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Article: Increase of both circulating Th1 and Th2 T lymphocyte subsets in IgA nephropathy

TitleIncrease of both circulating Th1 and Th2 T lymphocyte subsets in IgA nephropathy
Authors
Keywordscytokine
IgA nephropathy
mRNA
Th1 cells
Th2 cells
Issue Date1994
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CEI
Citation
Clinical And Experimental Immunology, 1994, v. 96 n. 1, p. 116-121 How to Cite?
AbstractIgA nephropathy (IgAN), characterized by glomerular deposition of IgA and frequently elevated plasma IgA levels, has increased T helper cell activity. In vitro measurement of cytokines in supernatant of cultured peripheral lymphocytes revealed conflicting findings. We examined the profile of cytokine mRNA expressed in purified CD4+ cells in patients with IgAN in order to study their pattern of Th1 (releases IL-2 and interferon-gamma (IFN- γ)) and Th2 (releases IL-4 and IL-5) T cell response. We assessed the circulating CD4+ T cells in patients and normal controls by the expression of messenger RNA (mRNA) for IL-2, IL-4, IL-5 and IFN-γ. The cytokine mRNAs were analysed with reverse transcription-polymerase chain reaction and were measured semiquantitatively by using a housekeeping gene, β-actin. Compared with the control subjects, CD4+ T lymphocytes from patients with IgAN expressed a higher level of IL-2 mRNA (P = 0.007), IFN-γ mRNA (P = 0.04), IL-4 mRNA (P = 0.048), and IL-5 mRNA (P = 0.016). Within these patients with IgAN, a good correlation was demonstrated between the gene expression of cytokines in Th1 or Th2 cells. The IL-2 mRNA levels in Th1 cells from these patients with IgAN also correlated significantly with the IL-4 or IL-5 mRNA levels in their Th2 cells. Our study revealed IgAN is associated with activation in circulating lymphocytes of the IL-2, IFN-γ, IL-4 and IL-5 gene cluster, a pattern compatible with activation of both the Th1- and Th2-like T lymphocyte population. The increased transcription of these cytokine genes may be contributory to the immunopathologic findings in IgAN.
Persistent Identifierhttp://hdl.handle.net/10722/172005
ISSN
2015 Impact Factor: 3.148
2015 SCImago Journal Rankings: 1.369
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLai, KNen_HK
dc.contributor.authorHo, RTHen_HK
dc.contributor.authorLai, CKWen_HK
dc.contributor.authorChan, CHSen_HK
dc.contributor.authorLi, PKTen_HK
dc.date.accessioned2012-10-30T06:19:36Z-
dc.date.available2012-10-30T06:19:36Z-
dc.date.issued1994en_HK
dc.identifier.citationClinical And Experimental Immunology, 1994, v. 96 n. 1, p. 116-121en_HK
dc.identifier.issn0009-9104en_HK
dc.identifier.urihttp://hdl.handle.net/10722/172005-
dc.description.abstractIgA nephropathy (IgAN), characterized by glomerular deposition of IgA and frequently elevated plasma IgA levels, has increased T helper cell activity. In vitro measurement of cytokines in supernatant of cultured peripheral lymphocytes revealed conflicting findings. We examined the profile of cytokine mRNA expressed in purified CD4+ cells in patients with IgAN in order to study their pattern of Th1 (releases IL-2 and interferon-gamma (IFN- γ)) and Th2 (releases IL-4 and IL-5) T cell response. We assessed the circulating CD4+ T cells in patients and normal controls by the expression of messenger RNA (mRNA) for IL-2, IL-4, IL-5 and IFN-γ. The cytokine mRNAs were analysed with reverse transcription-polymerase chain reaction and were measured semiquantitatively by using a housekeeping gene, β-actin. Compared with the control subjects, CD4+ T lymphocytes from patients with IgAN expressed a higher level of IL-2 mRNA (P = 0.007), IFN-γ mRNA (P = 0.04), IL-4 mRNA (P = 0.048), and IL-5 mRNA (P = 0.016). Within these patients with IgAN, a good correlation was demonstrated between the gene expression of cytokines in Th1 or Th2 cells. The IL-2 mRNA levels in Th1 cells from these patients with IgAN also correlated significantly with the IL-4 or IL-5 mRNA levels in their Th2 cells. Our study revealed IgAN is associated with activation in circulating lymphocytes of the IL-2, IFN-γ, IL-4 and IL-5 gene cluster, a pattern compatible with activation of both the Th1- and Th2-like T lymphocyte population. The increased transcription of these cytokine genes may be contributory to the immunopathologic findings in IgAN.en_HK
dc.languageengen_US
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CEIen_HK
dc.relation.ispartofClinical and Experimental Immunologyen_HK
dc.subjectcytokineen_HK
dc.subjectIgA nephropathyen_HK
dc.subjectmRNAen_HK
dc.subjectTh1 cellsen_HK
dc.subjectTh2 cellsen_HK
dc.subject.meshBase Sequenceen_US
dc.subject.meshCd4-Positive T-Lymphocytes - Immunologyen_US
dc.subject.meshDna Primers - Chemistryen_US
dc.subject.meshFemaleen_US
dc.subject.meshGene Expressionen_US
dc.subject.meshGlomerulonephritis, Iga - Immunologyen_US
dc.subject.meshHumansen_US
dc.subject.meshInterferon-Gamma - Geneticsen_US
dc.subject.meshInterleukins - Geneticsen_US
dc.subject.meshMaleen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshRna, Messenger - Geneticsen_US
dc.subject.meshT-Lymphocyte Subsets - Immunologyen_US
dc.subject.meshT-Lymphocytes, Helper-Inducer - Immunologyen_US
dc.titleIncrease of both circulating Th1 and Th2 T lymphocyte subsets in IgA nephropathyen_HK
dc.typeArticleen_HK
dc.identifier.emailLai, KN: knlai@hku.hken_HK
dc.identifier.emailHo, RTH: tinho@hku.hken_HK
dc.identifier.authorityLai, KN=rp00324en_HK
dc.identifier.authorityHo, RTH=rp00497en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid7908616-
dc.identifier.scopuseid_2-s2.0-0028356379en_HK
dc.identifier.volume96en_HK
dc.identifier.issue1en_HK
dc.identifier.spage116en_HK
dc.identifier.epage121en_HK
dc.identifier.isiWOS:A1994ND72800021-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLai, KN=7402135706en_HK
dc.identifier.scopusauthoridHo, RTH=8620896500en_HK
dc.identifier.scopusauthoridLai, CKW=7403086390en_HK
dc.identifier.scopusauthoridChan, CHS=16169208100en_HK
dc.identifier.scopusauthoridLi, PKT=25928016800en_HK

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