File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: In vivo MR imaging of nanometer magnetically labeled bone marrow stromal cells transplanted via portal vein in rat liver

TitleIn vivo MR imaging of nanometer magnetically labeled bone marrow stromal cells transplanted via portal vein in rat liver
超順磁性氧化鐵標記大鼠骨髓基質細胞經門靜脈移植MRI活體示蹤的研究
Authors
KeywordsAnimals, Laboratory
Cell Transplantation
Magnetic Resonance Imaging
Portal Vein
Stem Cells
Issue Date2006
PublisherZhonghua Yixuehui Zazhishe. The Journal's web site is located at http://med.wanfangdata.com.cn/Journal/zhfsx.aspx
Citation
Chinese Journal Of Radiology, 2006, v. 40 n. 2, p. 133-138 How to Cite?
中華放射學雜誌, 2006, v. 40 n. 2, p. 133-138 How to Cite?
AbstractObjective: To evaluate in vivo magnetic resonance imaging with a conventional 1.5-T system for tracking of intra-portal vein transplantation nanometer magnetically labeled BMSCs in rat liver. Methods: BMSCs were isolated from 5 SD rats bone marrow with the density gradient centrifugation method. Then BMSCs were labeled with nanometer superpara-magnetic iron oxide and transfection agent. Cell labeling efficiency was assessed with determination of the percentage of Perls Prussian blue stain. Then BMSCs transplanted into normal rats' livers via portal vein. The receipts were divided into 5 groups, including sham control,2 h,3 d,7 d and 2 w after transplantation. Follow-up serial T1 WI, T2 WI and T2*-weighted gradient-echo MR imaging were performed at 1.5 T MRI system. MR imaging findings were compared with histology. Results: Cell labeling efficiency was more than 95% by Perls Prussian blue stain. After transplantation of labeled BMSCs via portal vein, livers had diffuse granular signal intensity appearance in T2* WI MRI. Cells were detected for up to 2 w in receipts' livers. At histologic analysis, signal intensity loss correlated with iron-loaded cells. Conclusion: MR imaging could aid in monitoring of magnetically labeled BMSCs administered via portal vein in vivo.
Persistent Identifierhttp://hdl.handle.net/10722/172908
ISSN
2015 SCImago Journal Rankings: 0.101
References

 

DC FieldValueLanguage
dc.contributor.authorWang, Pen_US
dc.contributor.authorWang, JHen_US
dc.contributor.authorYan, ZPen_US
dc.contributor.authorHu, MYen_US
dc.contributor.authorXu, PJen_US
dc.contributor.authorZhou, MLen_US
dc.contributor.authorYan, FHen_US
dc.contributor.authorFan, STen_US
dc.contributor.authorLuk, JMen_US
dc.date.accessioned2012-10-30T06:25:44Z-
dc.date.available2012-10-30T06:25:44Z-
dc.date.issued2006en_US
dc.identifier.citationChinese Journal Of Radiology, 2006, v. 40 n. 2, p. 133-138en_US
dc.identifier.citation中華放射學雜誌, 2006, v. 40 n. 2, p. 133-138-
dc.identifier.issn1005-1201en_US
dc.identifier.urihttp://hdl.handle.net/10722/172908-
dc.description.abstractObjective: To evaluate in vivo magnetic resonance imaging with a conventional 1.5-T system for tracking of intra-portal vein transplantation nanometer magnetically labeled BMSCs in rat liver. Methods: BMSCs were isolated from 5 SD rats bone marrow with the density gradient centrifugation method. Then BMSCs were labeled with nanometer superpara-magnetic iron oxide and transfection agent. Cell labeling efficiency was assessed with determination of the percentage of Perls Prussian blue stain. Then BMSCs transplanted into normal rats' livers via portal vein. The receipts were divided into 5 groups, including sham control,2 h,3 d,7 d and 2 w after transplantation. Follow-up serial T1 WI, T2 WI and T2*-weighted gradient-echo MR imaging were performed at 1.5 T MRI system. MR imaging findings were compared with histology. Results: Cell labeling efficiency was more than 95% by Perls Prussian blue stain. After transplantation of labeled BMSCs via portal vein, livers had diffuse granular signal intensity appearance in T2* WI MRI. Cells were detected for up to 2 w in receipts' livers. At histologic analysis, signal intensity loss correlated with iron-loaded cells. Conclusion: MR imaging could aid in monitoring of magnetically labeled BMSCs administered via portal vein in vivo.en_US
dc.languagechien_US
dc.publisherZhonghua Yixuehui Zazhishe. The Journal's web site is located at http://med.wanfangdata.com.cn/Journal/zhfsx.aspx-
dc.relation.ispartofChinese Journal of Radiologyen_US
dc.relation.ispartof中華放射學雜誌-
dc.subjectAnimals, Laboratoryen_US
dc.subjectCell Transplantationen_US
dc.subjectMagnetic Resonance Imagingen_US
dc.subjectPortal Veinen_US
dc.subjectStem Cellsen_US
dc.titleIn vivo MR imaging of nanometer magnetically labeled bone marrow stromal cells transplanted via portal vein in rat liveren_US
dc.title超順磁性氧化鐵標記大鼠骨髓基質細胞經門靜脈移植MRI活體示蹤的研究-
dc.typeArticleen_US
dc.identifier.emailFan, ST: stfan@hku.hken_US
dc.identifier.emailLuk, JM: jmluk@hkucc.hku.hken_US
dc.identifier.authorityFan, ST=rp00355en_US
dc.identifier.authorityLuk, JM=rp00349en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.3760/j.issn:1005-1201.2006.02.005-
dc.identifier.scopuseid_2-s2.0-33645546579en_US
dc.identifier.hkuros137565-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33645546579&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume40en_US
dc.identifier.issue2en_US
dc.identifier.spage133en_US
dc.identifier.epage138en_US
dc.publisher.placeChinaen_US
dc.identifier.scopusauthoridWang, P=7405460611en_US
dc.identifier.scopusauthoridWang, JH=8898010700en_US
dc.identifier.scopusauthoridYan, ZP=7402519656en_US
dc.identifier.scopusauthoridHu, MY=7402639252en_US
dc.identifier.scopusauthoridXu, PJ=7202215783en_US
dc.identifier.scopusauthoridZhou, ML=7403506132en_US
dc.identifier.scopusauthoridYan, FH=12789764400en_US
dc.identifier.scopusauthoridFan, ST=7402678224en_US
dc.identifier.scopusauthoridLuk, JM=7006777791en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats