File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Hypermethylation of the p16 gene in nasopharyngeal carcinoma

TitleHypermethylation of the p16 gene in nasopharyngeal carcinoma
Authors
Issue Date1996
PublisherAmerican Association for Cancer Research. The Journal's web site is located at http://cancerres.aacrjournals.org/
Citation
Cancer Research, 1996, v. 56 n. 12, p. 2721-2725 How to Cite?
AbstractWe have recently reported that inactivation of the p16 gene by mutation and deletion is common in nasopharyngeal carcinoma (NPC). The present study demonstrates that hypermethylation of the 5' CpG island can serve as an alternative mechanism for inactivation of the p16 gene in this tumor. Using Southern blotting analysis and multiplex PCR, aberrant methylation of the 5' CpG island of the p16 gene was found in a NPC xenograft (xeno-666) and 6 (22%) of 27 primary tumors, but not in normal tissues of the nasopharynx. In the NPC xenograft (xeno-666) and its newly derived cell line (cell-666), both showing hypermethylation of the p16 gene, no p16 gene expression was found. After treatment with 5-aza-2'-deoxycytidine, reexpression of the p16 gene was detected in the cell line cell-666. These findings suggest that aberrant methylation of the 5' CpG island may participate in the transcriptional inactivation of the p16 gene in NPC. The present results further support that the p16 gene is the critical target on chromosome 9p21 for inactivation during the development of this disease.
Persistent Identifierhttp://hdl.handle.net/10722/173047
ISSN
2015 Impact Factor: 8.556
2015 SCImago Journal Rankings: 5.372
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLo, KWen_US
dc.contributor.authorCheung, STen_US
dc.contributor.authorLeung, SFen_US
dc.contributor.authorVan Hasselt, Aen_US
dc.contributor.authorTsang, YSen_US
dc.contributor.authorMak, KFen_US
dc.contributor.authorChung, YFen_US
dc.contributor.authorWoo, JKSen_US
dc.contributor.authorLee, JCKen_US
dc.contributor.authorHuang, DPen_US
dc.date.accessioned2012-10-30T06:26:58Z-
dc.date.available2012-10-30T06:26:58Z-
dc.date.issued1996en_US
dc.identifier.citationCancer Research, 1996, v. 56 n. 12, p. 2721-2725en_US
dc.identifier.issn0008-5472en_US
dc.identifier.urihttp://hdl.handle.net/10722/173047-
dc.description.abstractWe have recently reported that inactivation of the p16 gene by mutation and deletion is common in nasopharyngeal carcinoma (NPC). The present study demonstrates that hypermethylation of the 5' CpG island can serve as an alternative mechanism for inactivation of the p16 gene in this tumor. Using Southern blotting analysis and multiplex PCR, aberrant methylation of the 5' CpG island of the p16 gene was found in a NPC xenograft (xeno-666) and 6 (22%) of 27 primary tumors, but not in normal tissues of the nasopharynx. In the NPC xenograft (xeno-666) and its newly derived cell line (cell-666), both showing hypermethylation of the p16 gene, no p16 gene expression was found. After treatment with 5-aza-2'-deoxycytidine, reexpression of the p16 gene was detected in the cell line cell-666. These findings suggest that aberrant methylation of the 5' CpG island may participate in the transcriptional inactivation of the p16 gene in NPC. The present results further support that the p16 gene is the critical target on chromosome 9p21 for inactivation during the development of this disease.en_US
dc.languageengen_US
dc.publisherAmerican Association for Cancer Research. The Journal's web site is located at http://cancerres.aacrjournals.org/en_US
dc.relation.ispartofCancer Researchen_US
dc.subject.meshCarrier Proteins - Geneticsen_US
dc.subject.meshCyclin-Dependent Kinase Inhibitor P16en_US
dc.subject.meshGene Expression Regulation, Neoplasticen_US
dc.subject.meshGenes, Tumor Suppressor - Geneticsen_US
dc.subject.meshHela Cells - Metabolismen_US
dc.subject.meshHumansen_US
dc.subject.meshMethylationen_US
dc.subject.meshNasopharyngeal Neoplasms - Metabolismen_US
dc.subject.meshPolymerase Chain Reactionen_US
dc.subject.meshTransplantation, Heterologousen_US
dc.subject.meshTumor Cells, Cultureden_US
dc.titleHypermethylation of the p16 gene in nasopharyngeal carcinomaen_US
dc.typeArticleen_US
dc.identifier.emailCheung, ST: stcheung@hkucc.hku.hken_US
dc.identifier.authorityCheung, ST=rp00457en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid8665502-
dc.identifier.scopuseid_2-s2.0-9344271110en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-9344271110&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume56en_US
dc.identifier.issue12en_US
dc.identifier.spage2721en_US
dc.identifier.epage2725en_US
dc.identifier.isiWOS:A1996UP66500011-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLo, KW=7402101603en_US
dc.identifier.scopusauthoridCheung, ST=7202473497en_US
dc.identifier.scopusauthoridLeung, SF=7202044876en_US
dc.identifier.scopusauthoridVan Hasselt, A=6603932076en_US
dc.identifier.scopusauthoridTsang, YS=7007101172en_US
dc.identifier.scopusauthoridMak, KF=8096354000en_US
dc.identifier.scopusauthoridChung, YF=7404387227en_US
dc.identifier.scopusauthoridWoo, JKS=27268094700en_US
dc.identifier.scopusauthoridLee, JCK=7601456915en_US
dc.identifier.scopusauthoridHuang, DP=7403891486en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats