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Article: Evidence for a direct action of melatonin in the immune system

TitleEvidence for a direct action of melatonin in the immune system
Authors
Issue Date1994
PublisherS Karger AG. The Journal's web site is located at http://www.karger.com/NSG
Citation
Biological Signals, 1994, v. 3 n. 2, p. 107-117 How to Cite?
AbstractPineal melatoninin modulates the mammalian immune system. In vivo studies showed that melatonin enhanced the natural and acquired immunity while in vitro studies demonstrated its inhibitory influence. The mechanism of melatonin action on the immune system remains unknown. Actions through lymphokines or opioid release or via other endocrine changes have been proposed. In this paper, a direct action of melatonin on the lymphoid tissue is hypothesized. 2-[125I]Iodomelatonin binding sites have been identified in the membrane homogenates of thymus, bursa of Fabricius and spleens of a number of birds and mammals. The bindings were stable, saturable, reversible, specific and of high affinity. The Bmax ranged from 0.6 to 3.9 fmol/mg protein. The Kd was in the physiologial range of circulating melatonin levels, about 30-70 pmol/l. The binding sites in the primary lymphoid organs demonstrated diurnal variation in density, with higher levels found at the middle of the light period. However, those in the spleen did not vary with the time of the day. An age-dependent decrease in the density was also found in the chicken bursa of Fabricius. In addition, when the noctural melatonin secretion was suppressed by constant light exposure, the density of the binding sites increased in the guinea pig spleen. Immunosuppression with cortisol injection in young ducks decreased the density of the melatonin binding sites in the thymus. The regulation of the binding characteristics by physiological variation in melatonin levels and/or immunological status of the animals provide evidence that these 2-[125I]iodomelatonin binding sites in the lymphoid tissues may be physiologically significant and represent true melatonin receptors. The melatonin receptors in the lymphoid organs may be coupled to a G protein as Guanosine 5'-0-(3-thiotriphosphate inhibited 2-[125I]iodomelatonin binding in the spleen by increasing the Kd and decreasing the Bmax.
Persistent Identifierhttp://hdl.handle.net/10722/171611
ISSN

 

DC FieldValueLanguage
dc.contributor.authorPoon, AMSen_US
dc.contributor.authorLiu, ZMen_US
dc.contributor.authorPang, CSen_US
dc.contributor.authorBrown, GMen_US
dc.contributor.authorPang, SFen_US
dc.date.accessioned2012-10-30T06:15:57Z-
dc.date.available2012-10-30T06:15:57Z-
dc.date.issued1994en_US
dc.identifier.citationBiological Signals, 1994, v. 3 n. 2, p. 107-117en_US
dc.identifier.issn1016-0922en_US
dc.identifier.urihttp://hdl.handle.net/10722/171611-
dc.description.abstractPineal melatoninin modulates the mammalian immune system. In vivo studies showed that melatonin enhanced the natural and acquired immunity while in vitro studies demonstrated its inhibitory influence. The mechanism of melatonin action on the immune system remains unknown. Actions through lymphokines or opioid release or via other endocrine changes have been proposed. In this paper, a direct action of melatonin on the lymphoid tissue is hypothesized. 2-[125I]Iodomelatonin binding sites have been identified in the membrane homogenates of thymus, bursa of Fabricius and spleens of a number of birds and mammals. The bindings were stable, saturable, reversible, specific and of high affinity. The Bmax ranged from 0.6 to 3.9 fmol/mg protein. The Kd was in the physiologial range of circulating melatonin levels, about 30-70 pmol/l. The binding sites in the primary lymphoid organs demonstrated diurnal variation in density, with higher levels found at the middle of the light period. However, those in the spleen did not vary with the time of the day. An age-dependent decrease in the density was also found in the chicken bursa of Fabricius. In addition, when the noctural melatonin secretion was suppressed by constant light exposure, the density of the binding sites increased in the guinea pig spleen. Immunosuppression with cortisol injection in young ducks decreased the density of the melatonin binding sites in the thymus. The regulation of the binding characteristics by physiological variation in melatonin levels and/or immunological status of the animals provide evidence that these 2-[125I]iodomelatonin binding sites in the lymphoid tissues may be physiologically significant and represent true melatonin receptors. The melatonin receptors in the lymphoid organs may be coupled to a G protein as Guanosine 5'-0-(3-thiotriphosphate inhibited 2-[125I]iodomelatonin binding in the spleen by increasing the Kd and decreasing the Bmax.en_US
dc.languageengen_US
dc.publisherS Karger AG. The Journal's web site is located at http://www.karger.com/NSGen_US
dc.relation.ispartofBiological Signalsen_US
dc.rightsBiological Signals. Copyright © S Karger AG.-
dc.subject.meshAnimalsen_US
dc.subject.meshBirds - Physiologyen_US
dc.subject.meshGtp-Binding Proteins - Physiologyen_US
dc.subject.meshHumansen_US
dc.subject.meshImmune System - Physiologyen_US
dc.subject.meshImmunosuppressive Agents - Pharmacologyen_US
dc.subject.meshLymphoid Tissue - Metabolismen_US
dc.subject.meshMammals - Physiologyen_US
dc.subject.meshMelatonin - Analogs & Derivatives - Metabolism - Physiologyen_US
dc.subject.meshNeuroimmunomodulation - Physiologyen_US
dc.subject.meshNeurotransmitter Agents - Metabolismen_US
dc.subject.meshReceptors, Cell Surface - Metabolismen_US
dc.subject.meshReceptors, Melatoninen_US
dc.subject.meshSignal Transductionen_US
dc.subject.meshSpecies Specificityen_US
dc.titleEvidence for a direct action of melatonin in the immune systemen_US
dc.typeArticleen_US
dc.identifier.emailPoon, AMS:amspoon@hkucc.hku.hken_US
dc.identifier.emailPang, SF: hrmypsf@hkucc.hku.hk-
dc.identifier.authorityPoon, AMS=rp00354en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid7951648-
dc.identifier.scopuseid_2-s2.0-0028336744en_US
dc.identifier.hkuros2685-
dc.identifier.volume3en_US
dc.identifier.issue2en_US
dc.identifier.spage107en_US
dc.identifier.epage117en_US
dc.publisher.placeSwitzerlanden_US
dc.identifier.scopusauthoridPoon, AMS=7103068868en_US
dc.identifier.scopusauthoridLiu, ZM=7406671362en_US
dc.identifier.scopusauthoridPang, CS=7201425191en_US
dc.identifier.scopusauthoridBrown, GM=35493704500en_US
dc.identifier.scopusauthoridPang, SF=7402528719en_US

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