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Article: Chloride and depolarization by acetylcholine in canine airway smooth muscle

TitleChloride and depolarization by acetylcholine in canine airway smooth muscle
Authors
Issue Date1993
PublisherN R C Research Press. The Journal's web site is located at http://pubs.nrc-cnrc.gc.ca/cgi-bin/rp/rp2_desc_e?cjpp
Citation
Canadian Journal Of Physiology And Pharmacology, 1993, v. 71 n. 3-4, p. 284-292 How to Cite?
AbstractThe role of chloride channels has been examined in canine tracheal smooth muscle by recording mechanical responses to field stimulation and to acetylcholine (ACh) and by sucrose gap recording of excitatory junction potentials and ACh-induced electrical changes. The results of substitution studies using isethionate for chloride provided evidence that a chloride conductance contributes to the resting potential. The extrapolated reversal potential for ACh-induced depolarization was positive to the resting potential. Isethionate substitution inhibited ACh-induced depolarization, consistent with a contribution from increased Cl- conductance to the depolarization induced by ACh. However, closure of K+ channels and opening of a nonspecific cation channel could also contribute to depolarization. Further study of the effects of isethionate substitution during prolonged tissue exposure to chloride-free medium showed that retention or the accumulation of Ca2+ in intracellular stores was impaired. We conclude that effects of chloride deprivation on responses to ACh may reflect an early increase in Cl- conductance, but longer term changes reflect the requirement for this anion to maintain internal Ca2+ stores.
Persistent Identifierhttp://hdl.handle.net/10722/171573
ISSN
2015 Impact Factor: 1.704
2015 SCImago Journal Rankings: 0.683
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorDaniel, EEen_US
dc.contributor.authorJury, Jen_US
dc.contributor.authorBourreau, JPen_US
dc.contributor.authorJager, Len_US
dc.date.accessioned2012-10-30T06:15:45Z-
dc.date.available2012-10-30T06:15:45Z-
dc.date.issued1993en_US
dc.identifier.citationCanadian Journal Of Physiology And Pharmacology, 1993, v. 71 n. 3-4, p. 284-292en_US
dc.identifier.issn0008-4212en_US
dc.identifier.urihttp://hdl.handle.net/10722/171573-
dc.description.abstractThe role of chloride channels has been examined in canine tracheal smooth muscle by recording mechanical responses to field stimulation and to acetylcholine (ACh) and by sucrose gap recording of excitatory junction potentials and ACh-induced electrical changes. The results of substitution studies using isethionate for chloride provided evidence that a chloride conductance contributes to the resting potential. The extrapolated reversal potential for ACh-induced depolarization was positive to the resting potential. Isethionate substitution inhibited ACh-induced depolarization, consistent with a contribution from increased Cl- conductance to the depolarization induced by ACh. However, closure of K+ channels and opening of a nonspecific cation channel could also contribute to depolarization. Further study of the effects of isethionate substitution during prolonged tissue exposure to chloride-free medium showed that retention or the accumulation of Ca2+ in intracellular stores was impaired. We conclude that effects of chloride deprivation on responses to ACh may reflect an early increase in Cl- conductance, but longer term changes reflect the requirement for this anion to maintain internal Ca2+ stores.en_US
dc.languageengen_US
dc.publisherN R C Research Press. The Journal's web site is located at http://pubs.nrc-cnrc.gc.ca/cgi-bin/rp/rp2_desc_e?cjppen_US
dc.relation.ispartofCanadian Journal of Physiology and Pharmacologyen_US
dc.subject.meshAcetylcholine - Pharmacologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntiporters - Drug Effectsen_US
dc.subject.meshCalcium - Metabolism - Physiologyen_US
dc.subject.meshChloride Channels - Drug Effects - Physiologyen_US
dc.subject.meshChloride-Bicarbonate Antiportersen_US
dc.subject.meshChlorides - Physiologyen_US
dc.subject.meshDogsen_US
dc.subject.meshFemaleen_US
dc.subject.meshIntracellular Fluid - Metabolismen_US
dc.subject.meshIsethionic Acid - Pharmacologyen_US
dc.subject.meshMaleen_US
dc.subject.meshMembrane Potentials - Drug Effects - Physiologyen_US
dc.subject.meshMuscle Contraction - Drug Effects - Physiologyen_US
dc.subject.meshMuscle, Smooth - Drug Effects - Physiologyen_US
dc.subject.meshNeural Conduction - Drug Effects - Physiologyen_US
dc.subject.meshSodium - Physiologyen_US
dc.subject.meshTime Factorsen_US
dc.subject.meshTrachea - Drug Effects - Physiologyen_US
dc.titleChloride and depolarization by acetylcholine in canine airway smooth muscleen_US
dc.typeArticleen_US
dc.identifier.emailBourreau, JP:bourreau@hkucc.hku.hken_US
dc.identifier.authorityBourreau, JP=rp00389en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1139/y93-044-
dc.identifier.pmid8402393-
dc.identifier.scopuseid_2-s2.0-0027205606en_US
dc.identifier.volume71en_US
dc.identifier.issue3-4en_US
dc.identifier.spage284en_US
dc.identifier.epage292en_US
dc.identifier.isiWOS:A1993LN77500016-
dc.publisher.placeCanadaen_US
dc.identifier.scopusauthoridDaniel, EE=35474017600en_US
dc.identifier.scopusauthoridJury, J=7004287397en_US
dc.identifier.scopusauthoridBourreau, JP=7003927886en_US
dc.identifier.scopusauthoridJager, L=7103299901en_US

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