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Article: Porcine liver: Morphologic characteristics and cell viability at experimental radiofrequency ablation with internally cooled electrodes

TitlePorcine liver: Morphologic characteristics and cell viability at experimental radiofrequency ablation with internally cooled electrodes
Authors
Issue Date2005
PublisherRadiological Society of North America, Inc. The Journal's web site is located at http://radiology.rsnajnls.org
Citation
Radiology, 2005, v. 235 n. 2, p. 478-486 How to Cite?
AbstractPURPOSE: To evaluate morphologic characteristics and cell viability of radiofrequency ablation zones in porcine liver. MATERIALS AND METHODS: Approval of the study protocol was obtained from the Ethics Committee on Use of Live Animals for Teaching and Research at University of Hong Kong. Internally cooled electrodes were used to produce 120 ablated zones ex vivo and 60 ablated zones in vivo with single electrodes (1-, 2-, and 3-cm exposed lengths) or clustered electrodes (1.0-, 2.0-, and 2.5-cm exposed lengths) at 4, 8, 12, and 16 minutes of ablation (ex vivo) and 8 and 12 minutes of ablation (in vivo). Morphologic measurements of each ablated zone were performed. Cell viability in each ablated zone was assessed qualitatively with histochemical staining and quantitatively with measurement of intracellular adenosine 5′-triphosphate (ATP) concentration. RESULTS: Exposed length of electrode (coefficient = 0.79, standard error = 0.04, P < .001), duration of ablation (coefficient = 0.14, standard error = 0.01, P < .001), and clustered electrode design (coefficient = 1.21, standard error = 0.05, P < .001) were independent factors that affected minimal transverse diameter and volume of ablated zone in ex vivo study. Similar morphologic characteristics existed among ablated zones in in vivo study. Mean distance of ablation beyond the electrode tip remained constant (ex vivo, 1.0 cm ± 0.08 [standard deviation]; in vivo, 0.5 cm ± 0.05) regardless of different ablation conditions. Histochemical staining revealed no viable hepatocytes from center to margins of white zone in each ablated area. Mean intracellular ATP concentration in margins of white zone (9.5 × 10-12 mol/μg DNA ± 1.43) was lower than that in red zone (4088 × 10-12 mol/μg DNA ± 65.97, P < .001) and in adjacent normal liver (4528 × 10-12 mol/μg DNA ± 52.74, P < .001). CONCLUSION: Distance of ablation beyond the tip of the electrode remained constant (ex vivo, 1.0 cm; in vivo, 0.5 cm) with different conditions of ablation. Complete and uniform cellular destruction was achieved in the white zone of ablated area. © RSNA, 2005.
Persistent Identifierhttp://hdl.handle.net/10722/172874
ISSN
2015 Impact Factor: 6.798
2015 SCImago Journal Rankings: 3.414
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorNg, KKen_US
dc.contributor.authorLam, CMen_US
dc.contributor.authorPoon, RTen_US
dc.contributor.authorShek, TWen_US
dc.contributor.authorYu, WCen_US
dc.contributor.authorTo, JYTen_US
dc.contributor.authorWo, YHen_US
dc.contributor.authorLau, CPen_US
dc.contributor.authorTang, TCen_US
dc.contributor.authorHo, DWen_US
dc.contributor.authorFan, STen_US
dc.date.accessioned2012-10-30T06:25:28Z-
dc.date.available2012-10-30T06:25:28Z-
dc.date.issued2005en_US
dc.identifier.citationRadiology, 2005, v. 235 n. 2, p. 478-486en_US
dc.identifier.issn0033-8419en_US
dc.identifier.urihttp://hdl.handle.net/10722/172874-
dc.description.abstractPURPOSE: To evaluate morphologic characteristics and cell viability of radiofrequency ablation zones in porcine liver. MATERIALS AND METHODS: Approval of the study protocol was obtained from the Ethics Committee on Use of Live Animals for Teaching and Research at University of Hong Kong. Internally cooled electrodes were used to produce 120 ablated zones ex vivo and 60 ablated zones in vivo with single electrodes (1-, 2-, and 3-cm exposed lengths) or clustered electrodes (1.0-, 2.0-, and 2.5-cm exposed lengths) at 4, 8, 12, and 16 minutes of ablation (ex vivo) and 8 and 12 minutes of ablation (in vivo). Morphologic measurements of each ablated zone were performed. Cell viability in each ablated zone was assessed qualitatively with histochemical staining and quantitatively with measurement of intracellular adenosine 5′-triphosphate (ATP) concentration. RESULTS: Exposed length of electrode (coefficient = 0.79, standard error = 0.04, P < .001), duration of ablation (coefficient = 0.14, standard error = 0.01, P < .001), and clustered electrode design (coefficient = 1.21, standard error = 0.05, P < .001) were independent factors that affected minimal transverse diameter and volume of ablated zone in ex vivo study. Similar morphologic characteristics existed among ablated zones in in vivo study. Mean distance of ablation beyond the electrode tip remained constant (ex vivo, 1.0 cm ± 0.08 [standard deviation]; in vivo, 0.5 cm ± 0.05) regardless of different ablation conditions. Histochemical staining revealed no viable hepatocytes from center to margins of white zone in each ablated area. Mean intracellular ATP concentration in margins of white zone (9.5 × 10-12 mol/μg DNA ± 1.43) was lower than that in red zone (4088 × 10-12 mol/μg DNA ± 65.97, P < .001) and in adjacent normal liver (4528 × 10-12 mol/μg DNA ± 52.74, P < .001). CONCLUSION: Distance of ablation beyond the tip of the electrode remained constant (ex vivo, 1.0 cm; in vivo, 0.5 cm) with different conditions of ablation. Complete and uniform cellular destruction was achieved in the white zone of ablated area. © RSNA, 2005.en_US
dc.languageengen_US
dc.publisherRadiological Society of North America, Inc. The Journal's web site is located at http://radiology.rsnajnls.orgen_US
dc.relation.ispartofRadiologyen_US
dc.subject.meshAdenosine Triphosphate - Analysisen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCatheter Ablation - Instrumentation - Methodsen_US
dc.subject.meshCell Survival - Physiologyen_US
dc.subject.meshElectrodesen_US
dc.subject.meshEquipment Designen_US
dc.subject.meshLiver - Pathology - Surgeryen_US
dc.subject.meshOrgan Culture Techniquesen_US
dc.subject.meshSwineen_US
dc.subject.meshTemperatureen_US
dc.subject.meshTissue Culture Techniquesen_US
dc.subject.meshTreatment Outcomeen_US
dc.titlePorcine liver: Morphologic characteristics and cell viability at experimental radiofrequency ablation with internally cooled electrodesen_US
dc.typeArticleen_US
dc.identifier.emailPoon, RT: poontp@hkucc.hku.hken_US
dc.identifier.authorityPoon, RT=rp00446en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1148/radiol.2352040425en_US
dc.identifier.pmid15798156-
dc.identifier.scopuseid_2-s2.0-20244370194en_US
dc.identifier.hkuros97954-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-20244370194&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume235en_US
dc.identifier.issue2en_US
dc.identifier.spage478en_US
dc.identifier.epage486en_US
dc.identifier.isiWOS:000228571200018-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridNg, KK=7403179075en_US
dc.identifier.scopusauthoridChi, ML=8343493900en_US
dc.identifier.scopusauthoridPoon, RT=7103097223en_US
dc.identifier.scopusauthoridShek, TW=7005479861en_US
dc.identifier.scopusauthoridWan, CY=8343494200en_US
dc.identifier.scopusauthoridTo, JY=6603618643en_US
dc.identifier.scopusauthoridYim, HW=8343494400en_US
dc.identifier.scopusauthoridLau, CP=8086563300en_US
dc.identifier.scopusauthoridTang, TC=36867095200en_US
dc.identifier.scopusauthoridHo, DW=7402971906en_US
dc.identifier.scopusauthoridSheung, TF=6506234707en_US

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