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Article: The effect of lactic acid on intracellular pH and adenosine output from superfused rat soleus muscle fibres

TitleThe effect of lactic acid on intracellular pH and adenosine output from superfused rat soleus muscle fibres
Authors
Issue Date2000
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescie
Citation
Life Sciences, 2000, v. 67 n. 3, p. 227-234 How to Cite?
AbstractWe investigated the effects of graded doses of lactic acid on the intracellular pH and adenosine output from superfused bundles of about 15 skeletal muscle fibres. Intracellular pH was determined using the fluorescent intracellular dye, 2',7'-bis-(2-carboxyethyl)-5-(and -6-) carboxyfluorescein (BCECF), and adenosine efflux was measured by HPLC. Intracellular pH was 7.07 ± 0.05 under control conditions, which was around 0.35 units lower than extracellular pH, and adenosine output was 63 ± 10 pmol/min/g. Lactic acid produced dose-dependent decreases in intracellular pH and dose-dependent increases in adenosine output: 10 mM lactic acid decreased intracellular pH to 6.57 ± 0.04 and increased adenosine output to 159 ± 34 pmol/min/g. The adenosine output and the intracellular pH were well correlated (r2 = 0.988; P < 0,01). (C) 2000 Elsevier Science Inc.
Persistent Identifierhttp://hdl.handle.net/10722/171685
ISSN
2015 Impact Factor: 2.685
2015 SCImago Journal Rankings: 1.056
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorMo, FMen_US
dc.contributor.authorBallard, HJen_US
dc.date.accessioned2012-10-30T06:16:21Z-
dc.date.available2012-10-30T06:16:21Z-
dc.date.issued2000en_US
dc.identifier.citationLife Sciences, 2000, v. 67 n. 3, p. 227-234en_US
dc.identifier.issn0024-3205en_US
dc.identifier.urihttp://hdl.handle.net/10722/171685-
dc.description.abstractWe investigated the effects of graded doses of lactic acid on the intracellular pH and adenosine output from superfused bundles of about 15 skeletal muscle fibres. Intracellular pH was determined using the fluorescent intracellular dye, 2',7'-bis-(2-carboxyethyl)-5-(and -6-) carboxyfluorescein (BCECF), and adenosine efflux was measured by HPLC. Intracellular pH was 7.07 ± 0.05 under control conditions, which was around 0.35 units lower than extracellular pH, and adenosine output was 63 ± 10 pmol/min/g. Lactic acid produced dose-dependent decreases in intracellular pH and dose-dependent increases in adenosine output: 10 mM lactic acid decreased intracellular pH to 6.57 ± 0.04 and increased adenosine output to 159 ± 34 pmol/min/g. The adenosine output and the intracellular pH were well correlated (r2 = 0.988; P < 0,01). (C) 2000 Elsevier Science Inc.en_US
dc.languageengen_US
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescieen_US
dc.relation.ispartofLife Sciencesen_US
dc.subject.meshAdenosine - Metabolismen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBuffersen_US
dc.subject.meshCarbon Dioxide - Metabolismen_US
dc.subject.meshChromatography, High Pressure Liquiden_US
dc.subject.meshDose-Response Relationship, Drugen_US
dc.subject.meshFluoresceinsen_US
dc.subject.meshFluorescent Dyesen_US
dc.subject.meshHydrogen-Ion Concentrationen_US
dc.subject.meshLactic Acid - Pharmacologyen_US
dc.subject.meshMaleen_US
dc.subject.meshMuscle Fibers, Fast-Twitch - Drug Effects - Metabolismen_US
dc.subject.meshMuscle, Skeletal - Drug Effects - Metabolismen_US
dc.subject.meshOxygen - Metabolismen_US
dc.subject.meshPartial Pressureen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.titleThe effect of lactic acid on intracellular pH and adenosine output from superfused rat soleus muscle fibresen_US
dc.typeArticleen_US
dc.identifier.emailBallard, HJ:ballard@hkucc.hku.hken_US
dc.identifier.authorityBallard, HJ=rp00367en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/S0024-3205(00)00624-Xen_US
dc.identifier.pmid10983866-
dc.identifier.scopuseid_2-s2.0-0034625773en_US
dc.identifier.hkuros53127-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034625773&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume67en_US
dc.identifier.issue3en_US
dc.identifier.spage227en_US
dc.identifier.epage234en_US
dc.identifier.isiWOS:000087709700002-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridMo, FM=7005059536en_US
dc.identifier.scopusauthoridBallard, HJ=7005286310en_US

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