Gastric cancer associated glycoprotein with bisection GlcNAc structure

Abstract

In this report, the clinical significance of gastrointestinal cancer associated glycoprotein with bisection GlcNAc structure was investigated by affinity assay and immunobiochemical methods. The results of 51 Cr-release assay suggested that this glycoprotein could inhibit the cytotoxicity of NK and LAK cells for their target cells. The results of enzyme-linked immunoassay suggested that the expression of gastric cancer associated glycoproteins with bisection GlcNAc oligosaccharides structure was higher in serum of the patient with gastrointestinal cancer than that in serum of the patient with benign disease. (p<0.001). The sensitivity of this analysis procedure were 59.9% (191/320) for diagnosis of gastric cancer and 64.1% (100/156) for diagnosis of colorectal cancer. Its speciality was 91.8% for diagnosis of tumor. The results also indicated that the level of gastric cancer oligosaccharides in serum related to the differentiation type of cancer and the immunological activity of LAK cells. The present study provided a new approach for research on tumor marker and glycosylation associated with gastro-intestinal cancer.