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Article: Specificity of monoclonal antibodies binding to the polysaccharide antigens (Vi, O9) of Salmonella typhi

TitleSpecificity of monoclonal antibodies binding to the polysaccharide antigens (Vi, O9) of Salmonella typhi
Authors
KeywordsLipopolysaccharide
Monoclonal Antibodies
Salmonella Typhi
Serotyping
Vi Polysaccharide
Issue Date1992
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journal.asp?ref=0378-1097&site=1
Citation
Fems Microbiology Letters, 1992, v. 97 n. 1-2, p. 173-178 How to Cite?
AbstractA panel of monoclonal antibodies were generated against the surface polysaccharide antigens of the cell envelope of Salmonella typhi. Four clones (IgM) were specific for the capsular Vi polysaccharide, and one clone (IgG3) reacted selectively with the S. typhi lipopolysaccharide in enzyme immunoassay. On the basis of their reactivity pattern and binding affinity, MATy-V7 (IgM) and MATy-O9 (IgG3) antibodies were selected for further characterization of their antigenic specificity. In an inhibition enzyme immunoassay with rabbit factor-specific anti-Salmonella antibodies as the competing agents, the reactivity of MATy-V7 and MATy-O9 were significantly inhibited by the anti-Vi and anti-O9 antisera, respectively. Moreover, both the Vi- and O9-specific monoclonal antibodies were shown to be useful serotyping agents by correct identification in slide agglutination tests of 32 clinical isolates of all the S. typhi and other serogroup D salmonellae among a total of 140 bacterial isolates representing eight different Enterobacteriaceae genera tested.
Persistent Identifierhttp://hdl.handle.net/10722/172680
ISSN
2015 Impact Factor: 1.858
2015 SCImago Journal Rankings: 1.126

 

DC FieldValueLanguage
dc.contributor.authorLuk, JMCen_US
dc.contributor.authorZhao, CRen_US
dc.contributor.authorKarlsson, KMen_US
dc.contributor.authorLindberg, AAen_US
dc.date.accessioned2012-10-30T06:24:14Z-
dc.date.available2012-10-30T06:24:14Z-
dc.date.issued1992en_US
dc.identifier.citationFems Microbiology Letters, 1992, v. 97 n. 1-2, p. 173-178en_US
dc.identifier.issn0378-1097en_US
dc.identifier.urihttp://hdl.handle.net/10722/172680-
dc.description.abstractA panel of monoclonal antibodies were generated against the surface polysaccharide antigens of the cell envelope of Salmonella typhi. Four clones (IgM) were specific for the capsular Vi polysaccharide, and one clone (IgG3) reacted selectively with the S. typhi lipopolysaccharide in enzyme immunoassay. On the basis of their reactivity pattern and binding affinity, MATy-V7 (IgM) and MATy-O9 (IgG3) antibodies were selected for further characterization of their antigenic specificity. In an inhibition enzyme immunoassay with rabbit factor-specific anti-Salmonella antibodies as the competing agents, the reactivity of MATy-V7 and MATy-O9 were significantly inhibited by the anti-Vi and anti-O9 antisera, respectively. Moreover, both the Vi- and O9-specific monoclonal antibodies were shown to be useful serotyping agents by correct identification in slide agglutination tests of 32 clinical isolates of all the S. typhi and other serogroup D salmonellae among a total of 140 bacterial isolates representing eight different Enterobacteriaceae genera tested.en_US
dc.languageengen_US
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journal.asp?ref=0378-1097&site=1en_US
dc.relation.ispartofFEMS Microbiology Lettersen_US
dc.subjectLipopolysaccharideen_US
dc.subjectMonoclonal Antibodiesen_US
dc.subjectSalmonella Typhien_US
dc.subjectSerotypingen_US
dc.subjectVi Polysaccharideen_US
dc.titleSpecificity of monoclonal antibodies binding to the polysaccharide antigens (Vi, O9) of Salmonella typhien_US
dc.typeArticleen_US
dc.identifier.emailLuk, JMC: jmluk@hkucc.hku.hken_US
dc.identifier.authorityLuk, JMC=rp00349en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.scopuseid_2-s2.0-0026939196en_US
dc.identifier.volume97en_US
dc.identifier.issue1-2en_US
dc.identifier.spage173en_US
dc.identifier.epage178en_US
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridLuk, JMC=7006777791en_US
dc.identifier.scopusauthoridZhao, CR=36026129600en_US
dc.identifier.scopusauthoridKarlsson, KM=7201472561en_US
dc.identifier.scopusauthoridLindberg, AA=7101776377en_US

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