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Article: Murine monoclonal antibody specific for lipopolysaccharide of Salmonella serogroup A

TitleMurine monoclonal antibody specific for lipopolysaccharide of Salmonella serogroup A
Authors
Issue Date1987
Citation
Journal Of Clinical Microbiology, 1987, v. 25 n. 11, p. 2140-2144 How to Cite?
AbstractTo facilitate the routine identification of salmonellae and detailed studies of their lipopolysaccharides, we raised murine monoclonal antibodies against these organisms. We raised an immunoglobulin G1 antibody, MO2, which is specific for factor O2. By immunoblotting following sodium dodecyl sulfate-polyacrylamide gel electrophoresis, MO2 was shown to bind only the lipopolysaccharide of Salmonella paratyphi A, giving a ladderlike reaction pattern with regularly spaced reactive bands. MO2 did not react against lipopolysaccharides of other Salmonella serogroups, including those of serogroups B, C, D, E and L. Since the lipopolysaccharides of Salmonella serogroups A, B, D, and E are similar except for the presence of paratose in serogroup A organisms, this dideoxyhexose is therefore believed to be the immunodominant epitope for MO2. Consistent with the latter contention was the finding that periodate oxidation of the S. paratyphi A lipopolysaccharide did not destroy its antigenicity for MO2. In a slide agglutination test, MO2 was found to react specifically against all 12 clinical isolates of S. paratyphi A but not against 98 isolates of other salmonellae or 74 isolates of other bacteria and Candida albicans.
Persistent Identifierhttp://hdl.handle.net/10722/172562
ISSN
2015 Impact Factor: 3.631
2015 SCImago Journal Rankings: 2.151
PubMed Central ID
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DC FieldValueLanguage
dc.contributor.authorLuk, MCen_US
dc.contributor.authorTsang, RSWen_US
dc.contributor.authorNg, MHen_US
dc.date.accessioned2012-10-30T06:23:24Z-
dc.date.available2012-10-30T06:23:24Z-
dc.date.issued1987en_US
dc.identifier.citationJournal Of Clinical Microbiology, 1987, v. 25 n. 11, p. 2140-2144en_US
dc.identifier.issn0095-1137en_US
dc.identifier.urihttp://hdl.handle.net/10722/172562-
dc.description.abstractTo facilitate the routine identification of salmonellae and detailed studies of their lipopolysaccharides, we raised murine monoclonal antibodies against these organisms. We raised an immunoglobulin G1 antibody, MO2, which is specific for factor O2. By immunoblotting following sodium dodecyl sulfate-polyacrylamide gel electrophoresis, MO2 was shown to bind only the lipopolysaccharide of Salmonella paratyphi A, giving a ladderlike reaction pattern with regularly spaced reactive bands. MO2 did not react against lipopolysaccharides of other Salmonella serogroups, including those of serogroups B, C, D, E and L. Since the lipopolysaccharides of Salmonella serogroups A, B, D, and E are similar except for the presence of paratose in serogroup A organisms, this dideoxyhexose is therefore believed to be the immunodominant epitope for MO2. Consistent with the latter contention was the finding that periodate oxidation of the S. paratyphi A lipopolysaccharide did not destroy its antigenicity for MO2. In a slide agglutination test, MO2 was found to react specifically against all 12 clinical isolates of S. paratyphi A but not against 98 isolates of other salmonellae or 74 isolates of other bacteria and Candida albicans.en_US
dc.languageengen_US
dc.relation.ispartofJournal of Clinical Microbiologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodies, Monoclonal - Immunologyen_US
dc.subject.meshAntigens, Bacterial - Immunologyen_US
dc.subject.meshElectrophoresis, Polyacrylamide Gelen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_US
dc.subject.meshEpitopes - Analysis - Immunologyen_US
dc.subject.meshHybridomasen_US
dc.subject.meshImmunoassayen_US
dc.subject.meshLipopolysaccharides - Immunologyen_US
dc.subject.meshMiceen_US
dc.subject.meshO Antigensen_US
dc.subject.meshSalmonella Paratyphi A - Immunologyen_US
dc.titleMurine monoclonal antibody specific for lipopolysaccharide of Salmonella serogroup Aen_US
dc.typeArticleen_US
dc.identifier.emailLuk, MC: jmluk@hkucc.hku.hken_US
dc.identifier.authorityLuk, MC=rp00349en_US
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.pmid2447120-
dc.identifier.pmcidPMC269428-
dc.identifier.scopuseid_2-s2.0-0023270019en_US
dc.identifier.volume25en_US
dc.identifier.issue11en_US
dc.identifier.spage2140en_US
dc.identifier.epage2144en_US
dc.identifier.isiWOS:A1987K505800023-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLuk, MC=7006777791en_US
dc.identifier.scopusauthoridTsang, RSW=7102940066en_US
dc.identifier.scopusauthoridNg, MH=15035622000en_US

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