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Article: Identification of pyruvate kinase type M2 as potential oncoprotein in squamous cell carcinoma of tongue through microRNA profiling

TitleIdentification of pyruvate kinase type M2 as potential oncoprotein in squamous cell carcinoma of tongue through microRNA profiling
Authors
Issue Date2008
PublisherJohn Wiley & Sons, Inc.. The Journal's web site is located at http://www3.interscience.wiley.com/journal/29331/home
Citation
International Journal Of Cancer, 2008, v. 123 n. 2, p. 251-257 How to Cite?
AbstractMicroRNAs (miRNAs) are noncoding RNAs with specific regulatory role in gene expression. Recent reports suggested their involvement in human malignancies. Currently, there is no information concerning miRNA expression and functions in squamous cell carcinoma (SCC) of tongue. In this study, we evaluated the expression patterns of 156 mature miRNAs in tongue SCC using Taqman-based microRNA assays. Of these 156 miRNAs, miR-133a and miR-133b were significantly reduced in tongue SCC cells in comparison with the paired normal epithelial cells. Tongue SCC cell lines transfected with miR-133a and miR-133b precursors displayed reduction in proliferation rate. In addition, the number of apoptotic cells was increased in response to the introduction of precursors. Computational target gene prediction suggested that both miR-133a and miR-133b are targeting transcript of pyruvate kinase type M2 (PKM2), a potential oncogene in solid cancers. In tongue SCC cell lines, PKM2 expression was reduced in response to miR-133a and miR-133b precursors transfection. Immunohistochemical staining results of tongue SCC tissues suggested that PKM2 was overexpressed in tongue SCC and was associated with the downregulation of miR-133a and miR-133b. Our results suggested that aberrant reduction of miR-133a and miR-133b was associated with the dysregulation of PKM2 in SCC of tongue. © 2008 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/172971
ISSN
2015 Impact Factor: 5.531
2015 SCImago Journal Rankings: 2.657
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWong, TSen_US
dc.contributor.authorLiu, XBen_US
dc.contributor.authorHo, ACWen_US
dc.contributor.authorYuen, APWen_US
dc.contributor.authorNg, RWMen_US
dc.contributor.authorWei, WIen_US
dc.date.accessioned2012-10-30T06:26:08Z-
dc.date.available2012-10-30T06:26:08Z-
dc.date.issued2008en_US
dc.identifier.citationInternational Journal Of Cancer, 2008, v. 123 n. 2, p. 251-257en_US
dc.identifier.issn0020-7136en_US
dc.identifier.urihttp://hdl.handle.net/10722/172971-
dc.description.abstractMicroRNAs (miRNAs) are noncoding RNAs with specific regulatory role in gene expression. Recent reports suggested their involvement in human malignancies. Currently, there is no information concerning miRNA expression and functions in squamous cell carcinoma (SCC) of tongue. In this study, we evaluated the expression patterns of 156 mature miRNAs in tongue SCC using Taqman-based microRNA assays. Of these 156 miRNAs, miR-133a and miR-133b were significantly reduced in tongue SCC cells in comparison with the paired normal epithelial cells. Tongue SCC cell lines transfected with miR-133a and miR-133b precursors displayed reduction in proliferation rate. In addition, the number of apoptotic cells was increased in response to the introduction of precursors. Computational target gene prediction suggested that both miR-133a and miR-133b are targeting transcript of pyruvate kinase type M2 (PKM2), a potential oncogene in solid cancers. In tongue SCC cell lines, PKM2 expression was reduced in response to miR-133a and miR-133b precursors transfection. Immunohistochemical staining results of tongue SCC tissues suggested that PKM2 was overexpressed in tongue SCC and was associated with the downregulation of miR-133a and miR-133b. Our results suggested that aberrant reduction of miR-133a and miR-133b was associated with the dysregulation of PKM2 in SCC of tongue. © 2008 Wiley-Liss, Inc.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc.. The Journal's web site is located at http://www3.interscience.wiley.com/journal/29331/homeen_US
dc.relation.ispartofInternational Journal of Canceren_US
dc.rightsInternational Journal of Cancer. Copyright © John Wiley & Sons, Inc.-
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshCarcinoma, Squamous Cell - Chemistry - Pathologyen_US
dc.subject.meshCell Line, Tumoren_US
dc.subject.meshDown-Regulationen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_US
dc.subject.meshFemaleen_US
dc.subject.meshGene Expression Profilingen_US
dc.subject.meshGene Expression Regulation, Neoplasticen_US
dc.subject.meshHumansen_US
dc.subject.meshMaleen_US
dc.subject.meshMicrornasen_US
dc.subject.meshMicrodissectionen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshOncogene Proteins - Analysisen_US
dc.subject.meshPyruvate Kinase - Analysisen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.subject.meshTongue Neoplasms - Chemistry - Pathologyen_US
dc.subject.meshUp-Regulationen_US
dc.titleIdentification of pyruvate kinase type M2 as potential oncoprotein in squamous cell carcinoma of tongue through microRNA profilingen_US
dc.typeArticleen_US
dc.identifier.emailWong, TS: thiansze@graduate.hku.hken_US
dc.identifier.emailWei, WI: hrmswwi@hku.hken_US
dc.identifier.authorityWong, TS=rp00478en_US
dc.identifier.authorityWei, WI=rp00323en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/ijc.23583en_US
dc.identifier.pmid18464261-
dc.identifier.scopuseid_2-s2.0-44949142045en_US
dc.identifier.hkuros154665-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-44949142045&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume123en_US
dc.identifier.issue2en_US
dc.identifier.spage251en_US
dc.identifier.epage257en_US
dc.identifier.eissn1097-0215-
dc.identifier.isiWOS:000256760300002-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridWong, TS=7403531328en_US
dc.identifier.scopusauthoridLiu, XB=26642902000en_US
dc.identifier.scopusauthoridHo, ACW=16202695800en_US
dc.identifier.scopusauthoridYuen, APW=7006290111en_US
dc.identifier.scopusauthoridNg, RWM=7102153861en_US
dc.identifier.scopusauthoridWei, WI=7403321552en_US

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