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Article: Uridine adenosine tetraphosphate affects contractility of mouse aorta and decreases blood pressure in conscious rats and mice

TitleUridine adenosine tetraphosphate affects contractility of mouse aorta and decreases blood pressure in conscious rats and mice
Authors
Issue Date2010
PublisherWiley-Blackwell Publishing Ltd. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=1748-1708
Citation
Acta Physiologica, 2010, v. 200 n. 2, p. 171-179 How to Cite?
AbstractAim: In the anaesthetized rat, uridine adenosine tetraphosphate (Up 4A) is a circulating, endothelium-derived vasoconstrictor presumably operating as such in un-anaesthetized animals. The present study investigated the in vivo effects of Up4A in conscious mice and rats, and its direct vascular effects in the mouse aorta in vitro. Methods: In vivo, Up 4A was given as step-up infusion at rates of 8-512 nmol min -1 kg-1 for 30 min periods in chronically catheterized rodents. In vitro, the effect of Up4A on rings of mouse aortae mounted in a myograph was tested. Results: High doses of Up4A (mice: 512 nmol min-1 kg-1; rats: 128 nmol min-1 kg-1) caused hypotension (99 ± 4 to 64 ± 7 mmHg and 114 ± 3 to 108 ± 3 mmHg, respectively, both P < 0.01). In rats, Up4A significantly decreased sodium excretion by >75% and potassium excretion by ∼60% without significant changes in urine flow. Exposure of phenylephrine-contracted rings to increasing concentrations of Up4A elicited contraction at 10-7 and 10-6 mol L-1 (18 ± 2% and 76 ± 16% respectively); unexpectedly, 10-5 mol L-1 caused a biphasic response with a contraction (19 ± 6%) followed by a relaxation (-46 ± 6%). No relaxation was observed when the concentration was increased further. Bolus exposure to 10 -5 mol L-1 of Up4A caused contraction (+80 ± 2%). Added successively to untreated vessels, increasing concentrations of Up4A (10-7-10-5 mol L-1) induced a biphasic response of contraction followed by relaxation. Conclusion: Up 4A has direct biphasic effects on vascular smooth muscle of the mouse aorta but vasoconstriction dominates at low concentrations. In conscious rodents, step-up infusions of Up4A elicit hypotension and electrolyte retention. © 2010 Scandinavian Physiological Society.
Persistent Identifierhttp://hdl.handle.net/10722/171406
ISSN
2015 Impact Factor: 4.066
2015 SCImago Journal Rankings: 1.690
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHansen, PBen_US
dc.contributor.authorHristovska, Aen_US
dc.contributor.authorWolff, Hen_US
dc.contributor.authorVanhoutte, Pen_US
dc.contributor.authorJensen, BLen_US
dc.contributor.authorBie, Pen_US
dc.date.accessioned2012-10-30T06:13:57Z-
dc.date.available2012-10-30T06:13:57Z-
dc.date.issued2010en_US
dc.identifier.citationActa Physiologica, 2010, v. 200 n. 2, p. 171-179en_US
dc.identifier.issn1748-1708en_US
dc.identifier.urihttp://hdl.handle.net/10722/171406-
dc.description.abstractAim: In the anaesthetized rat, uridine adenosine tetraphosphate (Up 4A) is a circulating, endothelium-derived vasoconstrictor presumably operating as such in un-anaesthetized animals. The present study investigated the in vivo effects of Up4A in conscious mice and rats, and its direct vascular effects in the mouse aorta in vitro. Methods: In vivo, Up 4A was given as step-up infusion at rates of 8-512 nmol min -1 kg-1 for 30 min periods in chronically catheterized rodents. In vitro, the effect of Up4A on rings of mouse aortae mounted in a myograph was tested. Results: High doses of Up4A (mice: 512 nmol min-1 kg-1; rats: 128 nmol min-1 kg-1) caused hypotension (99 ± 4 to 64 ± 7 mmHg and 114 ± 3 to 108 ± 3 mmHg, respectively, both P < 0.01). In rats, Up4A significantly decreased sodium excretion by >75% and potassium excretion by ∼60% without significant changes in urine flow. Exposure of phenylephrine-contracted rings to increasing concentrations of Up4A elicited contraction at 10-7 and 10-6 mol L-1 (18 ± 2% and 76 ± 16% respectively); unexpectedly, 10-5 mol L-1 caused a biphasic response with a contraction (19 ± 6%) followed by a relaxation (-46 ± 6%). No relaxation was observed when the concentration was increased further. Bolus exposure to 10 -5 mol L-1 of Up4A caused contraction (+80 ± 2%). Added successively to untreated vessels, increasing concentrations of Up4A (10-7-10-5 mol L-1) induced a biphasic response of contraction followed by relaxation. Conclusion: Up 4A has direct biphasic effects on vascular smooth muscle of the mouse aorta but vasoconstriction dominates at low concentrations. In conscious rodents, step-up infusions of Up4A elicit hypotension and electrolyte retention. © 2010 Scandinavian Physiological Society.en_US
dc.languageengen_US
dc.publisherWiley-Blackwell Publishing Ltd. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=1748-1708en_US
dc.relation.ispartofActa Physiologicaen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAorta - Drug Effects - Physiologyen_US
dc.subject.meshBlood Pressure - Drug Effects - Physiologyen_US
dc.subject.meshConsciousnessen_US
dc.subject.meshDinucleoside Phosphates - Metabolism - Pharmacologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshKidney - Physiologyen_US
dc.subject.meshKidney Function Testsen_US
dc.subject.meshMaleen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred C57blen_US
dc.subject.meshMuscle Contraction - Drug Effects - Physiologyen_US
dc.subject.meshMuscle, Smooth, Vascular - Drug Effects - Physiologyen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshVasoconstriction - Drug Effects - Physiologyen_US
dc.titleUridine adenosine tetraphosphate affects contractility of mouse aorta and decreases blood pressure in conscious rats and miceen_US
dc.typeArticleen_US
dc.identifier.emailVanhoutte, P:vanhoutt@hku.hken_US
dc.identifier.authorityVanhoutte, P=rp00238en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1111/j.1748-1716.2010.02135.xen_US
dc.identifier.pmid20384597-
dc.identifier.scopuseid_2-s2.0-77956308849en_US
dc.identifier.hkuros183720-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77956308849&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume200en_US
dc.identifier.issue2en_US
dc.identifier.spage171en_US
dc.identifier.epage179en_US
dc.identifier.isiWOS:000281557700006-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridHansen, PB=35472646600en_US
dc.identifier.scopusauthoridHristovska, A=19933493100en_US
dc.identifier.scopusauthoridWolff, H=23994503400en_US
dc.identifier.scopusauthoridVanhoutte, P=7202304247en_US
dc.identifier.scopusauthoridJensen, BL=35502338900en_US
dc.identifier.scopusauthoridBie, P=7006398859en_US
dc.identifier.citeulike7857419-

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