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Article: A new method for the simultaneous determination of androstenedione, testosterone, 11-oxotestosterone and 11β-OH-testosterone in fish plasma using combined techniques of celite chromatography and radioimmunoassay

TitleA new method for the simultaneous determination of androstenedione, testosterone, 11-oxotestosterone and 11β-OH-testosterone in fish plasma using combined techniques of celite chromatography and radioimmunoassay
Authors
Issue Date1986
Citation
Journal Of Steroid Biochemistry, 1986, v. 25 n. 6, p. 1013-1021 How to Cite?
AbstractA new method for the simultaneous determination of androstenedione, testosterone, 11-oxotestosterone and 11β-hydroxy-testosterone in teleost plasma has been developed. Steroids extracted from the plasma were first separated by Celite chromatography using ethanediol as the stationary phase and different concentrations of ethyl acetate in iso-octane as the eluting solvents. Androstenedione, testosterone, 11-oxotestosterone and 11β-OH-testosterone were eluted successively with 0, 10, 15 and 40% of ethyl acetate in iso-octane. The eluted steroid fractions were then quantitatively determined by radioimmunoassay. Data on the accuracy, precision, and sensitivity were presented showing that this new assay system was precise and reproducible. As an illustration of its application, this method was used in the present study to determine the plasma androgen levels in Monopterus albus and in methyl-testosterone treated Tilapia mossambicus.
Persistent Identifierhttp://hdl.handle.net/10722/173113
ISSN
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChan, STHen_US
dc.contributor.authorYeung, WSBen_US
dc.date.accessioned2012-10-30T06:28:00Z-
dc.date.available2012-10-30T06:28:00Z-
dc.date.issued1986en_US
dc.identifier.citationJournal Of Steroid Biochemistry, 1986, v. 25 n. 6, p. 1013-1021en_US
dc.identifier.issn0022-4731en_US
dc.identifier.urihttp://hdl.handle.net/10722/173113-
dc.description.abstractA new method for the simultaneous determination of androstenedione, testosterone, 11-oxotestosterone and 11β-hydroxy-testosterone in teleost plasma has been developed. Steroids extracted from the plasma were first separated by Celite chromatography using ethanediol as the stationary phase and different concentrations of ethyl acetate in iso-octane as the eluting solvents. Androstenedione, testosterone, 11-oxotestosterone and 11β-OH-testosterone were eluted successively with 0, 10, 15 and 40% of ethyl acetate in iso-octane. The eluted steroid fractions were then quantitatively determined by radioimmunoassay. Data on the accuracy, precision, and sensitivity were presented showing that this new assay system was precise and reproducible. As an illustration of its application, this method was used in the present study to determine the plasma androgen levels in Monopterus albus and in methyl-testosterone treated Tilapia mossambicus.en_US
dc.languageengen_US
dc.relation.ispartofJournal of Steroid Biochemistryen_US
dc.subject.meshAndrostenedione - Blooden_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibody Specificityen_US
dc.subject.meshChromatographyen_US
dc.subject.meshDiatomaceous Earthen_US
dc.subject.meshFishes - Blooden_US
dc.subject.meshHydroxytestosterones - Blooden_US
dc.subject.meshImmune Sera - Immunologyen_US
dc.subject.meshRadioimmunoassayen_US
dc.subject.meshTestosterone - Analogs & Derivatives - Blooden_US
dc.titleA new method for the simultaneous determination of androstenedione, testosterone, 11-oxotestosterone and 11β-OH-testosterone in fish plasma using combined techniques of celite chromatography and radioimmunoassayen_US
dc.typeArticleen_US
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_US
dc.identifier.authorityYeung, WSB=rp00331en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0022-4731(86)90338-9-
dc.identifier.pmid3795949-
dc.identifier.scopuseid_2-s2.0-0023018914en_US
dc.identifier.volume25en_US
dc.identifier.issue6en_US
dc.identifier.spage1013en_US
dc.identifier.epage1021en_US
dc.identifier.isiWOS:A1986F432400017-
dc.identifier.scopusauthoridChan, STH=24368283200en_US
dc.identifier.scopusauthoridYeung, WSB=7102370745en_US

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