File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Putative melatonin receptors in the male guinea pig kidney.

TitlePutative melatonin receptors in the male guinea pig kidney.
Authors
Issue Date1993
PublisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JPI
Citation
Journal Of Pineal Research, 1993, v. 15 n. 3, p. 153-160 How to Cite?
AbstractThe direct action of pineal melatonin on the renal system is supported by our demonstration of 2-[125I]iodomelatonin binding sites in the male guinea pig kidney. Scatchard analyses and Hill coefficients revealed a single type of binding site with an equilibrium dissociation constant (Kd) of 22.3 +/- 1.6 pmol/l and a maximum binding density (Bmax) of 0.99 +/- 0.03 fmol/mg protein (n = 7) at mid-light. There was no significant difference in the Kd and Bmax values between kidney tissues collected at the middle of light and dark periods. The pharmacological profile of these 2-[125I]iodomelatonin binding sites indicated high specificity for melatonin, 2-iodomelatonin and 6-chloromelatonin while kinetic studies generated a Kd value of 28.4 +/- 7.3 pmol/l (n = 5) which was comparable to that determined from Scatchard transformations. Our results suggest that these binding sites are stable, reversible, saturable, specific, and of high affinity. Regional distribution study showed that specific binding of 2-[125I]iodomelatonin was 8-fold higher in the cortical region than that in the medullary region. Studies of subcellular distribution showed that 59.3% of binding sites were localized in crude nuclear fractions followed by crude mitochondrial fractions (22.3%) and crude microsomal fractions (18.3%) with no detectable binding in cytosolic fractions. Our present findings suggest the presence of putative melatonin receptors in the guinea pig kidney, which support the hypotheses of melatonin-regulated renin secretion together with renal excretory functions via melatonin receptors.
Persistent Identifierhttp://hdl.handle.net/10722/171596
ISSN
2015 Impact Factor: 9.314
2015 SCImago Journal Rankings: 2.655
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorSong, Yen_US
dc.contributor.authorPoon, AMen_US
dc.contributor.authorLee, PPen_US
dc.contributor.authorPang, SFen_US
dc.date.accessioned2012-10-30T06:15:54Z-
dc.date.available2012-10-30T06:15:54Z-
dc.date.issued1993en_US
dc.identifier.citationJournal Of Pineal Research, 1993, v. 15 n. 3, p. 153-160en_US
dc.identifier.issn0742-3098en_US
dc.identifier.urihttp://hdl.handle.net/10722/171596-
dc.description.abstractThe direct action of pineal melatonin on the renal system is supported by our demonstration of 2-[125I]iodomelatonin binding sites in the male guinea pig kidney. Scatchard analyses and Hill coefficients revealed a single type of binding site with an equilibrium dissociation constant (Kd) of 22.3 +/- 1.6 pmol/l and a maximum binding density (Bmax) of 0.99 +/- 0.03 fmol/mg protein (n = 7) at mid-light. There was no significant difference in the Kd and Bmax values between kidney tissues collected at the middle of light and dark periods. The pharmacological profile of these 2-[125I]iodomelatonin binding sites indicated high specificity for melatonin, 2-iodomelatonin and 6-chloromelatonin while kinetic studies generated a Kd value of 28.4 +/- 7.3 pmol/l (n = 5) which was comparable to that determined from Scatchard transformations. Our results suggest that these binding sites are stable, reversible, saturable, specific, and of high affinity. Regional distribution study showed that specific binding of 2-[125I]iodomelatonin was 8-fold higher in the cortical region than that in the medullary region. Studies of subcellular distribution showed that 59.3% of binding sites were localized in crude nuclear fractions followed by crude mitochondrial fractions (22.3%) and crude microsomal fractions (18.3%) with no detectable binding in cytosolic fractions. Our present findings suggest the presence of putative melatonin receptors in the guinea pig kidney, which support the hypotheses of melatonin-regulated renin secretion together with renal excretory functions via melatonin receptors.en_US
dc.languageengen_US
dc.publisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JPIen_US
dc.relation.ispartofJournal of pineal researchen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBinding Sitesen_US
dc.subject.meshGuinea Pigsen_US
dc.subject.meshKidney Cortex - Metabolismen_US
dc.subject.meshKidney Medulla - Metabolismen_US
dc.subject.meshMaleen_US
dc.subject.meshMelatonin - Analogs & Derivatives - Blood - Metabolismen_US
dc.subject.meshRadioimmunoassayen_US
dc.subject.meshReceptors, Cell Surface - Metabolismen_US
dc.subject.meshReceptors, Melatoninen_US
dc.subject.meshSubcellular Fractions - Metabolismen_US
dc.titlePutative melatonin receptors in the male guinea pig kidney.en_US
dc.typeArticleen_US
dc.identifier.emailPoon, AM:amspoon@hkucc.hku.hken_US
dc.identifier.authorityPoon, AM=rp00354en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1111/j.1600-079X.1993.tb00523.x-
dc.identifier.pmid8106962-
dc.identifier.scopuseid_2-s2.0-0027673693en_US
dc.identifier.volume15en_US
dc.identifier.issue3en_US
dc.identifier.spage153en_US
dc.identifier.epage160en_US
dc.identifier.isiWOS:A1993MM94600007-
dc.publisher.placeDenmarken_US
dc.identifier.scopusauthoridSong, Y=7404920869en_US
dc.identifier.scopusauthoridPoon, AM=7103068868en_US
dc.identifier.scopusauthoridLee, PP=7406119629en_US
dc.identifier.scopusauthoridPang, SF=7402528719en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats