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Article: Localization and characterization of 2[125I]iodomelatonin binding sites in female rat uterus

TitleLocalization and characterization of 2[125I]iodomelatonin binding sites in female rat uterus
Authors
Issue Date1998
PublisherFederation of American Societies for Experimental Biology. The Journal's web site is located at http://www.fasebj.org/
Citation
Faseb Journal, 1998, v. 12 n. 5, p. A686 How to Cite?
AbstractMelatonin plays an important role on the animal reproductive system, the hypothalamic-pituitary axis is considered as the site of melatonin action. However, recent studies have suggested a direct action of melatonin on gonads. Ih our laboratory, possible direct action of melatonin on the uterus was also investigated. Autoradiography was used to identify 2[125I]iodomelatonin binding sites in the ovary, oviduct and uterus of adult Sprague-Dawley rats. Positive binding was demonstrated in the uterus. Uterine binding mainly existed in the endometrium, no binding was demonstrated in the overy. Biochemical binding assay was carried out at 4°C, 21°C, 30°C and 37°C on the uterine membrance prepared from the whole uterine homogenates. The optimal condition for the assay was 30°C with maximum binding reached in 1.5 hours. A representative Kinetic study showed a dissociation constant (Kd) of 9.16 pmol/L. Scatchard analyses from uterus at difference stages of the estrous cycle indicated a Kd of 14.63 ± 2.84 pmol/L (n=l 1) and a maximum number of binding sites (Bmax) of 0.45 ± 0.08 fmol/mg protein (n=11). The number of uterine melatonin binding sites varied during the estrous cycle. Results suggest the presence of physiological relevant melatonin receptor in the rat uterine endometrium.
Persistent Identifierhttp://hdl.handle.net/10722/171762
ISSN
2015 Impact Factor: 5.299
2015 SCImago Journal Rankings: 2.775

 

DC FieldValueLanguage
dc.contributor.authorZhao, Hen_US
dc.contributor.authorPoon, AMSen_US
dc.contributor.authorPang, SFen_US
dc.date.accessioned2012-10-30T06:16:51Z-
dc.date.available2012-10-30T06:16:51Z-
dc.date.issued1998en_US
dc.identifier.citationFaseb Journal, 1998, v. 12 n. 5, p. A686en_US
dc.identifier.issn0892-6638en_US
dc.identifier.urihttp://hdl.handle.net/10722/171762-
dc.description.abstractMelatonin plays an important role on the animal reproductive system, the hypothalamic-pituitary axis is considered as the site of melatonin action. However, recent studies have suggested a direct action of melatonin on gonads. Ih our laboratory, possible direct action of melatonin on the uterus was also investigated. Autoradiography was used to identify 2[125I]iodomelatonin binding sites in the ovary, oviduct and uterus of adult Sprague-Dawley rats. Positive binding was demonstrated in the uterus. Uterine binding mainly existed in the endometrium, no binding was demonstrated in the overy. Biochemical binding assay was carried out at 4°C, 21°C, 30°C and 37°C on the uterine membrance prepared from the whole uterine homogenates. The optimal condition for the assay was 30°C with maximum binding reached in 1.5 hours. A representative Kinetic study showed a dissociation constant (Kd) of 9.16 pmol/L. Scatchard analyses from uterus at difference stages of the estrous cycle indicated a Kd of 14.63 ± 2.84 pmol/L (n=l 1) and a maximum number of binding sites (Bmax) of 0.45 ± 0.08 fmol/mg protein (n=11). The number of uterine melatonin binding sites varied during the estrous cycle. Results suggest the presence of physiological relevant melatonin receptor in the rat uterine endometrium.en_US
dc.languageengen_US
dc.publisherFederation of American Societies for Experimental Biology. The Journal's web site is located at http://www.fasebj.org/en_US
dc.relation.ispartofFASEB Journalen_US
dc.titleLocalization and characterization of 2[125I]iodomelatonin binding sites in female rat uterusen_US
dc.typeArticleen_US
dc.identifier.emailPoon, AMS:amspoon@hkucc.hku.hken_US
dc.identifier.authorityPoon, AMS=rp00354en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.scopuseid_2-s2.0-4243753795en_US
dc.identifier.volume12en_US
dc.identifier.issue5en_US
dc.identifier.spageA686en_US
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridZhao, H=14821083400en_US
dc.identifier.scopusauthoridPoon, AMS=7103068868en_US
dc.identifier.scopusauthoridPang, SF=7402528719en_US

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