File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Mycobacterial DNA not detected in liver sections from patients with primary billiary cirrhosis

TitleMycobacterial DNA not detected in liver sections from patients with primary billiary cirrhosis
Authors
Issue Date1998
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jhep
Citation
Journal Of Hepatology, 1998, v. 28 n. 3, p. 433-438 How to Cite?
AbstractBackground/Aims: Recent studies in primary biliary cirrhosis have reported the detection of serum antibodies against Mycobacterium gordonae and of mycobacterial DNA in liver sections. The aim of this study was to investigate whether mycobacterial DNA is present in liver biopsy material in primary biliary cirrhosis. Methods: Archival liver biopsy specimens from 11 patients with primary biliary cirrhosis (10 female, mean age 52 years) and 11 patients with autoimmune hepatitis (10 female, mean age 53 years) were identified. Positive control tissue comprised five archival lymph node specimens from patients with tuberculous lymphadenopathy, three of which had stained positive on ZN staining, and also a fiver biopsy specimen from a patient with tuberculous hepatitis (ZN positive). Fixed sections were deparaffinised and DNA was extracted by mechanical disruption with glass beads. DNA was purified by use of diatoms and lysis in guanidinium thiocyanate in a technique previously validated for archival DNA. Primers were directed to amplify a partial 16S ribosomal RNA gene yielding the species-specific character for mycobacteria, and also to amplify the constitutively-expressed human gene GAPDH. Results: The polymerase chain reaction was shown to be capable of detecting 1 fg of M. gordonae DNA in 'spiked' samples, equivalent to 1-5 bacterial cells. No mycobacterial DNA was detected in fiver biopsy samples from either the primary biliary cirrhosis or autoimmune hepatitis groups. Of the tuberculous control sections, mycobacterial DNA was detected in four of five lymph nodes and the liver biopsy specimen. GAPDH amplification was detected in all tested samples from fiver disease and tuberculous control samples. Conclusion: These data do not support a role for mycobacteria in the aetiology of primary biliary cirrhosis.
Persistent Identifierhttp://hdl.handle.net/10722/172748
ISSN
2015 Impact Factor: 10.59
2015 SCImago Journal Rankings: 4.570
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorO'donohue, Jen_US
dc.contributor.authorFidler, Hen_US
dc.contributor.authorGarciaBarcelo, Men_US
dc.contributor.authorNouriAria, Ken_US
dc.contributor.authorWilliams, Ren_US
dc.contributor.authorMcfadden, Jen_US
dc.date.accessioned2012-10-30T06:24:40Z-
dc.date.available2012-10-30T06:24:40Z-
dc.date.issued1998en_US
dc.identifier.citationJournal Of Hepatology, 1998, v. 28 n. 3, p. 433-438en_US
dc.identifier.issn0168-8278en_US
dc.identifier.urihttp://hdl.handle.net/10722/172748-
dc.description.abstractBackground/Aims: Recent studies in primary biliary cirrhosis have reported the detection of serum antibodies against Mycobacterium gordonae and of mycobacterial DNA in liver sections. The aim of this study was to investigate whether mycobacterial DNA is present in liver biopsy material in primary biliary cirrhosis. Methods: Archival liver biopsy specimens from 11 patients with primary biliary cirrhosis (10 female, mean age 52 years) and 11 patients with autoimmune hepatitis (10 female, mean age 53 years) were identified. Positive control tissue comprised five archival lymph node specimens from patients with tuberculous lymphadenopathy, three of which had stained positive on ZN staining, and also a fiver biopsy specimen from a patient with tuberculous hepatitis (ZN positive). Fixed sections were deparaffinised and DNA was extracted by mechanical disruption with glass beads. DNA was purified by use of diatoms and lysis in guanidinium thiocyanate in a technique previously validated for archival DNA. Primers were directed to amplify a partial 16S ribosomal RNA gene yielding the species-specific character for mycobacteria, and also to amplify the constitutively-expressed human gene GAPDH. Results: The polymerase chain reaction was shown to be capable of detecting 1 fg of M. gordonae DNA in 'spiked' samples, equivalent to 1-5 bacterial cells. No mycobacterial DNA was detected in fiver biopsy samples from either the primary biliary cirrhosis or autoimmune hepatitis groups. Of the tuberculous control sections, mycobacterial DNA was detected in four of five lymph nodes and the liver biopsy specimen. GAPDH amplification was detected in all tested samples from fiver disease and tuberculous control samples. Conclusion: These data do not support a role for mycobacteria in the aetiology of primary biliary cirrhosis.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jhepen_US
dc.relation.ispartofJournal of Hepatologyen_US
dc.subject.meshAdulten_US
dc.subject.meshAutoimmune Diseases - Geneticsen_US
dc.subject.meshDna - Analysisen_US
dc.subject.meshDna, Bacterial - Analysisen_US
dc.subject.meshFemaleen_US
dc.subject.meshGenomeen_US
dc.subject.meshHumansen_US
dc.subject.meshLiver - Chemistry - Microbiologyen_US
dc.subject.meshLiver Cirrhosis, Biliary - Genetics - Microbiologyen_US
dc.subject.meshLiver Diseases - Geneticsen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshMycobacterium - Geneticsen_US
dc.subject.meshReference Valuesen_US
dc.subject.meshSensitivity And Specificityen_US
dc.titleMycobacterial DNA not detected in liver sections from patients with primary billiary cirrhosisen_US
dc.typeArticleen_US
dc.identifier.emailGarciaBarcelo, M: mmgarcia@hkucc.hku.hken_US
dc.identifier.authorityGarciaBarcelo, M=rp00445en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/S0168-8278(98)80317-6en_US
dc.identifier.pmid9551681-
dc.identifier.scopuseid_2-s2.0-0032030878en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032030878&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume28en_US
dc.identifier.issue3en_US
dc.identifier.spage433en_US
dc.identifier.epage438en_US
dc.identifier.isiWOS:000072420600011-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridO'Donohue, J=6701759464en_US
dc.identifier.scopusauthoridFidler, H=14050354400en_US
dc.identifier.scopusauthoridGarciaBarcelo, M=6701767303en_US
dc.identifier.scopusauthoridNouriAria, K=7004055442en_US
dc.identifier.scopusauthoridWilliams, R=7409607221en_US
dc.identifier.scopusauthoridMcFadden, J=16185831500en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats