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Article: Acetylcholine Ca2+ stores refilling directly involves a dihydropyridine-sensitive channel in dog trachea

TitleAcetylcholine Ca2+ stores refilling directly involves a dihydropyridine-sensitive channel in dog trachea
Authors
Issue Date1991
PublisherAmerican Physiological Society. The Journal's web site is located at http://ajpcon.physiology.org/
Citation
American Journal Of Physiology - Cell Physiology, 1991, v. 261 n. 3 30-3, p. C497-C505 How to Cite?
AbstractRepetitive stimulation of the smooth muscle with acetylcholine (ACh) in the continuous presence of nifedipine resulted in a progressive decrease in the developed tension. This was associated with a decrease in the content of the agonist-sensitive intracellular Ca2+ stores. Agonist-sensitive internal Ca2+ stores appeared to be readily depleted by successive or prolonged agonist stimulation in Ca2+-free medium. The refilling of the empty stores when the muscle is at rest required extracellular Ca2+, was decreased by nifedipine, and was increased by BAY K 8644 and by increased external Ca2+ concentration. Refilling of stores during ACh stimulation in Ca2+-containing medium was decreased by nifedipine and by cyclopiazonic acid (CPA), an inhibitor of the sarcoplasmic reticulum (SR) Ca2+ pump, and was potentiated by BAY K 8644. BAY K 8644 reversed the inhibitory effect of CPA on stores Ca2+ refilling. Ryanodine in normal Krebs increased muscle resting tension, an effect not observed in Ca2+-free medium, blocked by nifedipine and enhanced by BAY K 8644. We propose that the refilling of ACh-sensitive internal Ca2+ stores involves two distinct pathways, one dependent on the uptake of cytosolic Ca2+ via a CPA-sensitive SR Ca2+-adenosinetriphosphatase, and the other pathway dependent on extracellular Ca2+ influx via a dihydropyridine-sensitive Ca2+ channel and is CPA insensitive. The refilling pathway between plasmalemma and SR may involve a plasmalemma L-type Ca2+ channel (dihydropyridine sensitive) and the SR Ca2+ release channel (ryanodine sensitive).
Persistent Identifierhttp://hdl.handle.net/10722/171557
ISSN
1998 Impact Factor: 3.077
2004 SCImago Journal Rankings: 1.102
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorBourreau, JPen_US
dc.contributor.authorAbela, APen_US
dc.contributor.authorKwan, CYen_US
dc.contributor.authorDaniel, EEen_US
dc.date.accessioned2012-10-30T06:15:40Z-
dc.date.available2012-10-30T06:15:40Z-
dc.date.issued1991en_US
dc.identifier.citationAmerican Journal Of Physiology - Cell Physiology, 1991, v. 261 n. 3 30-3, p. C497-C505en_US
dc.identifier.issn0002-9513en_US
dc.identifier.urihttp://hdl.handle.net/10722/171557-
dc.description.abstractRepetitive stimulation of the smooth muscle with acetylcholine (ACh) in the continuous presence of nifedipine resulted in a progressive decrease in the developed tension. This was associated with a decrease in the content of the agonist-sensitive intracellular Ca2+ stores. Agonist-sensitive internal Ca2+ stores appeared to be readily depleted by successive or prolonged agonist stimulation in Ca2+-free medium. The refilling of the empty stores when the muscle is at rest required extracellular Ca2+, was decreased by nifedipine, and was increased by BAY K 8644 and by increased external Ca2+ concentration. Refilling of stores during ACh stimulation in Ca2+-containing medium was decreased by nifedipine and by cyclopiazonic acid (CPA), an inhibitor of the sarcoplasmic reticulum (SR) Ca2+ pump, and was potentiated by BAY K 8644. BAY K 8644 reversed the inhibitory effect of CPA on stores Ca2+ refilling. Ryanodine in normal Krebs increased muscle resting tension, an effect not observed in Ca2+-free medium, blocked by nifedipine and enhanced by BAY K 8644. We propose that the refilling of ACh-sensitive internal Ca2+ stores involves two distinct pathways, one dependent on the uptake of cytosolic Ca2+ via a CPA-sensitive SR Ca2+-adenosinetriphosphatase, and the other pathway dependent on extracellular Ca2+ influx via a dihydropyridine-sensitive Ca2+ channel and is CPA insensitive. The refilling pathway between plasmalemma and SR may involve a plasmalemma L-type Ca2+ channel (dihydropyridine sensitive) and the SR Ca2+ release channel (ryanodine sensitive).en_US
dc.languageengen_US
dc.publisherAmerican Physiological Society. The Journal's web site is located at http://ajpcon.physiology.org/en_US
dc.relation.ispartofAmerican Journal of Physiology - Cell Physiologyen_US
dc.subject.mesh3-Pyridinecarboxylic Acid, 1,4-Dihydro-2,6-Dimethyl-5-Nitro-4-(2-(Trifluoromethyl)Phenyl)-, Methyl Ester - Pharmacologyen_US
dc.subject.meshAcetylcholine - Pharmacologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCalcium - Metabolism - Pharmacologyen_US
dc.subject.meshCalcium Channels - Drug Effects - Physiologyen_US
dc.subject.meshDogsen_US
dc.subject.meshEgtazic Acid - Pharmacologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshKineticsen_US
dc.subject.meshMaleen_US
dc.subject.meshMembrane Potentials - Drug Effectsen_US
dc.subject.meshMuscle Contraction - Drug Effectsen_US
dc.subject.meshMuscle, Smooth - Drug Effects - Physiologyen_US
dc.subject.meshNifedipine - Pharmacologyen_US
dc.subject.meshRyanodine - Pharmacologyen_US
dc.subject.meshTrachea - Drug Effects - Physiologyen_US
dc.titleAcetylcholine Ca2+ stores refilling directly involves a dihydropyridine-sensitive channel in dog tracheaen_US
dc.typeArticleen_US
dc.identifier.emailBourreau, JP:bourreau@hkucc.hku.hken_US
dc.identifier.authorityBourreau, JP=rp00389en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid1716053-
dc.identifier.scopuseid_2-s2.0-0025990128en_US
dc.identifier.volume261en_US
dc.identifier.issue3 30-3en_US
dc.identifier.spageC497en_US
dc.identifier.epageC505en_US
dc.identifier.isiWOS:A1991GE63700015-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridBourreau, JP=7003927886en_US
dc.identifier.scopusauthoridAbela, AP=6602668587en_US
dc.identifier.scopusauthoridKwan, CY=7201421224en_US
dc.identifier.scopusauthoridDaniel, EE=35474017600en_US

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