File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Osteoblasts on rod shaped hydroxyapatite nanoparticles incorporated pcl film provide an optimal osteogenic niche for stem cell differentiation

TitleOsteoblasts on rod shaped hydroxyapatite nanoparticles incorporated pcl film provide an optimal osteogenic niche for stem cell differentiation
Authors
Issue Date2011
PublisherMary Ann Liebert, Inc. Publishers. The Journal's web site is located at http://www.liebertpub.com/publication.aspx?pub_id=263
Citation
Tissue Engineering - Part A, 2011, v. 17 n. 11-12, p. 1651-1661 How to Cite?
AbstractAfter the clinical insertion of a bone biomaterial, the surrounding osteoblasts would migrate and attach to the implant surface and foster a microenvironment that largely determines the differentiation fate of the comigrated mesenchymal stem cells. Whether the fostered microenvironment is suitable for osteogenic differentiation of mesenchymal stem cells is critical for the subsequent osseointegration. In this study, we determined (1) how the spherical or rod-shaped hydroxyapatite nanoparticles (nHA) incorporated poly(E-caprolactone) (PCL) films (PCL-spherical nHA, PCL-rod nHA) interact with primary human osteoblasts (HOBs); (2) how the microenvironment rendered by their interaction affects osteogenic differentiation of adipose tissue-derived mesenchymal stem cells (ASCs). HOBs were seeded on PCL, PCL-spherical nHA, and PCL-rod nHA films, respectively. When cultured alone, the HOBs on PCL-rod nHA films showed most efficient osteoblastic differentiation compared with those on PCL or PCL-spherical nHA films. When cocultured with ASCs in an indirect coculture system, only the HOBs on PCL-rod nHA films up-regulated the gene expression of Runx2, bone sialoprotein, and osteocalcin of ASCs. Additionally, the HOBs on PCL-rod nHA films showed significant up-regulation of bone morphogenic protein 2 gene and protein expression and induced highest phosphorylated Smad1/5 protein level in ASCs. Treatment of the coculture medium with bone morphogenic protein 2 inhibitor (Noggin) largely abolished the osteogenic differentiation of the ASCs induced by the HOBs on PCL-rod nHA films. In conclusion, HOBs can not only best display their osteoblastic phenotype by culturing on PCL-rod nHA films but also render an optimal osteogenic niche for the differentiation of stem cells. © 2011 Mary Ann Liebert, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/171422
ISSN
2015 SCImago Journal Rankings: 1.500
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLu, Zen_US
dc.contributor.authorRoohaniEsfahani, SIen_US
dc.contributor.authorKwok, PCLen_US
dc.contributor.authorZreiqat, Hen_US
dc.date.accessioned2012-10-30T06:14:04Z-
dc.date.available2012-10-30T06:14:04Z-
dc.date.issued2011en_US
dc.identifier.citationTissue Engineering - Part A, 2011, v. 17 n. 11-12, p. 1651-1661en_US
dc.identifier.issn1937-3341en_US
dc.identifier.urihttp://hdl.handle.net/10722/171422-
dc.description.abstractAfter the clinical insertion of a bone biomaterial, the surrounding osteoblasts would migrate and attach to the implant surface and foster a microenvironment that largely determines the differentiation fate of the comigrated mesenchymal stem cells. Whether the fostered microenvironment is suitable for osteogenic differentiation of mesenchymal stem cells is critical for the subsequent osseointegration. In this study, we determined (1) how the spherical or rod-shaped hydroxyapatite nanoparticles (nHA) incorporated poly(E-caprolactone) (PCL) films (PCL-spherical nHA, PCL-rod nHA) interact with primary human osteoblasts (HOBs); (2) how the microenvironment rendered by their interaction affects osteogenic differentiation of adipose tissue-derived mesenchymal stem cells (ASCs). HOBs were seeded on PCL, PCL-spherical nHA, and PCL-rod nHA films, respectively. When cultured alone, the HOBs on PCL-rod nHA films showed most efficient osteoblastic differentiation compared with those on PCL or PCL-spherical nHA films. When cocultured with ASCs in an indirect coculture system, only the HOBs on PCL-rod nHA films up-regulated the gene expression of Runx2, bone sialoprotein, and osteocalcin of ASCs. Additionally, the HOBs on PCL-rod nHA films showed significant up-regulation of bone morphogenic protein 2 gene and protein expression and induced highest phosphorylated Smad1/5 protein level in ASCs. Treatment of the coculture medium with bone morphogenic protein 2 inhibitor (Noggin) largely abolished the osteogenic differentiation of the ASCs induced by the HOBs on PCL-rod nHA films. In conclusion, HOBs can not only best display their osteoblastic phenotype by culturing on PCL-rod nHA films but also render an optimal osteogenic niche for the differentiation of stem cells. © 2011 Mary Ann Liebert, Inc.en_US
dc.languageengen_US
dc.publisherMary Ann Liebert, Inc. Publishers. The Journal's web site is located at http://www.liebertpub.com/publication.aspx?pub_id=263en_US
dc.relation.ispartofTissue Engineering - Part Aen_US
dc.titleOsteoblasts on rod shaped hydroxyapatite nanoparticles incorporated pcl film provide an optimal osteogenic niche for stem cell differentiationen_US
dc.typeArticleen_US
dc.identifier.emailKwok, PCL:pclkwok@hku.hken_US
dc.identifier.authorityKwok, PCL=rp01540en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1089/ten.tea.2010.0567en_US
dc.identifier.pmid21306280-
dc.identifier.scopuseid_2-s2.0-79957593919en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79957593919&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume17en_US
dc.identifier.issue11-12en_US
dc.identifier.spage1651en_US
dc.identifier.epage1661en_US
dc.identifier.isiWOS:000291342600019-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLu, Z=16550184500en_US
dc.identifier.scopusauthoridRoohaniEsfahani, SI=35488794700en_US
dc.identifier.scopusauthoridKwok, PCL=12646007800en_US
dc.identifier.scopusauthoridZreiqat, H=7004263521en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats