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Article: Louping ill virus envelope protein expressed by recombinant baculovirus and vaccinia virus fails to stimulate protective immunity

TitleLouping ill virus envelope protein expressed by recombinant baculovirus and vaccinia virus fails to stimulate protective immunity
Authors
Issue Date1992
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/virusres
Citation
Virus Research, 1992, v. 26 n. 3, p. 213-229 How to Cite?
AbstractWe have constructed recombinant baculoviruses and vaccinia viruses containing cloned DNA, encoding either the envelope protein alone or all of the structural proteins (core, membrane and envelope) of louping ill virus. Glycosylated viral envelope protein, presented both inside and on the surface of insect and mammalian cells, was expressed by all four recombinant viruses. Differences in antigenic presentation of the envelope protein were observed between the envelope protein and structural protein constructs as well as between the insect and mammalian cell expression systems. Despite the expression of epitopes known to elicit neutralizing and protective antibodies when present in authentic antigen, the recombinant envelope protein expressed by either vector failed to induce, in mice or rabbits, either neutralizing or protective antibodies against louping ill virus.
Persistent Identifierhttp://hdl.handle.net/10722/171569
ISSN
2015 Impact Factor: 2.526
2015 SCImago Journal Rankings: 1.259
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorShiu, SYWen_US
dc.contributor.authorReid, HWen_US
dc.contributor.authorGould, EAen_US
dc.date.accessioned2012-10-30T06:15:43Z-
dc.date.available2012-10-30T06:15:43Z-
dc.date.issued1992en_US
dc.identifier.citationVirus Research, 1992, v. 26 n. 3, p. 213-229en_US
dc.identifier.issn0168-1702en_US
dc.identifier.urihttp://hdl.handle.net/10722/171569-
dc.description.abstractWe have constructed recombinant baculoviruses and vaccinia viruses containing cloned DNA, encoding either the envelope protein alone or all of the structural proteins (core, membrane and envelope) of louping ill virus. Glycosylated viral envelope protein, presented both inside and on the surface of insect and mammalian cells, was expressed by all four recombinant viruses. Differences in antigenic presentation of the envelope protein were observed between the envelope protein and structural protein constructs as well as between the insect and mammalian cell expression systems. Despite the expression of epitopes known to elicit neutralizing and protective antibodies when present in authentic antigen, the recombinant envelope protein expressed by either vector failed to induce, in mice or rabbits, either neutralizing or protective antibodies against louping ill virus.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/virusresen_US
dc.relation.ispartofVirus Researchen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodies, Viral - Biosynthesisen_US
dc.subject.meshBaculoviridae - Genetics - Immunologyen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshEncephalitis Viruses, Tick-Borne - Genetics - Growth & Development - Immunologyen_US
dc.subject.meshGenes, Viralen_US
dc.subject.meshGenetic Vectorsen_US
dc.subject.meshImmune Sera - Chemistryen_US
dc.subject.meshImmunization, Passiveen_US
dc.subject.meshLouping Ill - Immunologyen_US
dc.subject.meshMiceen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshMoths - Geneticsen_US
dc.subject.meshNeutralization Testsen_US
dc.subject.meshRabbitsen_US
dc.subject.meshRecombinant Proteins - Immunologyen_US
dc.subject.meshTogaviridae Infections - Immunologyen_US
dc.subject.meshTunicamycin - Pharmacologyen_US
dc.subject.meshVaccinia Virus - Genetics - Immunologyen_US
dc.subject.meshViral Envelope Proteins - Biosynthesis - Immunologyen_US
dc.titleLouping ill virus envelope protein expressed by recombinant baculovirus and vaccinia virus fails to stimulate protective immunityen_US
dc.typeArticleen_US
dc.identifier.emailShiu, SYW:sywshiu@hkucc.hku.hken_US
dc.identifier.authorityShiu, SYW=rp00384en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0168-1702(92)90014-Zen_US
dc.identifier.pmid1337231-
dc.identifier.scopuseid_2-s2.0-0026621017en_US
dc.identifier.volume26en_US
dc.identifier.issue3en_US
dc.identifier.spage213en_US
dc.identifier.epage229en_US
dc.identifier.isiWOS:A1992KF97600003-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridShiu, SYW=7005550655en_US
dc.identifier.scopusauthoridReid, HW=7101876867en_US
dc.identifier.scopusauthoridGould, EA=7101857623en_US

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