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Article: Localization of the cyclic ADP-ribose-dependent calcium signaling pathway in hepatocyte nucleus

TitleLocalization of the cyclic ADP-ribose-dependent calcium signaling pathway in hepatocyte nucleus
Authors
Issue Date2000
PublisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/
Citation
Journal Of Biological Chemistry, 2000, v. 275 n. 32, p. 24807-24817 How to Cite?
AbstractCD38 is a type II transmembrane glycoprotein found on both hematopoietic and non-hematopoietic cells. It is known for its involvement in the metabolism of cyclic ADP-ribose (cADPR) and nicotinic acid adenine dinucleotide phosphate, two nucleotides with calcium mobilizing activity independent of inositol trisphosphate. It is generally believed that CD38 is an integral protein with ectoenzymatic activities found mainly on the plasma membrane. Here we show that enzymatically active CD38 is present intracellularly on the nuclear envelope of rat hepatocytes. CD38 isolated from rat liver nuclei possessed both ADP-ribosyl cyclase and NADase activity. Immunofluorescence studies on rat liver cryosections and isolated nuclei localized CD38 to the nuclear envelope of hepatocytes. Subcellular localization via immunoelectron microscopy showed that CD38 is located on the inner nuclear envelope. The isolated nuclei sequestered calcium in an ATP-dependent manner. cADPR elicited a rapid calcium release from the loaded nuclei, which was independent of inositol trisphosphate and was inhibited by 8-amino-cADPR, a specific antagonist of cADPR, and ryanodine. However, nicotinic acid adenine dinucleotide phosphate failed to elicit any calcium release from the nuclear calcium stores. The nuclear localization of CD38 shown in this study suggests a novel role of CD38 in intracellular calcium signaling for non-hematopoietic cells.
Persistent Identifierhttp://hdl.handle.net/10722/171687
ISSN
2015 Impact Factor: 4.258
2015 SCImago Journal Rankings: 3.151
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorKeng Meng Khooen_US
dc.contributor.authorHan, MKen_US
dc.contributor.authorPark, JBen_US
dc.contributor.authorSoo Wan Chaeen_US
dc.contributor.authorKim, UHen_US
dc.contributor.authorHon Cheung Leeen_US
dc.contributor.authorBoon Huat Bayen_US
dc.contributor.authorChan Fong Changen_US
dc.date.accessioned2012-10-30T06:16:21Z-
dc.date.available2012-10-30T06:16:21Z-
dc.date.issued2000en_US
dc.identifier.citationJournal Of Biological Chemistry, 2000, v. 275 n. 32, p. 24807-24817en_US
dc.identifier.issn0021-9258en_US
dc.identifier.urihttp://hdl.handle.net/10722/171687-
dc.description.abstractCD38 is a type II transmembrane glycoprotein found on both hematopoietic and non-hematopoietic cells. It is known for its involvement in the metabolism of cyclic ADP-ribose (cADPR) and nicotinic acid adenine dinucleotide phosphate, two nucleotides with calcium mobilizing activity independent of inositol trisphosphate. It is generally believed that CD38 is an integral protein with ectoenzymatic activities found mainly on the plasma membrane. Here we show that enzymatically active CD38 is present intracellularly on the nuclear envelope of rat hepatocytes. CD38 isolated from rat liver nuclei possessed both ADP-ribosyl cyclase and NADase activity. Immunofluorescence studies on rat liver cryosections and isolated nuclei localized CD38 to the nuclear envelope of hepatocytes. Subcellular localization via immunoelectron microscopy showed that CD38 is located on the inner nuclear envelope. The isolated nuclei sequestered calcium in an ATP-dependent manner. cADPR elicited a rapid calcium release from the loaded nuclei, which was independent of inositol trisphosphate and was inhibited by 8-amino-cADPR, a specific antagonist of cADPR, and ryanodine. However, nicotinic acid adenine dinucleotide phosphate failed to elicit any calcium release from the nuclear calcium stores. The nuclear localization of CD38 shown in this study suggests a novel role of CD38 in intracellular calcium signaling for non-hematopoietic cells.en_US
dc.languageengen_US
dc.publisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/en_US
dc.relation.ispartofJournal of Biological Chemistryen_US
dc.subject.meshAdp-Ribosyl Cyclaseen_US
dc.subject.meshAdenosine Diphosphate Ribose - Analogs & Derivatives - Metabolismen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntigens, Cden_US
dc.subject.meshAntigens, Cd38en_US
dc.subject.meshAntigens, Differentiation - Analysis - Isolation & Purification - Metabolismen_US
dc.subject.meshCalcium Signaling - Physiologyen_US
dc.subject.meshCell Nucleus - Metabolism - Ultrastructureen_US
dc.subject.meshCyclic Adp-Riboseen_US
dc.subject.meshKineticsen_US
dc.subject.meshLiver - Metabolism - Ultrastructureen_US
dc.subject.meshMembrane Glycoproteinsen_US
dc.subject.meshMicroscopy, Immunoelectronen_US
dc.subject.meshMultienzyme Complexes - Metabolismen_US
dc.subject.meshNad+ Nucleosidase - Analysis - Isolation & Purification - Metabolismen_US
dc.subject.meshNadp - Metabolismen_US
dc.subject.meshNuclear Envelope - Enzymology - Ultrastructureen_US
dc.subject.meshRatsen_US
dc.titleLocalization of the cyclic ADP-ribose-dependent calcium signaling pathway in hepatocyte nucleusen_US
dc.typeArticleen_US
dc.identifier.emailHon Cheung Lee:leehc@hku.hken_US
dc.identifier.authorityHon Cheung Lee=rp00545en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1074/jbc.M908231199en_US
dc.identifier.pmid10818108-
dc.identifier.scopuseid_2-s2.0-0034637449en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034637449&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume275en_US
dc.identifier.issue32en_US
dc.identifier.spage24807en_US
dc.identifier.epage24817en_US
dc.identifier.isiWOS:000088683300078-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridKeng Meng Khoo=7409772892en_US
dc.identifier.scopusauthoridHan, MK=7402603749en_US
dc.identifier.scopusauthoridPark, JB=8838858000en_US
dc.identifier.scopusauthoridSoo Wan Chae=7409688124en_US
dc.identifier.scopusauthoridKim, UH=7102248950en_US
dc.identifier.scopusauthoridHon Cheung Lee=26642959100en_US
dc.identifier.scopusauthoridBoon Huat Bay=7409950986en_US
dc.identifier.scopusauthoridChan Fong Chang=7409693362en_US

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