings: 3.152
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHuang, TGen_US
dc.contributor.authorIp, SMen_US
dc.contributor.authorYeung, WSBen_US
dc.contributor.authorNgan, HYSen_US
dc.date.accessioned2012-10-30T06:28:42Z-
dc.date.available2012-10-30T06:28:42Z-
dc.date.issued2000en_US
dc.identifier.citationEuropean Journal Of Cancer, 2000, v. 36 n. 2, p. 249-256en_US
dc.identifier.issn0959-8049en_US
dc.identifier.urihttp://hdl.handle.net/10722/173236-
dc.description.abstractThe aim of this study was to provide some insights into the molecular mechanisms involved in p53-dependent apoptosis and growth arrest. Changes in the levels of p53 protein and proteins regulated by p53 were studied in relation to events of the cell cycle and apoptosis in cervical cancer cell lines upon transfection with a p53 expressing adenovirus (Ad5-p53). The post- transfection level of p53 protein in SiHa cells was found to be unchanged during the 24-48 h period. In contrast, the level of p21(WAF1) protein was shown to increase to its highest level at 24 h, and decreased gradually up to 48 h after the Ad5-p53 transfection. We further noted that the increase of p21(WAF1) was accompanied by G1 arrest at 24 h and the decrease of p21(WAF1) was associated with apoptosis at 36-48 h after transfection. An anti- p21(WAF1) antibody cross-reactive protein band of approximately 14 kDa was observed in HeLa and C-33A cells when these cells were committed to apoptosis upon Ad5-p53 transfection. In SiHa cells, phosphorylation of pRb was inhibited during the early stage of Ad5-p53 transfection. This was followed by the cleavage of pRb. However, Ad5-p53 transfection did not change the levels of Bax and Bcl-2 proteins. Our results suggested that, Bax and Bcl-2 may not be important for the apoptosis of these cells, whereas cleavage of Rb, and the decrease of p21(WAF1) could play important roles in p53-dependent apoptosis. (C) 2000 Elsevier Science Ltd.en_US
dc.languageengen_US
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/ejcaen_US
dc.relation.ispartofEuropean Journal of Canceren_US
dc.subjectAdenoviral Vectoren_US
dc.subjectBax/Bcl-2en_US
dc.subjectCervical Cancer Cell Linesen_US
dc.subjectGrowth Arrest/Apoptosisen_US
dc.subjectMdm2en_US
dc.subjectP21(Waf1)en_US
dc.subjectP53en_US
dc.subjectPrben_US
dc.titleChanges in p21(WAF1), pRb, Mdm-2, Bax and Bcl-2 expression in cervical cancer cell lines transfected with a p53 expressing adenovirusen_US
dc.typeArticleen_US
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_US
dc.identifier.emailNgan, HYS:hysngan@hkucc.hku.hken_US
dc.identifier.authorityYeung, WSB=rp00331en_US
dc.identifier.authorityNgan, HYS=rp00346en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/S0959-8049(99)00247-6en_US
dc.identifier.pmid10741285-
dc.identifier.scopuseid_2-s2.0-0033621992en_US
dc.identifier.hkuros48867-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033621992&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume36en_US
dc.identifier.issue2en_US
dc.identifier.spage249en_US
dc.identifier.epage256en_US
dc.identifier.isiWOS:000085548400025-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridHuang, TG=7404962005en_US
dc.identifier.scopusauthoridIp, SM=55041321600en_US
dc.identifier.scopusauthoridYeung, WSB=7102370745en_US
dc.identifier.scopusauthoridNgan, HYS=34571944100en_US

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