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Article: Internal GTP stimulates the speract receptor mediated voltage changes in sea urchin spermatozoa membrane vesicles

TitleInternal GTP stimulates the speract receptor mediated voltage changes in sea urchin spermatozoa membrane vesicles
Authors
Issue Date1988
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ydbio
Citation
Developmental Biology, 1988, v. 126 n. 1, p. 91-97 How to Cite?
AbstractA voltage-sensitive Na+/H+ exchanger in the flagellar membrane is responsible for regulating the intracellular pH of the sea urchin spermatozoa. A previous study has shown that the egg peptide speract can modulate this Na+/H+ exchanger through its hyperpolarizing effect on the membrane potential. The effect of GTP on this speract receptor mediated process is investigated in this study. Plasma membrane vesicles with an outwardly directed K+ gradient were prepared from the isolated flagella by osmotic lysis. Vesicular membrane potential was monitored by a cationic probe, diS-C3-(5), and an anionic probe, diS-BA-C2-(3). Results show that the presence of internal GTP greatly stimulated the speract induced membrane hyperpolarization in this vesicle system. The analog GTPγS was not only active but could, by itself, induce partial hyperpolarization which was further enhanced by speract addition. Internal GDP was partially active in supporting the speract effect, whereas GDPβS, cGMP, GMP, and ATP were all inactive. The ionic selectivity of the speract effect was investigated by increasing the external concentration of various cations. K+ and Rb+ abolished the hyperpolarization while Cs+ had no effect. These results indicate that internal GTP is involved in the coupling between the speract receptor and the membrane hyperpolarization, which is most likely due to the activation of K+ selective channels.
Persistent Identifierhttp://hdl.handle.net/10722/171521
ISSN
2015 Impact Factor: 3.155
2015 SCImago Journal Rankings: 2.554
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLee, HCen_US
dc.date.accessioned2012-10-30T06:15:31Z-
dc.date.available2012-10-30T06:15:31Z-
dc.date.issued1988en_US
dc.identifier.citationDevelopmental Biology, 1988, v. 126 n. 1, p. 91-97en_US
dc.identifier.issn0012-1606en_US
dc.identifier.urihttp://hdl.handle.net/10722/171521-
dc.description.abstractA voltage-sensitive Na+/H+ exchanger in the flagellar membrane is responsible for regulating the intracellular pH of the sea urchin spermatozoa. A previous study has shown that the egg peptide speract can modulate this Na+/H+ exchanger through its hyperpolarizing effect on the membrane potential. The effect of GTP on this speract receptor mediated process is investigated in this study. Plasma membrane vesicles with an outwardly directed K+ gradient were prepared from the isolated flagella by osmotic lysis. Vesicular membrane potential was monitored by a cationic probe, diS-C3-(5), and an anionic probe, diS-BA-C2-(3). Results show that the presence of internal GTP greatly stimulated the speract induced membrane hyperpolarization in this vesicle system. The analog GTPγS was not only active but could, by itself, induce partial hyperpolarization which was further enhanced by speract addition. Internal GDP was partially active in supporting the speract effect, whereas GDPβS, cGMP, GMP, and ATP were all inactive. The ionic selectivity of the speract effect was investigated by increasing the external concentration of various cations. K+ and Rb+ abolished the hyperpolarization while Cs+ had no effect. These results indicate that internal GTP is involved in the coupling between the speract receptor and the membrane hyperpolarization, which is most likely due to the activation of K+ selective channels.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ydbioen_US
dc.relation.ispartofDevelopmental Biologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCell Membrane - Physiologyen_US
dc.subject.meshFlagella - Physiologyen_US
dc.subject.meshGuanosine Triphosphate - Metabolismen_US
dc.subject.meshIon Channels - Metabolismen_US
dc.subject.meshMaleen_US
dc.subject.meshMembrane Potentials - Drug Effectsen_US
dc.subject.meshOligopeptides - Pharmacologyen_US
dc.subject.meshPotassium - Metabolismen_US
dc.subject.meshReceptors, Cell Surface - Physiologyen_US
dc.subject.meshRubidium - Metabolismen_US
dc.subject.meshSea Urchinsen_US
dc.subject.meshSpermatozoa - Physiology - Ultrastructureen_US
dc.subject.meshValinomycin - Pharmacologyen_US
dc.titleInternal GTP stimulates the speract receptor mediated voltage changes in sea urchin spermatozoa membrane vesiclesen_US
dc.typeArticleen_US
dc.identifier.emailLee, HC:leehc@hku.hken_US
dc.identifier.authorityLee, HC=rp00545en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0012-1606(88)90242-4-
dc.identifier.pmid2449375-
dc.identifier.scopuseid_2-s2.0-0023866262en_US
dc.identifier.volume126en_US
dc.identifier.issue1en_US
dc.identifier.spage91en_US
dc.identifier.epage97en_US
dc.identifier.isiWOS:A1988M442600011-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLee, HC=26642959100en_US

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