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Article: Presence of filterable and nonfilterable mRNA in the plasma of cancer patients and healthy individuals

TitlePresence of filterable and nonfilterable mRNA in the plasma of cancer patients and healthy individuals
Authors
Issue Date2002
PublisherAmerican Association for Clinical Chemistry, Inc. The Journal's web site is located at http://www.clinchem.org
Citation
Clinical Chemistry, 2002, v. 48 n. 8, p. 1212-1217 How to Cite?
AbstractBackground: As RNA is labile, we investigated whether circulating RNA in human plasma may be present in a particle-associated form. Methods: Blood was collected from 27 healthy individuals and 16 hepatocellular carcinoma (HCC) patients. The plasma from each individual was processed by two means: filtration through filters with different pore sizes (from 5 μm to 0.22 μm) and ultracentrifugation. We assessed plasma RNA content by a real-time quantitative reverse transcription-PCR assay for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) transcripts and plasma DNA by a real-time quantitative PCR assay for the β-globin gene. Results: The plasma GAPDH mRNA concentrations in the healthy individuals were significantly different in every pair of these filter sizes (P <0.05 for each pair). Overall, the plasma GAPDH mRNA concentration was higher by a median of 15-fold (interquartile range, 10- to 24-fold) in the paired unfiltered sample than in the sample filtered through a 0.22 μm filter. In contrast, no significant difference was seen in β-globin DNA concentrations among different pore-size-filtered plasma samples (P = 0.455). Similarly, a significant difference was observed for RNA, but not DNA, between unfiltered plasma and ultracentrifuged plasma (P <0.05). No significant difference in GAPDH mRNA concentrations was seen between the 0.22-μm-filtered plasma samples and the ultracentrifuged plasma samples (P >0.05). In HCC patients, filtration with a 0.22 μm filter produced a median 9.3-fold (interquartile range, 6.9- to 311-fold) reduction in GAPDH mRNA concentration in plasma. Plasma GAPDH mRNA concentrations in HCC patients were significantly higher than those in healthy individuals, both with or without filtration (P <0.05 for filtered plasma samples; P <0.005 for unfiltered plasma sampies). Conclusions: A substantial proportion of plasma mRNA species is particle-associated. In HCC patients, both circulating particle- and non-particle-associated plasma RNA are increased. © 2002 American Association for Clinical Chemistry.
Persistent Identifierhttp://hdl.handle.net/10722/172872
ISSN
2015 Impact Factor: 7.457
2015 SCImago Journal Rankings: 2.472
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorNg, EKOen_US
dc.contributor.authorTsui, NBYen_US
dc.contributor.authorLam, NYLen_US
dc.contributor.authorChiu, RWKen_US
dc.contributor.authorYu, SCHen_US
dc.contributor.authorWong, SCCen_US
dc.contributor.authorLo, ESFen_US
dc.contributor.authorRainer, THen_US
dc.contributor.authorJohnson, PJen_US
dc.contributor.authorLo, YMDen_US
dc.date.accessioned2012-10-30T06:25:27Z-
dc.date.available2012-10-30T06:25:27Z-
dc.date.issued2002en_US
dc.identifier.citationClinical Chemistry, 2002, v. 48 n. 8, p. 1212-1217en_US
dc.identifier.issn0009-9147en_US
dc.identifier.urihttp://hdl.handle.net/10722/172872-
dc.description.abstractBackground: As RNA is labile, we investigated whether circulating RNA in human plasma may be present in a particle-associated form. Methods: Blood was collected from 27 healthy individuals and 16 hepatocellular carcinoma (HCC) patients. The plasma from each individual was processed by two means: filtration through filters with different pore sizes (from 5 μm to 0.22 μm) and ultracentrifugation. We assessed plasma RNA content by a real-time quantitative reverse transcription-PCR assay for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) transcripts and plasma DNA by a real-time quantitative PCR assay for the β-globin gene. Results: The plasma GAPDH mRNA concentrations in the healthy individuals were significantly different in every pair of these filter sizes (P <0.05 for each pair). Overall, the plasma GAPDH mRNA concentration was higher by a median of 15-fold (interquartile range, 10- to 24-fold) in the paired unfiltered sample than in the sample filtered through a 0.22 μm filter. In contrast, no significant difference was seen in β-globin DNA concentrations among different pore-size-filtered plasma samples (P = 0.455). Similarly, a significant difference was observed for RNA, but not DNA, between unfiltered plasma and ultracentrifuged plasma (P <0.05). No significant difference in GAPDH mRNA concentrations was seen between the 0.22-μm-filtered plasma samples and the ultracentrifuged plasma samples (P >0.05). In HCC patients, filtration with a 0.22 μm filter produced a median 9.3-fold (interquartile range, 6.9- to 311-fold) reduction in GAPDH mRNA concentration in plasma. Plasma GAPDH mRNA concentrations in HCC patients were significantly higher than those in healthy individuals, both with or without filtration (P <0.05 for filtered plasma samples; P <0.005 for unfiltered plasma sampies). Conclusions: A substantial proportion of plasma mRNA species is particle-associated. In HCC patients, both circulating particle- and non-particle-associated plasma RNA are increased. © 2002 American Association for Clinical Chemistry.en_US
dc.languageengen_US
dc.publisherAmerican Association for Clinical Chemistry, Inc. The Journal's web site is located at http://www.clinchem.orgen_US
dc.relation.ispartofClinical Chemistryen_US
dc.subject.meshCarcinoma, Hepatocellular - Blood - Diagnosisen_US
dc.subject.meshFiltrationen_US
dc.subject.meshGlyceraldehyde-3-Phosphate Dehydrogenases - Blood - Geneticsen_US
dc.subject.meshHumansen_US
dc.subject.meshLiver Neoplasms - Blood - Diagnosisen_US
dc.subject.meshPlasmaen_US
dc.subject.meshRna, Messenger - Blooden_US
dc.subject.meshReference Valuesen_US
dc.subject.meshReproducibility Of Resultsen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.subject.meshUltracentrifugationen_US
dc.titlePresence of filterable and nonfilterable mRNA in the plasma of cancer patients and healthy individualsen_US
dc.typeArticleen_US
dc.identifier.emailNg, EKO: ngko@hku.hken_US
dc.identifier.authorityNg, EKO=rp01364en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid12142376-
dc.identifier.scopuseid_2-s2.0-18444415477en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-18444415477&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume48en_US
dc.identifier.issue8en_US
dc.identifier.spage1212en_US
dc.identifier.epage1217en_US
dc.identifier.isiWOS:000176996500012-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridNg, EKO=21135553700en_US
dc.identifier.scopusauthoridTsui, NBY=6602401748en_US
dc.identifier.scopusauthoridLam, NYL=7101750743en_US
dc.identifier.scopusauthoridChiu, RWK=7103038413en_US
dc.identifier.scopusauthoridYu, SCH=25960517200en_US
dc.identifier.scopusauthoridWong, SCC=7404590793en_US
dc.identifier.scopusauthoridLo, ESF=7101705996en_US
dc.identifier.scopusauthoridRainer, TH=7004489495en_US
dc.identifier.scopusauthoridJohnson, PJ=7405661637en_US
dc.identifier.scopusauthoridLo, YMD=7401935391en_US

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