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Article: Effect of melatonin on chloride secretion by human colonic T84 cells

TitleEffect of melatonin on chloride secretion by human colonic T84 cells
Authors
KeywordsCl - Secretion
Melatonin
T 84 Cells
Issue Date1998
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescie
Citation
Life Sciences, 1998, v. 62 n. 23, p. 2151-2158 How to Cite?
AbstractThe effect of melatonin on human colonic T84 cells was studied using the short-circuit current (I(SC)) technique. Basolateral, as well as apical, addition of melatonin stimulated I(SC) in a concentration-dependent manner (EC 50 at about 100 μM). The I(SC) response to melatonin was nearly abolished when external Cl - was removed. The increase in I(SC) was also blocked by apical addition of two Cl - channel blockers, 4,4'-diisothiocyanatostibene-2,2'-disulfonic acid (DIDS) and diphenylamine 2-carboxylic acid (DPC), indicating that melatonin stimulated Cl - secretion. The effect of different melatonin analogs was compared and the order of potency appeased to be 2-iodomelatonin > melatonin > 6-hydroxymelatonin, indicating the involvement of melatonin receptors. However, inhibitors for G(i)-protein, adenylate cyclase or phospholipase C were found to be ineffective in inhibiting the melatonin-induced I(SC). Pretreatment of the cells with melatonin was also found to exert little effect on subsequent forskolin- or VIP-induced I(SC), further excluding its interaction with adenylate cyclase. Our data suggest that melatonin may play a role in regulating colonic Cl - secretion via melatonin receptors; however, the signal transduction pathway(s) involved remains to be elucidated.
Persistent Identifierhttp://hdl.handle.net/10722/171646
ISSN
2015 Impact Factor: 2.685
2015 SCImago Journal Rankings: 1.056
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChan, HCen_US
dc.contributor.authorLui, KMen_US
dc.contributor.authorWong, WSen_US
dc.contributor.authorPoon, AMSen_US
dc.date.accessioned2012-10-30T06:16:08Z-
dc.date.available2012-10-30T06:16:08Z-
dc.date.issued1998en_US
dc.identifier.citationLife Sciences, 1998, v. 62 n. 23, p. 2151-2158en_US
dc.identifier.issn0024-3205en_US
dc.identifier.urihttp://hdl.handle.net/10722/171646-
dc.description.abstractThe effect of melatonin on human colonic T84 cells was studied using the short-circuit current (I(SC)) technique. Basolateral, as well as apical, addition of melatonin stimulated I(SC) in a concentration-dependent manner (EC 50 at about 100 μM). The I(SC) response to melatonin was nearly abolished when external Cl - was removed. The increase in I(SC) was also blocked by apical addition of two Cl - channel blockers, 4,4'-diisothiocyanatostibene-2,2'-disulfonic acid (DIDS) and diphenylamine 2-carboxylic acid (DPC), indicating that melatonin stimulated Cl - secretion. The effect of different melatonin analogs was compared and the order of potency appeased to be 2-iodomelatonin > melatonin > 6-hydroxymelatonin, indicating the involvement of melatonin receptors. However, inhibitors for G(i)-protein, adenylate cyclase or phospholipase C were found to be ineffective in inhibiting the melatonin-induced I(SC). Pretreatment of the cells with melatonin was also found to exert little effect on subsequent forskolin- or VIP-induced I(SC), further excluding its interaction with adenylate cyclase. Our data suggest that melatonin may play a role in regulating colonic Cl - secretion via melatonin receptors; however, the signal transduction pathway(s) involved remains to be elucidated.en_US
dc.languageengen_US
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescieen_US
dc.relation.ispartofLife Sciencesen_US
dc.subjectCl - Secretionen_US
dc.subjectMelatoninen_US
dc.subjectT 84 Cellsen_US
dc.titleEffect of melatonin on chloride secretion by human colonic T84 cellsen_US
dc.typeArticleen_US
dc.identifier.emailPoon, AMS:amspoon@hkucc.hku.hken_US
dc.identifier.authorityPoon, AMS=rp00354en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/S0024-3205(98)00190-8en_US
dc.identifier.pmid9627094-
dc.identifier.scopuseid_2-s2.0-0031637408en_US
dc.identifier.hkuros36963-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031637408&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume62en_US
dc.identifier.issue23en_US
dc.identifier.spage2151en_US
dc.identifier.epage2158en_US
dc.identifier.isiWOS:000073527200009-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridChan, HC=7403402737en_US
dc.identifier.scopusauthoridLui, KM=7103389986en_US
dc.identifier.scopusauthoridWong, WS=7403972688en_US
dc.identifier.scopusauthoridPoon, AMS=7103068868en_US
dc.customcontrol.immutablecsl 151204-

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