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Article: A volatile inhibitor immobilizes sea urchin sperm in semen by depressing the intracellular pH

TitleA volatile inhibitor immobilizes sea urchin sperm in semen by depressing the intracellular pH
Authors
Issue Date1983
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ydbio
Citation
Developmental Biology, 1983, v. 98 n. 2, p. 493-501 How to Cite?
AbstractSea urchin spermatozoa are normally immotile in semen, but motility can be initiated by increasing gas flow over the semen-for example, by blowing N2 gas over a thin layer of semen. This result indicates that sperm motility is not O2 limited and suggests that seminal fluid contains a volatile inhibitor of motility which is responsible for the paralysis of sperm in semen. This inhibitor might be carbon dioxide, which reversibly immobilizes sperm. 31P-NMR measurements of pH show that the sperm intracellular pH (pHi) increases by 0.36 pH unit upon dilution of semen into seawater. Since previous studies have shown that this magnitude of pH increase is sufficient to trigger sperm motility, we suggest that the volatile inhibitor is inhibiting sperm motility in semen by depressing the pHi. A simple hypothesis that explains these observations is that the volatile motility inhibitor is CO2, which could acidify pHi as a diffusable weak acid. In this regard, sperm diluted into seawater release acid, and this acid release is related to the pHi increase and motility initiation. In fact, nearly half of the acid released by sperm upon dilution is volatile and may therefore be due to CO2 efflux. Most of the acid, however, cannot be attributed to CO2 release because it is not volatile. Thus, when sperm are diluted into seawater, they raise their pHi by releasing CO2 and protons from the cytoplasm into the surrounding seawater. © 1983.
Persistent Identifierhttp://hdl.handle.net/10722/171482
ISSN
2015 Impact Factor: 3.155
2015 SCImago Journal Rankings: 2.554
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorJohnson, CHen_US
dc.contributor.authorClapper, DLen_US
dc.contributor.authorWinkler, MMen_US
dc.contributor.authorLee, HCen_US
dc.contributor.authorEpel, Den_US
dc.date.accessioned2012-10-30T06:15:23Z-
dc.date.available2012-10-30T06:15:23Z-
dc.date.issued1983en_US
dc.identifier.citationDevelopmental Biology, 1983, v. 98 n. 2, p. 493-501en_US
dc.identifier.issn0012-1606en_US
dc.identifier.urihttp://hdl.handle.net/10722/171482-
dc.description.abstractSea urchin spermatozoa are normally immotile in semen, but motility can be initiated by increasing gas flow over the semen-for example, by blowing N2 gas over a thin layer of semen. This result indicates that sperm motility is not O2 limited and suggests that seminal fluid contains a volatile inhibitor of motility which is responsible for the paralysis of sperm in semen. This inhibitor might be carbon dioxide, which reversibly immobilizes sperm. 31P-NMR measurements of pH show that the sperm intracellular pH (pHi) increases by 0.36 pH unit upon dilution of semen into seawater. Since previous studies have shown that this magnitude of pH increase is sufficient to trigger sperm motility, we suggest that the volatile inhibitor is inhibiting sperm motility in semen by depressing the pHi. A simple hypothesis that explains these observations is that the volatile motility inhibitor is CO2, which could acidify pHi as a diffusable weak acid. In this regard, sperm diluted into seawater release acid, and this acid release is related to the pHi increase and motility initiation. In fact, nearly half of the acid released by sperm upon dilution is volatile and may therefore be due to CO2 efflux. Most of the acid, however, cannot be attributed to CO2 release because it is not volatile. Thus, when sperm are diluted into seawater, they raise their pHi by releasing CO2 and protons from the cytoplasm into the surrounding seawater. © 1983.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ydbioen_US
dc.relation.ispartofDevelopmental Biologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCarbon Dioxide - Physiologyen_US
dc.subject.meshHydrogen-Ion Concentrationen_US
dc.subject.meshMagnetic Resonance Spectroscopyen_US
dc.subject.meshMaleen_US
dc.subject.meshProtonsen_US
dc.subject.meshSemen - Analysis - Physiologyen_US
dc.subject.meshSperm Motilityen_US
dc.subject.meshSpermatozoa - Physiologyen_US
dc.titleA volatile inhibitor immobilizes sea urchin sperm in semen by depressing the intracellular pHen_US
dc.typeArticleen_US
dc.identifier.emailLee, HC:leehc@hku.hken_US
dc.identifier.authorityLee, HC=rp00545en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0012-1606(83)90378-0-
dc.identifier.pmid6307774-
dc.identifier.scopuseid_2-s2.0-0020803428en_US
dc.identifier.volume98en_US
dc.identifier.issue2en_US
dc.identifier.spage493en_US
dc.identifier.epage501en_US
dc.identifier.isiWOS:A1983RC35100023-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridJohnson, CH=7405669143en_US
dc.identifier.scopusauthoridClapper, DL=6701733043en_US
dc.identifier.scopusauthoridWinkler, MM=7201542951en_US
dc.identifier.scopusauthoridLee, HC=26642959100en_US
dc.identifier.scopusauthoridEpel, D=7005204813en_US

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