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Article: Two distinct pathways for refilling Ca2+ stores in permeabilized bovine trachealis muscle

TitleTwo distinct pathways for refilling Ca2+ stores in permeabilized bovine trachealis muscle
Authors
Issue Date1999
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescie
Citation
Life Sciences, 1999, v. 64 n. 22, p. 2049-2059 How to Cite?
AbstractCalcium entry from extracellular space to acetylcholine (ACh)-sensitive internal stores was investigated in β-escin permeabilized bovine tracheal smooth muscle. Cyclopiazonic acid (CPA), a selective inhibitor of the sarcoplasmic reticulum (SR) calcium pump, and nifedipine, both inhibited the refilling, and inhibition was larger when these compounds were used simultaneously. BayK 8644 enhanced the refilling and completely reversed the inhibition induced by cyclopiazonic acid. In pCa 7 solution containing CPA, there was a spontaneous time-dependent decrease of ACh-induced transient contraction. In the presence of nifedipine or verapamil in the incubation solution reduced this time-dependent decrease in contractile responses to ACh stimulation, suggesting that these calcium-entry blockers decreased calcium leakage from internal stores to the extracellular space. These results suggest that in addition to the active calcium uptake in the SR, another pathway controlled by an L-type like calcium channel (dihydropyridine- sensitive) may exist between the extracellular compartment and the lumen of the SR in airway smooth muscle, and contributes significantly to the loading of ACh-sensitive calcium stores.
Persistent Identifierhttp://hdl.handle.net/10722/171672
ISSN
2015 Impact Factor: 2.685
2015 SCImago Journal Rankings: 1.056
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorQian, Yen_US
dc.contributor.authorBourreau, JPen_US
dc.date.accessioned2012-10-30T06:16:16Z-
dc.date.available2012-10-30T06:16:16Z-
dc.date.issued1999en_US
dc.identifier.citationLife Sciences, 1999, v. 64 n. 22, p. 2049-2059en_US
dc.identifier.issn0024-3205en_US
dc.identifier.urihttp://hdl.handle.net/10722/171672-
dc.description.abstractCalcium entry from extracellular space to acetylcholine (ACh)-sensitive internal stores was investigated in β-escin permeabilized bovine tracheal smooth muscle. Cyclopiazonic acid (CPA), a selective inhibitor of the sarcoplasmic reticulum (SR) calcium pump, and nifedipine, both inhibited the refilling, and inhibition was larger when these compounds were used simultaneously. BayK 8644 enhanced the refilling and completely reversed the inhibition induced by cyclopiazonic acid. In pCa 7 solution containing CPA, there was a spontaneous time-dependent decrease of ACh-induced transient contraction. In the presence of nifedipine or verapamil in the incubation solution reduced this time-dependent decrease in contractile responses to ACh stimulation, suggesting that these calcium-entry blockers decreased calcium leakage from internal stores to the extracellular space. These results suggest that in addition to the active calcium uptake in the SR, another pathway controlled by an L-type like calcium channel (dihydropyridine- sensitive) may exist between the extracellular compartment and the lumen of the SR in airway smooth muscle, and contributes significantly to the loading of ACh-sensitive calcium stores.en_US
dc.languageengen_US
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescieen_US
dc.relation.ispartofLife Sciencesen_US
dc.rightsLife Sciences. Copyright © Elsevier Inc.-
dc.subject.meshAcetylcholine - Pharmacologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCalcium - Metabolismen_US
dc.subject.meshCalcium Channel Blockers - Pharmacologyen_US
dc.subject.meshCalcium Channels - Metabolismen_US
dc.subject.meshCalcium Signaling - Drug Effectsen_US
dc.subject.meshCalcium-Transporting Atpases - Antagonists & Inhibitorsen_US
dc.subject.meshCattleen_US
dc.subject.meshCell Membrane Permeability - Drug Effectsen_US
dc.subject.meshEnzyme Inhibitors - Pharmacologyen_US
dc.subject.meshEscin - Pharmacologyen_US
dc.subject.meshIndoles - Pharmacologyen_US
dc.subject.meshIon Transport - Drug Effectsen_US
dc.subject.meshMuscle Contraction - Drug Effectsen_US
dc.subject.meshMuscle, Smooth - Drug Effects - Metabolism - Physiologyen_US
dc.subject.meshNifedipine - Pharmacologyen_US
dc.subject.meshSarcoplasmic Reticulum - Drug Effects - Metabolismen_US
dc.subject.meshTrachea - Drug Effects - Metabolism - Physiologyen_US
dc.subject.meshVerapamil - Pharmacologyen_US
dc.titleTwo distinct pathways for refilling Ca2+ stores in permeabilized bovine trachealis muscleen_US
dc.typeArticleen_US
dc.identifier.emailBourreau, JP:bourreau@hkucc.hku.hken_US
dc.identifier.authorityBourreau, JP=rp00389en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/S0024-3205(99)00152-6en_US
dc.identifier.pmid10374930-
dc.identifier.scopuseid_2-s2.0-0033597342en_US
dc.identifier.hkuros44391-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033597342&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume64en_US
dc.identifier.issue22en_US
dc.identifier.spage2049en_US
dc.identifier.epage2059en_US
dc.identifier.isiWOS:000080107200009-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridQian, Y=37019487300en_US
dc.identifier.scopusauthoridBourreau, JP=7003927886en_US

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