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Article: Inhibition of catechol-O-methyltransferase by gossypol: The effect of plasma proteins

TitleInhibition of catechol-O-methyltransferase by gossypol: The effect of plasma proteins
Authors
Issue Date1982
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/contraception
Citation
Contraception, 1982, v. 26 n. 5, p. 515-519 How to Cite?
AbstractCatechol-O-methyltransferase (COMT) is a key enzyme in the metabolism of catecholamines. As gossypol, a recent male contraceptive, has been reported to inhibit some respiratory enzymes in vitro, we have studied its effect on rat liver COMT activity. Varying concentrations of gossypol acetic acid were incubated with 3H-methyl-S-adenosyl-methionine, COMT, and different concentrations of norepinephrine. It was found that addition of gossypol into the incubation medium greatly depressed COMT activity, with an I 50 of 0.01 mM. A series of inhibitory curves constructed from using different concentrations of the substrate suggested that the inhibition was non-competitive. Addition of blood serum or BSA significantly reduced the inhibitory effect of gossypol. We concluded that gossypol may markedly decrease COMT activity through non-competitive inhibition. Since various organs of the rat have been shown to accumulate gossypol, ingestion of gossypol for the purpose of fertility control may have important effects on the metabolism of catecholamine.
Persistent Identifierhttp://hdl.handle.net/10722/171476
ISSN
2015 Impact Factor: 2.788
2015 SCImago Journal Rankings: 1.557
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorTang, Fen_US
dc.contributor.authorTsang, AYFen_US
dc.contributor.authorLee, Cen_US
dc.contributor.authorWong, PYDen_US
dc.date.accessioned2012-10-30T06:15:21Z-
dc.date.available2012-10-30T06:15:21Z-
dc.date.issued1982en_US
dc.identifier.citationContraception, 1982, v. 26 n. 5, p. 515-519en_US
dc.identifier.issn0010-7824en_US
dc.identifier.urihttp://hdl.handle.net/10722/171476-
dc.description.abstractCatechol-O-methyltransferase (COMT) is a key enzyme in the metabolism of catecholamines. As gossypol, a recent male contraceptive, has been reported to inhibit some respiratory enzymes in vitro, we have studied its effect on rat liver COMT activity. Varying concentrations of gossypol acetic acid were incubated with 3H-methyl-S-adenosyl-methionine, COMT, and different concentrations of norepinephrine. It was found that addition of gossypol into the incubation medium greatly depressed COMT activity, with an I 50 of 0.01 mM. A series of inhibitory curves constructed from using different concentrations of the substrate suggested that the inhibition was non-competitive. Addition of blood serum or BSA significantly reduced the inhibitory effect of gossypol. We concluded that gossypol may markedly decrease COMT activity through non-competitive inhibition. Since various organs of the rat have been shown to accumulate gossypol, ingestion of gossypol for the purpose of fertility control may have important effects on the metabolism of catecholamine.en_US
dc.languageengen_US
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/contraceptionen_US
dc.relation.ispartofContraceptionen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBlood Proteins - Metabolismen_US
dc.subject.meshCatechol O-Methyltransferase - Antagonists & Inhibitorsen_US
dc.subject.meshCatecholamines - Metabolismen_US
dc.subject.meshGossypol - Analogs & Derivatives - Pharmacologyen_US
dc.subject.meshLiver - Metabolismen_US
dc.subject.meshRatsen_US
dc.subject.meshSpermatocidal Agents - Pharmacologyen_US
dc.titleInhibition of catechol-O-methyltransferase by gossypol: The effect of plasma proteinsen_US
dc.typeArticleen_US
dc.identifier.emailTang, F:ftang@hkucc.hku.hken_US
dc.identifier.authorityTang, F=rp00327en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0010-7824(82)90150-0-
dc.identifier.pmid7160182-
dc.identifier.scopuseid_2-s2.0-0020365086en_US
dc.identifier.volume26en_US
dc.identifier.issue5en_US
dc.identifier.spage515en_US
dc.identifier.epage519en_US
dc.identifier.isiWOS:A1982PW85200008-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridTang, F=7201979770en_US
dc.identifier.scopusauthoridTsang, AYF=7006979237en_US
dc.identifier.scopusauthoridLee, C=7410144896en_US
dc.identifier.scopusauthoridWong, PYD=7403980262en_US

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