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Article: Predictive value of sperm morphology and movement characteristics in the outcome of in vitro fertilization of human oocytes

TitlePredictive value of sperm morphology and movement characteristics in the outcome of in vitro fertilization of human oocytes
Authors
Issue Date1989
Citation
Journal Of In Vitro Fertilization And Embryo Transfer, 1989, v. 6 n. 3, p. 142-148 How to Cite?
AbstractOne hundred fourteen semen samples from Chinese males were analyzed for routine semen parameters including the semen volume, sperm count, percentage motility, and percentage normal morphology. Of these 114 samples, 54 also had movement characteristics of seminal and swim-up sperm evaluated by the computer image analyzer system (Cellsoft; Cryo Resources Co., New York). All semen samples were subjected to the swim-up procedure to harvest the motile sperm before inseminations of human oocytes. Fertilization was considered to have occurred when at least one oocyte was observed with two or more pronuclei. Semen samples were classified as infertile (0% fertilization rate; N = 32) or fertile (>0% fertilization rate; N = 82) before statistical analyses. There was a significant difference (P < 0.005) in percentage normal morphology of seminal sperm between the fertile (mean ± SE; 67.3 ± 1.2%) and the infertile (59.3 ± 2.2%) samples. The percentage normal morphology of seminal sperm correlated (r = 0.3049; P < 0.002) with the fertilization rate and this parameter was selected by the multivariate stepwise discriminant analysis as the discriminator capable of predicting the fertilization rate with 57.9% accuracy. Statistical analyses of samples where sperm movement was also evaluated demonstrated that there was significant differences (P < 0.01) between the fertile (N = 38) and the infertile (N = 16) samples in percentage normal morphology of seminal sperm (67.8 ± 1.8% vs 56.2 ± 2.6%) and curvilinear velocity of swim-up sperm (89.2 ± 3.5 vs 68.2 ± 7.2 μ/sec.). The fertilization rates correlated with the percentage normal morphology of seminal sperm ( r = 0.3868; P < 0.005) and velocity of swim-up sperm (r = 0.3842; P < 0.005). Multivariate stepwise discriminant analysis demonstrated that these two sperm parameters in combination were capable of predicting the fertilization rate with 74.1% accuracy. Our results indicate that seminal sperm morphology, coupled with computer-assisted image analysis of movement characteristics of swim-up sperm, can help to predict the outcome of in vitro fertilization of human oocytes.
Persistent Identifierhttp://hdl.handle.net/10722/173146
ISSN
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChan, SYWen_US
dc.contributor.authorWang, Cen_US
dc.contributor.authorChan, STHen_US
dc.contributor.authorHo, PCen_US
dc.contributor.authorSo, WWKen_US
dc.contributor.authorChan, YFen_US
dc.contributor.authorMa, HKen_US
dc.date.accessioned2012-10-30T06:28:10Z-
dc.date.available2012-10-30T06:28:10Z-
dc.date.issued1989en_US
dc.identifier.citationJournal Of In Vitro Fertilization And Embryo Transfer, 1989, v. 6 n. 3, p. 142-148en_US
dc.identifier.issn0740-7769en_US
dc.identifier.urihttp://hdl.handle.net/10722/173146-
dc.description.abstractOne hundred fourteen semen samples from Chinese males were analyzed for routine semen parameters including the semen volume, sperm count, percentage motility, and percentage normal morphology. Of these 114 samples, 54 also had movement characteristics of seminal and swim-up sperm evaluated by the computer image analyzer system (Cellsoft; Cryo Resources Co., New York). All semen samples were subjected to the swim-up procedure to harvest the motile sperm before inseminations of human oocytes. Fertilization was considered to have occurred when at least one oocyte was observed with two or more pronuclei. Semen samples were classified as infertile (0% fertilization rate; N = 32) or fertile (>0% fertilization rate; N = 82) before statistical analyses. There was a significant difference (P < 0.005) in percentage normal morphology of seminal sperm between the fertile (mean ± SE; 67.3 ± 1.2%) and the infertile (59.3 ± 2.2%) samples. The percentage normal morphology of seminal sperm correlated (r = 0.3049; P < 0.002) with the fertilization rate and this parameter was selected by the multivariate stepwise discriminant analysis as the discriminator capable of predicting the fertilization rate with 57.9% accuracy. Statistical analyses of samples where sperm movement was also evaluated demonstrated that there was significant differences (P < 0.01) between the fertile (N = 38) and the infertile (N = 16) samples in percentage normal morphology of seminal sperm (67.8 ± 1.8% vs 56.2 ± 2.6%) and curvilinear velocity of swim-up sperm (89.2 ± 3.5 vs 68.2 ± 7.2 μ/sec.). The fertilization rates correlated with the percentage normal morphology of seminal sperm ( r = 0.3868; P < 0.005) and velocity of swim-up sperm (r = 0.3842; P < 0.005). Multivariate stepwise discriminant analysis demonstrated that these two sperm parameters in combination were capable of predicting the fertilization rate with 74.1% accuracy. Our results indicate that seminal sperm morphology, coupled with computer-assisted image analysis of movement characteristics of swim-up sperm, can help to predict the outcome of in vitro fertilization of human oocytes.en_US
dc.languageengen_US
dc.relation.ispartofJournal of In Vitro Fertilization and Embryo Transferen_US
dc.subject.meshFemaleen_US
dc.subject.meshFertilization In Vitroen_US
dc.subject.meshHumansen_US
dc.subject.meshMaleen_US
dc.subject.meshOocytes - Physiologyen_US
dc.subject.meshPredictive Value Of Testsen_US
dc.subject.meshPregnancyen_US
dc.subject.meshSperm Capacitationen_US
dc.subject.meshSpermatozoa - Cytologyen_US
dc.titlePredictive value of sperm morphology and movement characteristics in the outcome of in vitro fertilization of human oocytesen_US
dc.typeArticleen_US
dc.identifier.emailHo, PC:pcho@hku.hken_US
dc.identifier.authorityHo, PC=rp00325en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/BF01130778-
dc.identifier.pmid2677189-
dc.identifier.scopuseid_2-s2.0-0024417464en_US
dc.identifier.volume6en_US
dc.identifier.issue3en_US
dc.identifier.spage142en_US
dc.identifier.epage148en_US
dc.identifier.isiWOS:A1989AP70200004-
dc.identifier.scopusauthoridChan, SYW=7404255960en_US
dc.identifier.scopusauthoridWang, C=7501631357en_US
dc.identifier.scopusauthoridChan, STH=24368283200en_US
dc.identifier.scopusauthoridHo, PC=7402211440en_US
dc.identifier.scopusauthoridSo, WWK=7004974015en_US
dc.identifier.scopusauthoridChan, YF=7403676061en_US
dc.identifier.scopusauthoridMa, HK=7403095603en_US

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