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Article: Changes in intracellular acidic compartments in sea urchin eggs after activation

TitleChanges in intracellular acidic compartments in sea urchin eggs after activation
Authors
Issue Date1983
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ydbio
Citation
Developmental Biology, 1983, v. 98 n. 2, p. 446-454 How to Cite?
AbstractAcridine orange (AO) was used as a vital probe for looking at acidic intracellular compartments in sea urchin eggs. This weak base is concentrated by acidic compartments, shifting its fluorescence from green to red due to the formation of dye aggregates. Fertilization or parthenogenetic activation with ionophore A23187 resulted in the appearance of orange fluorescent granules of sizes ranging from 1 to 2 μm at the cortical region of the egg. In one species of sea urchin (Lytechinus pictus), these granules migrate inward before cell division and associate with the forming mitotic apparatus. Treatments that discharge the transmembrane pH gradient (NH4Cl, nigericin, monensin, and acidic external pH) eliminate the orange fluorescence, indicating they are acidic compartments. Spectrofluorimetric measurements showed a decrease in monomer fluorescence accompanying egg activation which is reversible by similar treatments as seen with the fluorescence microscopic observations. Stratified eggs which were subsequently fertilized had acidic granules concentrated at the centripetal pole. This allowed the electron microscopic identification of the granules and showed they are present in the unfertilized egg, although not able to concentrate the AO. Activation of eggs in the absence of Na+ prevented the cytoplasmic alkalinization and also inhibited the appearance of acidic granules. The results indicate that the internal pH rises after egg activation triggers the acidification of these granules. Their possible functions, as in intracellular pH regulation, are discussed.
Persistent Identifierhttp://hdl.handle.net/10722/171480
ISSN
2015 Impact Factor: 3.155
2015 SCImago Journal Rankings: 2.554
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLee, HCen_US
dc.contributor.authorEpel, Den_US
dc.date.accessioned2012-10-30T06:15:22Z-
dc.date.available2012-10-30T06:15:22Z-
dc.date.issued1983en_US
dc.identifier.citationDevelopmental Biology, 1983, v. 98 n. 2, p. 446-454en_US
dc.identifier.issn0012-1606en_US
dc.identifier.urihttp://hdl.handle.net/10722/171480-
dc.description.abstractAcridine orange (AO) was used as a vital probe for looking at acidic intracellular compartments in sea urchin eggs. This weak base is concentrated by acidic compartments, shifting its fluorescence from green to red due to the formation of dye aggregates. Fertilization or parthenogenetic activation with ionophore A23187 resulted in the appearance of orange fluorescent granules of sizes ranging from 1 to 2 μm at the cortical region of the egg. In one species of sea urchin (Lytechinus pictus), these granules migrate inward before cell division and associate with the forming mitotic apparatus. Treatments that discharge the transmembrane pH gradient (NH4Cl, nigericin, monensin, and acidic external pH) eliminate the orange fluorescence, indicating they are acidic compartments. Spectrofluorimetric measurements showed a decrease in monomer fluorescence accompanying egg activation which is reversible by similar treatments as seen with the fluorescence microscopic observations. Stratified eggs which were subsequently fertilized had acidic granules concentrated at the centripetal pole. This allowed the electron microscopic identification of the granules and showed they are present in the unfertilized egg, although not able to concentrate the AO. Activation of eggs in the absence of Na+ prevented the cytoplasmic alkalinization and also inhibited the appearance of acidic granules. The results indicate that the internal pH rises after egg activation triggers the acidification of these granules. Their possible functions, as in intracellular pH regulation, are discussed.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ydbioen_US
dc.relation.ispartofDevelopmental Biologyen_US
dc.subject.meshAcridine Orange - Diagnostic Useen_US
dc.subject.meshAmmonium Chloride - Pharmacologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCalcimycin - Pharmacologyen_US
dc.subject.meshCell Compartmentationen_US
dc.subject.meshCytoplasmic Granules - Physiologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshFertilizationen_US
dc.subject.meshHydrogen-Ion Concentrationen_US
dc.subject.meshMaleen_US
dc.subject.meshOvum - Physiologyen_US
dc.subject.meshParthenogenesisen_US
dc.subject.meshSea Urchins - Physiologyen_US
dc.subject.meshSodium Chloride - Pharmacologyen_US
dc.subject.meshZygote - Physiologyen_US
dc.titleChanges in intracellular acidic compartments in sea urchin eggs after activationen_US
dc.typeArticleen_US
dc.identifier.emailLee, HC:leehc@hku.hken_US
dc.identifier.authorityLee, HC=rp00545en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0012-1606(83)90374-3-
dc.identifier.pmid6409692-
dc.identifier.scopuseid_2-s2.0-0020606772en_US
dc.identifier.volume98en_US
dc.identifier.issue2en_US
dc.identifier.spage446en_US
dc.identifier.epage454en_US
dc.identifier.isiWOS:A1983RC35100019-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLee, HC=26642959100en_US
dc.identifier.scopusauthoridEpel, D=7005204813en_US

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