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Article: Striatal neurons but not nigral dopaminergic neurons in neonatal primary cell culture express endogenous functional N-methyl-D-aspartate receptors

TitleStriatal neurons but not nigral dopaminergic neurons in neonatal primary cell culture express endogenous functional N-methyl-D-aspartate receptors
Authors
Issue Date2003
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/molbrainres
Citation
Molecular Brain Research, 2003, v. 120 n. 1, p. 9-12 How to Cite?
AbstractDevelopmental expression of N-methyl-D-aspartate (NMDA) receptor subunits were determined and compared in striatal and nigral neurons in neonatal primary cell cultures. In striatal neurons, NR1, NR2A and NR2B mRNAs and immunoreactivity, and NR2D mRNA were found and the maximal levels of NR1 mRNA and immunoreactivity expression were found at 6 day-in-vitro (DIV). NMDA receptors found at this stage in striatal neurons are likely to contain NR1 plus NR2A, NR2B and NR2D subunits. In nigral neurons, NR1 and NR2B mRNAs and immunoreactivity, and NR2D mRNA were found and the maximal level of NR1 immunoreactivity expression was found at 10 DIV. Unlike striatal neurons, NMDA receptors found in nigral neurons are likely to contain NR1 plus NR2B and NR2D subunits only. NMDA-induced toxicity assays showed that striatal neurons were most susceptible to cell death at around 10 DIV but nigral neurons were not susceptible to NMDA-induced cell death at all stages. In addition, patch clamp analysis revealed that functional NMDA receptors could only be found in striatal neurons but not in nigral dopaminergic neurons in vitro. The present results indicate that striatal and nigral neurons are programmed to express distinct NMDA receptor subunits during their endogenous development in cell cultures. Despite dopaminergic neurons in culture display NMDA receptor subunits, functional NMDA receptors are not assembled. The present findings have demonstrated that dopaminergic neurons in vitro may behave very differently to their counterparts in vivo in terms of NMDA receptor-mediated responses. Our results also have implications in transplantations using dopaminergic neurons in vitro in treatments of Parkinson's disease. © 2003 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/171721
ISSN
2007 Impact Factor: 1.997
2008 SCImago Journal Rankings: 1.457
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLui, PWen_US
dc.contributor.authorSuen, KCen_US
dc.contributor.authorChan, YSen_US
dc.contributor.authorYung, WHen_US
dc.contributor.authorYung, KKLen_US
dc.date.accessioned2012-10-30T06:16:36Z-
dc.date.available2012-10-30T06:16:36Z-
dc.date.issued2003en_US
dc.identifier.citationMolecular Brain Research, 2003, v. 120 n. 1, p. 9-12en_US
dc.identifier.issn0169-328Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/171721-
dc.description.abstractDevelopmental expression of N-methyl-D-aspartate (NMDA) receptor subunits were determined and compared in striatal and nigral neurons in neonatal primary cell cultures. In striatal neurons, NR1, NR2A and NR2B mRNAs and immunoreactivity, and NR2D mRNA were found and the maximal levels of NR1 mRNA and immunoreactivity expression were found at 6 day-in-vitro (DIV). NMDA receptors found at this stage in striatal neurons are likely to contain NR1 plus NR2A, NR2B and NR2D subunits. In nigral neurons, NR1 and NR2B mRNAs and immunoreactivity, and NR2D mRNA were found and the maximal level of NR1 immunoreactivity expression was found at 10 DIV. Unlike striatal neurons, NMDA receptors found in nigral neurons are likely to contain NR1 plus NR2B and NR2D subunits only. NMDA-induced toxicity assays showed that striatal neurons were most susceptible to cell death at around 10 DIV but nigral neurons were not susceptible to NMDA-induced cell death at all stages. In addition, patch clamp analysis revealed that functional NMDA receptors could only be found in striatal neurons but not in nigral dopaminergic neurons in vitro. The present results indicate that striatal and nigral neurons are programmed to express distinct NMDA receptor subunits during their endogenous development in cell cultures. Despite dopaminergic neurons in culture display NMDA receptor subunits, functional NMDA receptors are not assembled. The present findings have demonstrated that dopaminergic neurons in vitro may behave very differently to their counterparts in vivo in terms of NMDA receptor-mediated responses. Our results also have implications in transplantations using dopaminergic neurons in vitro in treatments of Parkinson's disease. © 2003 Elsevier B.V. All rights reserved.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/molbrainresen_US
dc.relation.ispartofMolecular Brain Researchen_US
dc.rightsMolecular Brain Research. Copyright © Elsevier BV.-
dc.subject.meshAnimalsen_US
dc.subject.meshAnimals, Newbornen_US
dc.subject.meshAntigens, Differentiation, Myelomonocytic - Pharmacologyen_US
dc.subject.meshCell Counten_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshDopamine - Metabolismen_US
dc.subject.meshFluorescent Antibody Technique - Methodsen_US
dc.subject.meshGlutamate Decarboxylase - Metabolismen_US
dc.subject.meshIsoenzymes - Metabolismen_US
dc.subject.meshMembrane Potentials - Drug Effectsen_US
dc.subject.meshNeostriatum - Cytology - Metabolismen_US
dc.subject.meshNeurons - Drug Effects - Metabolismen_US
dc.subject.meshPatch-Clamp Techniquesen_US
dc.subject.meshProtein Subunits - Genetics - Metabolismen_US
dc.subject.meshRna, Messenger - Biosynthesisen_US
dc.subject.meshRatsen_US
dc.subject.meshReceptors, N-Methyl-D-Aspartate - Genetics - Metabolismen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction - Methodsen_US
dc.subject.meshSubstantia Nigra - Cytology - Metabolismen_US
dc.subject.meshTime Factorsen_US
dc.subject.meshTranscription Factors - Pharmacologyen_US
dc.subject.meshTyrosine 3-Monooxygenase - Metabolismen_US
dc.titleStriatal neurons but not nigral dopaminergic neurons in neonatal primary cell culture express endogenous functional N-methyl-D-aspartate receptorsen_US
dc.typeArticleen_US
dc.identifier.emailChan, YS:yschan@hkucc.hku.hken_US
dc.identifier.authorityChan, YS=rp00318en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.molbrainres.2003.09.015en_US
dc.identifier.pmid14667572-
dc.identifier.scopuseid_2-s2.0-0344304806en_US
dc.identifier.hkuros92117-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0344304806&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume120en_US
dc.identifier.issue1en_US
dc.identifier.spage9en_US
dc.identifier.epage12en_US
dc.identifier.isiWOS:000187354200002-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridLui, PW=36916492100en_US
dc.identifier.scopusauthoridSuen, KC=8057602400en_US
dc.identifier.scopusauthoridChan, YS=7403676627en_US
dc.identifier.scopusauthoridYung, WH=7103137893en_US
dc.identifier.scopusauthoridYung, KKL=13605496000en_US

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