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Article: Competitive immunoassay using protein g affinity capillary chromatography with laser induced fluorescence detection

TitleCompetitive immunoassay using protein g affinity capillary chromatography with laser induced fluorescence detection
Authors
Issue Date1999
PublisherTaylor & Francis Inc. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/10826076.asp
Citation
Journal Of Liquid Chromatography And Related Technologies, 1999, v. 22 n. 20, p. 3129-3137 How to Cite?
AbstractA simple approach to perform competitive immunoassay using protein G affinity capillary chromatography and laser induced fluorescence (LIF) detection was described. Bovine serum albumin (BSA) was used as a model to test the performance of the system. The assay used fluorescein isothiocyanate (FITC) labeled BSA as a tracer and an anti-BSA monoclonal antibody as the immunoreagent. Capillaries with inner diameter of 150 μm were packed with recombinant protein G bound perfusive support and used to capture the immunocomplexes, which were subsequently desorbed by 100 mM glycine (pH 9.0) and then detected using LIF. The results demonstrated the feasibility to perform competitive immunoassay using this system with high sensitivity and small sample size.
Persistent Identifierhttp://hdl.handle.net/10722/173229
ISSN
2015 Impact Factor: 0.669
2015 SCImago Journal Rankings: 0.299
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWang, Qen_US
dc.contributor.authorLuo, Gen_US
dc.contributor.authorYeung, WSBen_US
dc.date.accessioned2012-10-30T06:28:39Z-
dc.date.available2012-10-30T06:28:39Z-
dc.date.issued1999en_US
dc.identifier.citationJournal Of Liquid Chromatography And Related Technologies, 1999, v. 22 n. 20, p. 3129-3137en_US
dc.identifier.issn1082-6076en_US
dc.identifier.urihttp://hdl.handle.net/10722/173229-
dc.description.abstractA simple approach to perform competitive immunoassay using protein G affinity capillary chromatography and laser induced fluorescence (LIF) detection was described. Bovine serum albumin (BSA) was used as a model to test the performance of the system. The assay used fluorescein isothiocyanate (FITC) labeled BSA as a tracer and an anti-BSA monoclonal antibody as the immunoreagent. Capillaries with inner diameter of 150 μm were packed with recombinant protein G bound perfusive support and used to capture the immunocomplexes, which were subsequently desorbed by 100 mM glycine (pH 9.0) and then detected using LIF. The results demonstrated the feasibility to perform competitive immunoassay using this system with high sensitivity and small sample size.en_US
dc.languageengen_US
dc.publisherTaylor & Francis Inc. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/10826076.aspen_US
dc.relation.ispartofJournal of Liquid Chromatography and Related Technologiesen_US
dc.titleCompetitive immunoassay using protein g affinity capillary chromatography with laser induced fluorescence detectionen_US
dc.typeArticleen_US
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_US
dc.identifier.authorityYeung, WSB=rp00331en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1081/JLC-100102080en_US
dc.identifier.scopuseid_2-s2.0-0032741403en_US
dc.identifier.hkuros50293-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032741403&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume22en_US
dc.identifier.issue20en_US
dc.identifier.spage3129en_US
dc.identifier.epage3137en_US
dc.identifier.isiWOS:000084218300006-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridWang, Q=7406915351en_US
dc.identifier.scopusauthoridLuo, G=7401536462en_US
dc.identifier.scopusauthoridYeung, WSB=7102370745en_US

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