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Article: Changes of internal pH associated with initiation of motility and acrosome reaction of sea urchin sperm

TitleChanges of internal pH associated with initiation of motility and acrosome reaction of sea urchin sperm
Authors
Issue Date1983
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ydbio
Citation
Developmental Biology, 1983, v. 95 n. 1, p. 31-45 How to Cite?
AbstractThe changes in the intracellular pH (pH(i)) of sea urchin sperm associated with motility initiation and acrosome reaction were investigated using uptake of two different probes; 9-aminoacridine and methylamine, as a qualitative index. Sperm suspended in Na+-free sea water were immotile and able to concentrate these amines 20-fold or greater indicating that pH(i) is more acidic than the external medium (PH(o)=7.7). This uptake ratio was essentially constant over a wide range of probe and sperm concentrations. Discharge of the pH gradient with specific ionophores (nigericin, monensin, and tetrachlorosalicylanilide) or nonspecifically using low concentration of detergents (Triton X-100 and lysolecithin) all resulted in the release of the probes indicating they are indeed sensing the pH gradient across the sperm membrane. Addition of Na+ to sperm suspended in Na+- free sea water resulted in activation of motility with concomitant efflux of the probes indicating the alkalinization of pH(i) by 0.4-0.5 pH units. That this pH(i) change is the causal trigger of motility was suggested by experiments using NH4Cl and nigericin, which increased the pH(i) and resulted in activation of motility in the absence of Na+. When sperm were directly diluted into artificial sea water (motility activated), a slow reacidification of pH(i) was observed in one species of sea urchin (L. pictus) but not in the other (S. purpuratus). This acidification could be blocked by mitochondrial inhibitors, verapamil, or the removal of external calcium suggesting that the increase in metabolic activity stimulated by the influx of Ca2+ is responsible for the reacidification. Induction of acrosome reaction further alkalinized the pH(i) by about 0.16 pH units and was also followed by prolonged reacidification which correlated with the observed increase in Ca2+ uptake. Either mitochondrial agents or the removal of external Ca2+ could also block this pH(i) change suggesting a similar mechanism is involved.
Persistent Identifierhttp://hdl.handle.net/10722/171481
ISSN
2015 Impact Factor: 3.155
2015 SCImago Journal Rankings: 2.554
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLee, HCen_US
dc.contributor.authorJohnson, Cen_US
dc.contributor.authorEpel, Den_US
dc.date.accessioned2012-10-30T06:15:22Z-
dc.date.available2012-10-30T06:15:22Z-
dc.date.issued1983en_US
dc.identifier.citationDevelopmental Biology, 1983, v. 95 n. 1, p. 31-45en_US
dc.identifier.issn0012-1606en_US
dc.identifier.urihttp://hdl.handle.net/10722/171481-
dc.description.abstractThe changes in the intracellular pH (pH(i)) of sea urchin sperm associated with motility initiation and acrosome reaction were investigated using uptake of two different probes; 9-aminoacridine and methylamine, as a qualitative index. Sperm suspended in Na+-free sea water were immotile and able to concentrate these amines 20-fold or greater indicating that pH(i) is more acidic than the external medium (PH(o)=7.7). This uptake ratio was essentially constant over a wide range of probe and sperm concentrations. Discharge of the pH gradient with specific ionophores (nigericin, monensin, and tetrachlorosalicylanilide) or nonspecifically using low concentration of detergents (Triton X-100 and lysolecithin) all resulted in the release of the probes indicating they are indeed sensing the pH gradient across the sperm membrane. Addition of Na+ to sperm suspended in Na+- free sea water resulted in activation of motility with concomitant efflux of the probes indicating the alkalinization of pH(i) by 0.4-0.5 pH units. That this pH(i) change is the causal trigger of motility was suggested by experiments using NH4Cl and nigericin, which increased the pH(i) and resulted in activation of motility in the absence of Na+. When sperm were directly diluted into artificial sea water (motility activated), a slow reacidification of pH(i) was observed in one species of sea urchin (L. pictus) but not in the other (S. purpuratus). This acidification could be blocked by mitochondrial inhibitors, verapamil, or the removal of external calcium suggesting that the increase in metabolic activity stimulated by the influx of Ca2+ is responsible for the reacidification. Induction of acrosome reaction further alkalinized the pH(i) by about 0.16 pH units and was also followed by prolonged reacidification which correlated with the observed increase in Ca2+ uptake. Either mitochondrial agents or the removal of external Ca2+ could also block this pH(i) change suggesting a similar mechanism is involved.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ydbioen_US
dc.relation.ispartofDevelopmental Biologyen_US
dc.subject.meshAcrosome - Physiologyen_US
dc.subject.meshAminacrineen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCell Membrane - Metabolismen_US
dc.subject.meshHydrogen-Ion Concentrationen_US
dc.subject.meshIntracellular Fluid - Metabolismen_US
dc.subject.meshMaleen_US
dc.subject.meshMethylaminesen_US
dc.subject.meshSea Urchins - Physiologyen_US
dc.subject.meshSodium - Metabolismen_US
dc.subject.meshSperm Motilityen_US
dc.subject.meshSpermatozoa - Metabolism - Physiologyen_US
dc.titleChanges of internal pH associated with initiation of motility and acrosome reaction of sea urchin spermen_US
dc.typeArticleen_US
dc.identifier.emailLee, HC:leehc@hku.hken_US
dc.identifier.authorityLee, HC=rp00545en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0012-1606(83)90004-0-
dc.identifier.pmid6825930-
dc.identifier.scopuseid_2-s2.0-0020671590en_US
dc.identifier.volume95en_US
dc.identifier.issue1en_US
dc.identifier.spage31en_US
dc.identifier.epage45en_US
dc.identifier.isiWOS:A1983PZ92000003-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLee, HC=26642959100en_US
dc.identifier.scopusauthoridJohnson, C=7405669143en_US
dc.identifier.scopusauthoridEpel, D=7005204813en_US

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