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Article: Isolation and chromosomal mapping of human glycogen synthase kinase-3α and -3β encoding genes

TitleIsolation and chromosomal mapping of human glycogen synthase kinase-3α and -3β encoding genes
Authors
Issue Date1998
PublisherN R C Research Press. The Journal's web site is located at http://pubs.nrc-cnrc.gc.ca/cgi-bin/rp/rp2_desc_e?gen
Citation
Genome, 1998, v. 41 n. 5, p. 720-727 How to Cite?
AbstractGlycogen synthase kinase-3 (GSK-3) is a serine-threonine kinase that exists as two isoforms, α and β, encoded by separate genes. Phosphorylation targets include a variety of cytoplasmic and nuclear proteins. Recent studies found that neurofilaments, amyloid precursor protein, and tau proteins are substrates of GSK-3 and that aberrant phosphorylation of these proteins is implicated in pathologies of the nervous system. To analyse the organisation of these two genes, a YAC library was screened by polymerase chain reaction, using primers specific for human GSK-3α and GSK-3β cDNA. TWo clones, 220 and 285 kb m size, containing the complete GSK-3α coding sequence, and two clones, 365 and 285 kb in size, containing the 5' coding sequence of GSK-3β, were isolated. By somatic cell hybrid panel DNA amplification and radiation hybrid mapping, GSK-3α was found to be located at 19q13.2. On the other hand, by somatic cell hybrid panel DNA amplification and fluorescence in situ hybridisation using the 285-kb YAC clone, GSK-3β was mapped to 3q13.3.
Persistent Identifierhttp://hdl.handle.net/10722/172751
ISSN
2015 Impact Factor: 1.356
2015 SCImago Journal Rankings: 0.666
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorShaw, PCen_US
dc.contributor.authorDavies, AFen_US
dc.contributor.authorLau, KFen_US
dc.contributor.authorGarciaBarcelo, Men_US
dc.contributor.authorWaye, MMYen_US
dc.contributor.authorLovestone, Sen_US
dc.contributor.authorMiller, CCJen_US
dc.contributor.authorAnderton, BHen_US
dc.date.accessioned2012-10-30T06:24:42Z-
dc.date.available2012-10-30T06:24:42Z-
dc.date.issued1998en_US
dc.identifier.citationGenome, 1998, v. 41 n. 5, p. 720-727en_US
dc.identifier.issn0831-2796en_US
dc.identifier.urihttp://hdl.handle.net/10722/172751-
dc.description.abstractGlycogen synthase kinase-3 (GSK-3) is a serine-threonine kinase that exists as two isoforms, α and β, encoded by separate genes. Phosphorylation targets include a variety of cytoplasmic and nuclear proteins. Recent studies found that neurofilaments, amyloid precursor protein, and tau proteins are substrates of GSK-3 and that aberrant phosphorylation of these proteins is implicated in pathologies of the nervous system. To analyse the organisation of these two genes, a YAC library was screened by polymerase chain reaction, using primers specific for human GSK-3α and GSK-3β cDNA. TWo clones, 220 and 285 kb m size, containing the complete GSK-3α coding sequence, and two clones, 365 and 285 kb in size, containing the 5' coding sequence of GSK-3β, were isolated. By somatic cell hybrid panel DNA amplification and radiation hybrid mapping, GSK-3α was found to be located at 19q13.2. On the other hand, by somatic cell hybrid panel DNA amplification and fluorescence in situ hybridisation using the 285-kb YAC clone, GSK-3β was mapped to 3q13.3.en_US
dc.languageengen_US
dc.publisherN R C Research Press. The Journal's web site is located at http://pubs.nrc-cnrc.gc.ca/cgi-bin/rp/rp2_desc_e?genen_US
dc.relation.ispartofGenomeen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshCalcium-Calmodulin-Dependent Protein Kinases - Geneticsen_US
dc.subject.meshChromosome Mappingen_US
dc.subject.meshChromosomes, Artificial, Yeast - Geneticsen_US
dc.subject.meshChromosomes, Human, Pair 19 - Geneticsen_US
dc.subject.meshChromosomes, Human, Pair 3 - Geneticsen_US
dc.subject.meshCloning, Molecularen_US
dc.subject.meshCricetinaeen_US
dc.subject.meshDna Primers - Geneticsen_US
dc.subject.meshGlycogen Synthase Kinase 3en_US
dc.subject.meshGlycogen Synthase Kinasesen_US
dc.subject.meshHumansen_US
dc.subject.meshHybrid Cellsen_US
dc.subject.meshIn Situ Hybridization, Fluorescenceen_US
dc.subject.meshIsoenzymes - Geneticsen_US
dc.subject.meshMiceen_US
dc.subject.meshPolymerase Chain Reactionen_US
dc.titleIsolation and chromosomal mapping of human glycogen synthase kinase-3α and -3β encoding genesen_US
dc.typeArticleen_US
dc.identifier.emailGarciaBarcelo, M: mmgarcia@hkucc.hku.hken_US
dc.identifier.authorityGarciaBarcelo, M=rp00445en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1139/gen-41-5-720-
dc.identifier.pmid9809441-
dc.identifier.scopuseid_2-s2.0-0032193702en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032193702&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume41en_US
dc.identifier.issue5en_US
dc.identifier.spage720en_US
dc.identifier.epage727en_US
dc.identifier.isiWOS:000076773400012-
dc.publisher.placeCanadaen_US
dc.identifier.scopusauthoridShaw, PC=35599523600en_US
dc.identifier.scopusauthoridDavies, AF=7403658133en_US
dc.identifier.scopusauthoridLau, KF=7401560031en_US
dc.identifier.scopusauthoridGarciaBarcelo, M=6701767303en_US
dc.identifier.scopusauthoridWaye, MMY=7006687733en_US
dc.identifier.scopusauthoridLovestone, S=7005575001en_US
dc.identifier.scopusauthoridMiller, CCJ=8980202200en_US
dc.identifier.scopusauthoridAnderton, BH=7006709314en_US

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